Dan Bose

Link -> www.biorxiv.org/content/10.6...

A universal protein ladder for standardisation of diverse FRET assays https://www.biorxiv.org/content/10.64898/2026.03.05.709886v1

And all available through addgene for anyone who wants to give it a try...

It's been a privilege to be involved with this. #FRET approaches have so many potential applications for linking in vitro studies with in cell behaviours, but lacked a consistent benchmarking molecule that works across systems: not any more! #IDRs #smFRET #singlemolecule

Great to catch up with @sproullab.bsky.social today for the @drn-sheffield.bsky.social seminar series. Some really exciting stories that I can't wait to see in the wild!

The Dynamic Lives of Intrinsically Disordered Proteins Shapeshifting proteins challenge a long-standing maxim in biology.

Very nice article by Danielle Gerhard in The Scientist on

“The Dynamic Lives of Intrinsically Disordered Proteins”

with quotes from Gabi Heller, @alexholehouse.bsky.social and myself about our shared love of and fascination with these proteins

www.the-scientist.com/the-dynamic-...

For example, exquisite in vitro biochemistry and structural work on purified proteins is essential for pulling apart and understating mechanisms, but all too often the role of complex molecular environments in shaping these behaviours gets forgotten.

There's always a tension between building simple models to understand complex systems, and remembering that those models developed in isolation in reality exist and function in a highly complex context.

Really enjoyed this piece by @philipcball.bsky.social "Biology faces the problem that it is fundamentally complex (as well as merely complicated, meaning that there is a lot going on). " Applies well beyond genetics too.

Nice story- congratulations!

A pictographic model of chromatin folding in the nucleus. DNA wrapped around nucleosomes aggregates into nanoscale domains, whilst newly nucleosome depleted DNA that forms after acute degradation of FACT projects from these domains and engages in long-range interactions that can span TAD boundaries

New preprint from @anadopico.bsky.social (who I have had the great pleasure of supervising with Tom Milne) and our fantastic collaborators in the @davieslab.bsky.social

www.biorxiv.org/content/10.6...

#genomics #GeneRegulation #chromatin

(Also, kudos for the bird name!) #birdsinscience

It super exciting how quickly tools to unlock #IDR function are developing- can't wait to give this a try!! Congrats again!

Papers are like buses... You wait for ages, then two come along at once.

Huge congrats to @bornanovak.bsky.social and @jefflotthammer.bsky.social for pushing and driving every aspect of this work, preprinted ~1 year ago to the day (Friday before BPS), now published!

www.nature.com/articles/s41...

PDRA position still open in my lab for anyone who wants to solve some outstanding questions in limb development. We have a variety of models and approaches (everything from stem cells to chicks and catsharks). Please share!

Really important work unpicking how #IDR context imparts protein function- fantastic to see this finally out! Congratulations!

Interested in antisense oligonucleotides?
Check our story about downstream-of-gene ASOs (DG-ASOs)!
Together with the Roche Innovation Center we
identified transcription termination windows of protein-coding genes as ASO target regions.

link to BioRxiv:
www.biorxiv.org/content/10.6...

Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo Kinesin-1 is an essential anterograde microtubule motor protein. The core kinesin motor is a homodimer of two heavy chains; N-terminal motor domains hydrolyse ATP and walk along microtubules, whilst a...

I'm a bit late with this, but awesome to see it out in the wild! A real tour de force from @dozenoaks.bsky.social @timcraggs.bsky.social An amazing example of why we need to think more about flexibility in our biological questions! www.cell.com/iscience/ful...

Great to see @tslab.bsky.social on here at last! Check them out for all things #condenstaes and #RNAs

I am excited to share a new review @cp-molcell.bsky.social written in collaboration with @tanjamittag.bsky.social , Mikayla Eppert, and Ambuja Navalkar where we review the current evidence for and against the role of density transitions in regulating transcription www.cell.com/molecular-ce...

Finally comms from UKRI - on a Sunday evening. No mention of the hundreds of ECRs who will see their careers ended by these decisions and notably no explanation for MRC retrospectively applying cuts and wasting all the work that went into writing, reviewing and scoring applications - £millions

At a time when the UK should be capitalising on its global reputation as a great place to do science, this is an enormous own goal. I am so sorry for all those who jobs are going to be impacted.

Spot on.

Even if the changes are for the good (I doubt, but await the data) - implementing them like this is curtains for the careers of a bunch of postdoctoral scientists, and probably a final dissuasion for a bunch more.

And they talk and TALK about how much they want to support junior careers..

What these decisions basically say, is that unless you work on one of the few areas that a select few people have decided might be important because they could make £££, or that you are fortunate enough to work in a place that can afford a press department, then what you do is worthless.

On a personal level, I think one of the hardest things is the implicit statement that the work that so many 1000's of us do, and the significant expertise that we've spent entire careers developing and making sacrifices for, is just not valued at all, on any level, by #UKRI and by the government.

So much depressing news for UK science #funding at the moment; jobs lost, opportunities for future postdoc career development going up in smoke.

Advancing regulatory variant effect prediction with AlphaGenome - Nature AlphaGenome, a deep learning model that inputs 1-Mb DNA sequence to predict functional genomic tracks at single-base resolution across diverse modalities, outperforms existing models in variant effect...

I had intended to post something about this new Google DeepMind paper that appeared yesterday in Nature, but the press coverage has added to what there is to say. So this is a long 🧵
www.nature.com/articles/s41...

Significant cuts could damage the foundations of UK research - CaSE CaSE’s response to a letter sent by Science and Technology Facilities Council (STFC) Executive Chair Professor Michele Dougherty detailing major cuts to research funding.

CaSE: “UKRI need to explain how the cuts detailed in this letter are consistent with the commitment to protect curiosity-led research […]

“The information we have had so far is a failure in communication and transparency”

www.sciencecampaign.org.uk/press/detail...

Quite timely that Sir Ian Chapman, UKRI CEO, will be in front of the House of Commons Science, Innovation and Technology Committee on Tuesday next week… 👀

‘Grave’ concerns over applicant-led funds at UK research councils - Research Professional News Figures suggest reductions at MRC and BBSRC, with questions hanging over pending MRC grant applications

Full story by @isaacbarbosa1.bsky.social and me in today's Research Fortnight

www.researchprofessionalnews.com/rr-news-uk-r...