Zunlong Ke

I enjoyed reading the four challenging aspects you summarized. I think it’s well covered and well thought! Thank you for this review.

Figure 1. Cryo-EM of abundant protein complexes in native membranes.

Figure 2. Cryo-EM of membrane proteins in vesicles.

Figure 3. 3D reconstruction of V-ATPase in native synaptic vesicle membranes.

Figure 4. Generation of membrane vesicles for structure determination of proteins in their native lipid bilayer.

I've written a review on what I think is an extremely exciting direction in cryo-EM:

Cryo-EM of endogenous membrane proteins in their native lipid bilayer

Open access in Quarterly Reviews of Biophysics:
doi.org/10.1017/S003...

Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress Cryo–electron tomography reveals how mitochondria reorganize their translation and folding systems under proteostatic stress.

Check out this beautiful study, spearheaded by Kenny @denkenny.bsky.social, on mitochondrial remodeling. Some of the prettiest organelle segmentations I have seen 😍😍😍

www.science.org/doi/10.1126/...

Deltaviruses spread through a viral Trojan Horse Hepatitis D-like satellite viruses, known as deltaviruses, have been recently discovered in a wide range of animals. These viruses are thought to expr…

We found a viral Trojan Horse: a virus can hide inside another virus.This one surprised us: deltaviruses don’t just borrow a helper virus. They can travel inside it.
A literal Trojan Horse “virus-in-a-virus” route into cells. 🤯 Kudos to 1st author @viroscope.bsky.social and co-authors !

Amazing work from Clay @kosonocky.bsky.social, Alex Abel and LEAH labs, and all the collaborators and participants in the Bits2Binders AI CAR-T therapy protein design competition. The results are in!

bsky.app/profile/koso...

Results from an impressive world-wide binder design competition.

A bit late to post, but check out this peer-reviewed version at PNAS 2025 here: www.pnas.org/doi/10.1073/...

Postdoctoral Fellow Job Posting Title: Postdoctoral Fellow ---- Hiring Department: Molecular Biosciences ---- Position Open To: All Applicants ---- Weekly Scheduled Hours: 40 ---- FLSA Status: Exempt ---- Earliest Start ...

#PostdocRecruiting:
My group at the University of Texas at Austin is recruiting a Postdoc in virology and structural biology. Please see more details here:
utaustin.wd1.myworkdayjobs.com/UTstaff/job/...

Informal inquiries are encouraged, and see more lab info here: zunlongke-lab.org

Cryo-EM structures of prefusion HSV-2 gB in complex with human antibodies.

Our fourth installment on prefusion herpesvirus gB proteins is available as a preprint: doi.org/10.64898/202.... Working with Ivelin Georgiev's group, we isolated and characterized prefusion-specific HSV gB antibodies from humans!

Our work on HIV-1 matrix, led by James Stacey and @dhrebik.bsky.social, is in this week's @nature.com. Extended explanatory video from @margotriggi.bsky.social.

www.nature.com/articles/s41...

High-resolution in situ structures of hantavirus glycoprotein tetramers pubmed.ncbi.nlm.nih.gov/41763200/ #cryoEM

Our latest preprint is now available doi.org/10.1101/2025...! Led by my postdoc, Luqiang Guo @luqiang.bsky.social, we determined a ~2.4 A resolution structure of the hantavirus glycoprotein tetramer on the surface of VLPs. Also determined structures of dimers of tetramers and Fab-bound tetramers.

Please spread the word: the Structural Studies Division @mrclmb.bsky.social is looking for a new tenure-track, independent group leader with an exciting plan in any area of Structural (Molecular & Cell) biology, in discovery biology and/or methods development. 🥳

mrc.tal.net/vx/mobile-0/...

Congratulations Kelly! And enjoy the holiday!

Long in the making, but happy to present the Chlamydomonas chlororibosome!
Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv 📖: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n 🧵

Not something you see in textbooks very often: tripolar mitosis.

Very happy to say that our work on how axonemes are generated is now available on bioRxiv: www.biorxiv.org/content/10.6... - if you’ve ever wondered how the incredibly beautiful, complex structures within motile #cilia are constructed then this is for you!

Am I the only one increasingly annoyed by cryo-EM papers not showing *any* experimental density in figures? Sometimes not even in the supplementary material??

@erwright73.bsky.social Welcome the Wright Lab to Bluesky! #Tomo

Great work from the Wright Lab at MCCET, Madison! Congratulations to the team!

Formation & function of #MembranelessOrganelles! #CryoET structures of #proteasome storage granules inside cells!
Read our paper @cp-cell.bsky.social!

❕Publication: doi.org/10.1016/j.ce...
❕Press Release: www.biochem.mpg.de/en/pressroom

@uoftmedicine.bsky.social
@erc.europa.eu #UPSmeetMet

Check out our new preprint! We uncover the full molecular mechanism of rotavirus membrane penetration and cytosolic escape using cryo-ET, live-cell imaging, and single-molecule assays. (1/3)

🔗 biorxiv.org/content/10.6...

2026 has just begun and we are looking forward to welcoming you to EMBL for another year of groundbreaking science.

Our programme now features even more events, check it out ➡️ s.embl.org/2026-poster

Mechanism of membrane perforation in rotavirus cell entry www.biorxiv.org/content/10.64898/2026.01.21.700916v1 #cryoEM

Cryo-electron tomography reveals coupled flavivirus replication, budding and maturation - Nature Communications In this study, Dahmane et al use a method called cryo-electron tomography to uncover new details of how tick-borne flaviviruses transform cells into virus factories.

I am super happy to share this paper in its final form. We used FIB milling to "dig in to" cell lines and mouse brains infected with tick-borne flaviviruses, followed by cryo-ET to study the virus replication. It's open access, so have a look!
#virology #teamtomo
www.nature.com/articles/s41...

SHAPES being the key word, amirite @nanigrotjahn.bsky.social
github.com/GrotjahnLab/...

Should be an interesting #TeamTomo talk. Check it out! 🧪 🔬

WEBINAR: Dr. Danielle Grotjahn, Scripps Research discusses how #cryoET shapes our understanding of cellular architecture, and analyzing data to extract quantitative insights: 10 Feb, 5 PM CET | 11 Feb, 5 AM CET

Register here: ter.li/zfiv48

#CellBiology #StructuralBiology #ElectronMicroscopy

Fully genetically encoded low-molecular-weight protein tags with defined shapes for direct molecular identification by cryo-electron tomography www.biorxiv.org/content/10.64898/2026.01.16.700029v1 #cryoEM

PDB 7jsn colored by DAQ scores of fit in cryoEM map.

The ChimeraX DAQplugin computes DAQ scores showing the agreement between atomic models and cryoEM maps. Available from ChimeraX menu Tools / More Tools. cxtoolshed.rbvi.ucsf.edu/apps/chimera...

I've reviewed about 150 CVs in the past few days. A few observations: (1) I am sooo impressed by the talent pool out there; (2) do not try to make your CV fit onto one page if it makes things difficult to read - just go multipage, keeping the key messages upfront;