Nick Altemose

differential contribution of H3K9 methyltransferases to boundaries at satellites

A new and fascinating story from @bencarty.bsky.social and the group, with crucial help from the teams of @naltemose.bsky.social, Simona Giunta, and @dfachinetti.bsky.social. Many thanks to all for a fantastic collaboration.
www.nature.com/articles/s41...

Congratulations, Abby!

DNA methylation influences human centromere positioning and function - Nature Genetics Genome-wide and targeted perturbation of DNA methylation at centromeres affects CENP-A positioning and centromere structure, resulting in aneuploidy and reduced cell viability.

📢ONLINE @natgenet.nature.com

📰DNA methylation influences human centromere positioning and function.

By Catalina Salinas-Luypaert, @dfachinetti.bsky.social, @naltemose.bsky.social and colleagues.

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www.nature.com/articles/s41...

DNA methylation influences human centromere positioning and function - Nature Genetics Genome-wide and targeted perturbation of DNA methylation at centromeres affects CENP-A positioning and centromere structure, resulting in aneuploidy and reduced cell viability.

#1 Centromeres are epigenetic loci defined by CENP-A, positioned in unmethylated DNA flanked by highly methylated regions. Our work, published in @natgenet.nature.com in collaboration with @naltemose.bsky.social investigates the role of DNAme at human centromeres www.nature.com/articles/s41...

UC San Francisco, Stanford University, UC Berkeley 2025 Next Generation Faculty Symposium: A platform designed to highlight the work of exceptional early-career scientists (graduate students and postdocs) in the broad field of quantitative biological and biomedical sciences, with a track record of excellence in research, leadership, mentorship, and community engagement. Apply today! Applications due May 30th, 2025. www.berkeleystanfordnextgensymposium.com

Are you preparing to go on the biomedical faculty job market? Do you have a track record of excellence in research, leadership, mentorship, & community engagement? Apply to the 2025 Next Generation Faculty Symposium: www.berkeleystanfordnextgensymposium.com! The deadline is May 30. Please repost!

Trump’s NIH And NSF Cuts Could Cost The U.S. Economy $10 Billion Annually Economists find public R&D drives a quarter of U.S. productivity growth — and pays for itself

Federal research funding not only advances healthcare and technology, but it also reliably grows the economy. These are some of the best-spent dollars in our government! Divesting from this golden goose will sacrifice future economic growth and make us all worse off. www.forbes.com/sites/johndr...

DiMeLo-cito: a one-tube protocol for mapping protein-DNA interactions reveals CTCF bookmarking in mitosis Genome regulation relies on complex and dynamic interactions between DNA and proteins. Recently, powerful methods have emerged that leverage third-generation sequencing to map protein-DNA interactions...

I am proud to share my first preprint working with @naltemose.bsky.social, where we redesign DiMeLo-seq from the ground up. Thankful for the help and input of collaborators in @astraight.bsky.social and Aaron streets’ lab at Berkeley. More to come soon!

www.biorxiv.org/content/10.1...

DiMeLo-cito: a one-tube protocol for mapping protein-DNA interactions reveals CTCF bookmarking in mitosis Genome regulation relies on complex and dynamic interactions between DNA and proteins. Recently, powerful methods have emerged that leverage third-generation sequencing to map protein-DNA interactions...

🚨Pre-Print
Tired of losing valuable DNA-protein interaction information from your long-read seq data during wash steps?

New wash-free, one tube DiMeLo-cito protocol from @naltemose.bsky.social @astraight.bsky.social @ngamarra.bsky.social for max quality + yield!
🧪🖥️🧬 #STS
👇
doi.org/10.1101/2025...

@bencarty.bsky.social and the team did a fantastic job in a wonderful collaboration with @naltemose.bsky.social as well as Dani Fachinetti and Simona Giunta’s teams to help define the molecular basis for maintaining centromeric chromatin size and position. www.biorxiv.org/content/10.1...

Thanks, Uwe! Your class was amazing and foundational for my research career!

https://www.embl.org/about/info/course-and-conference-office/events/grg25-01/#vf-tabs__section-speakers

New conference that will explore gene regulation mechanisms at molecular resolution. We bring bring together a fine crowd that uses microscopy, genomics and theory to chart the future of the booming fields of #singlemoleculegenomics and #singlemoleculemicroscopy. Join us July 15-18th 2025 at EMBL

@arnaudkr.bsky.social and I have been talking about organizing a single-molecule genomics meeting for years! We're excited to do so at EMBL while bridging the fields of single-molecule genomics and microscopy with our co-organizers. Hope to see you there! July 15-18 www.embl.org/about/info/c...

The single-molecule accessibility landscape of newly replicated mammalian chromatin By developing a long-read sequencing method to simultaneously map replication status and protein-DNA contacts in cells, Ostrowski, Yang, et al. show that newly replicated chromatin is enriched for unw...

The latest from our group, led by Megan Ostrowski and @martyyang.bsky.social, is now published in final form (www.cell.com/cell/fulltex...! Many thanks to our excellent peer reviewers for suggesting several experiments (including CAF-1 perturbation) to really improve the study =) #epigenetics