@daddea
cryoEM enthusiast, cryoET enthusiast soon. Last months as Research Assistant in WilsonLab, University of Hamburg, SPA cryoEM & ribosomes. Love Heterogeneity Analysis and continuous movements. Water diviner, always!
If you can, I would suggest you to search for “pasta dei cornuti” (literally, “pasta of the cheated husbands”). You would get some important and interesting insights about the recipe’s tradition -in Italy, at least
From what I remember (very little) was from wheat germ lysate, but yes, it was not in cells
Wow! From C39 to C13! Congratulations @olibclarke.bsky.social @fravallese.bsky.social
The vault particle is enclosed by a C13-symmetric cap with a positively charged exterior pubmed.ncbi.nlm.nih.gov/41706843/ #cryoEM
Oh, I remember that one, it was nice! Or a paper from Spirin, a NAR from 2016 I guess?
Pulsed #lasers integrated with #cryoEM: @fitzpatricklab.bsky.social postdoc Daniel Du aims to boost contrast in #cryoET zuckermaninstitute.columbia.edu/making-world-s-best-microscopes-biology-better
zuckermaninstitute.columbia.edu/science-life-daniel-du
elifesciences.org/reviewed-preprints/109793v1
Integrase anchors viral RNA to the HIV-1 capsid interior pubmed.ncbi.nlm.nih.gov/41708858/ #cryoEM
Helical polysome, maybe?
Max Fels @mfels.bsky.social from our lab discovers giant DNA viruses that infect amoeba encode eIF4E and the entire suite of 4F complex proteins to control mRNA translation, including beautiful crystal structures of viral 4E bound to modified mRNA 5' caps:
www.cell.com/cell/fulltex...
Beautiful #phage structure 🤩
Congrats to the colleagues in Brazil! 🍊🦠
www.biorxiv.org/content/10.6...
#CryoEM #xanthomonas
I had Claude make this as a mini-game for Relay's disconnect screen (like Chrome's dinosaur), but then pivoted to stand-alone. In this game, you have to find Euler angles matching the target projection, i.e. cosplay RELION. It can be pretty hard. Works on mobile too! warpem.github.io/euler-hunt/
I admit I’m completely ignorant about replication, I just have some structural-based ideas on the reason ssDNA is not working as mRNA during translation, but geometry is a likely explanation
That 2’-OH is amazing. Maybe 2’-5’ would not have the same torsional freedom as 3’-5’?
How could a simple self-replicating system emerge at the origins of life? RNA polymerase ribozymes can replicate RNA, but existing ones are so large that their self-replication seems impossible. Could they be smaller?
Excited to share our latest work in @science.org on a new small polymerase.
1/n
It took a while but it’s finally published!
The GAP activity of SEAC (GATOR) is a metabolic switch that allows cells to quickly respond to changes in amino acid levels, both for inhibition and reactivation.
Great Jan, congratulations!!
What organism? Mario Halic apparently improved Chlamy’s nucleosome to 4.2 (EMPIAR 11-830) (unofficial, I think)
Figure 1c is amazing.
Great work, congratulations
💪 NEW VIDEO:
Flying over the A-band of an atomic-scale model of a vertebrate muscle sarcomere. Let's explore the molecular mechanics that make your muscles work.
Rendered using @bradyajohnston.bsky.social 's molecular nodes
Model based on the incredible work of the @raunser-lab.bsky.social lab
Excited to share this detailed biophysical study of #PhaseSeparation in the Pyrenoid, an algal microcompartment that fixes a third of the Earth's #CO2 🌏. We combine methods across scales, in vitro 🧪 and in vivo 🦠
Thanks for the very fun collaboration! 🤗
#PlantScience 🌾 #TeamTomo 🔬 #LivingPhysics 🧬🧶
🚀 CryoSPARC v5.0 BETA is here!
We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread!
Full changelog: cryosparc.com/updates/v5.0.0
Check out our new preprint! We uncover the full molecular mechanism of rotavirus membrane penetration and cytosolic escape using cryo-ET, live-cell imaging, and single-molecule assays. (1/3)
🔗 biorxiv.org/content/10.6...
Muyuan Chen, Muchen Li, Renjie Liao: Point transformer for protein structural heterogeneity analysis using CryoEM https://arxiv.org/abs/2601.18713 https://arxiv.org/pdf/2601.18713 https://arxiv.org/html/2601.18713
LLOMe has long been used to study lysosomal damage, yet how it works has remained a mystery.
Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes – revealing a new paradigm for lysosomal failure.
🔗 doi.org/10.64898/202...
#CryoET
Gradient based refinement of CryoET tilt series alignment improves tomogram contrast and structure resolution www.biorxiv.org/content/10.64898/2026.01.16.699989v1 #cryoEM
"Use it or lose it." This is great advice for exercise, but when cells need to slow down or go dormant, they need to store ribosomes to recover growth in the future. They use hibernation factors to do this. Here is our latest story on how archaea hibernate ribosomes (1/7):
doi.org/10.64898/202...
1/10
In this thread we will explain you how we put together
#SPT #SMLM #cryoET #cryoCLEM + #modeling
to learn about the higher-order mechanism that controls exocytosis (constitutive exocytosis, the one😜)
(Illustrative animation made by the amazing Dylan Godfrey)
💡Solving decades-long mysteries with cryoEM. 💥Brilliant science + best tech = life-saving medical research. Shaping the future of health through bold new approaches.
#ParkinsonsDisease
