Ivano Legnini

Had a great day at Human Technopole - lots of stimulating discussions with students and faculty and also, this should be a new standard, Aperol spritz after the seminar! @humantechnopole.bsky.social

Still a few days to apply :)

Single-cell and isoform-specific translational profiling of the mouse brain - Nature Post-transcriptional regulation of mRNA translation was explored using Ribo-STAMP and single-cell RNA sequencing to reveal cell-type-specific and isoform-specific translation patterns across hippocampal neuronal and non-neuronal cell types, highlighting functional differences between CA1 and CA3.

Nature research paper: Single-cell and isoform-specific translational profiling of the mouse brain

go.nature.com/4aYPQu7

Thrilled to announce that I’ve just opened my research lab at @helmholtz-hiri.bsky.social !
A huge thank you to my mentor Roi Avraham (and lab members!) for the incredible training and support that made this possible.

6/6 Bonus credits: all previous work on this is by our amazing lab chancellor @azappulo.bsky.social, who will co-supervise this project

5/6 Join us at HT, a vibrant international research institute, located in beautiful Milan (food, culture and the nature around are definitely a plus): humantechnopole.it/en/

Ciliopathies - Human Technopole

4/6 we now want to use these models to study neural tube defects, with exciting internal and external collaborations, such as the @gaiapigino.bsky.social group within the Flagship Research Program on Ciliopathies: humantechnopole.it/en/research/...

3/6 We developed new organoid models for studying neural tube patterning, where we use optogenetics to locally perturb morphogen expression

2/6 If you're passionate about neurodevelopment, organoids, single-cell and spatial omics, please apply here:
careers.humantechnopole.it/job/Postdoc-...

Legnini Group - Human Technopole Laboratory for Molecular and Systems Biology of RNA   The Legnini Group at Human Technopole combines molecular and systems biology approaches to study gene regulation. We use synthetic biology and opt...

1/6 We're hiring! Fully funded postdoc position in my group
humantechnopole.it/en/research-...

Exosome secretion is required for sonic hedgehog dispersal and signal gradient formation in the embryonic limb mesenchyme Carrier-assisted diffusion and cytoneme transport have been postulated to disperse Hedgehog across diverse embryonic tissues, yet their relative contributions to patterning mesenchymal tissues remains...

SHH exosomes surfing along the OUTSIDE of mesenchymal cell cytonemes - check it out!

www.biorxiv.org/content/10.6...

Modelling co-development between the somites and neural tube in human trunk-like structures - Nature Cell Biology Makwana, Tilley et al. generate human stem cell-based trunk-like structures approximating Carnegie stage 13–14 of development. They use them to model and study the development of the thoracic and lumbar trunk.

Lovely little pre-Christmas present to see this out @natcellbio.nature.com! Some 🔥 new results in here since the biorvix incl (1) a new RARE-GFP reporter ✳️🙌, (2) additional NMP quantification 🔢, (3) no neural tube patterning on RA inhibition 🙅 etc. Enjoy! 😍 www.nature.com/articles/s41...

Investigating morphogen and patterning dynamics with optogenetic control of morphogen production Benzinger et al. develop a chemo-optogenetic system for precise spatiotemporal control of morphogen production in vitro. Using light-controlled Sonic hedgehog expression, they recapitulate neural tube...

Good to see the peer reviewed version of this published

A chemo-optogenetic system for spatiotemporal control of gene expression

We use it to:

- Reconstruct SHH mediated neural tube patterning in vitro

- Measure extracellular half-life of Shh

www.cell.com/developmenta...

😵‍💫

Still a couple of weeks to apply for a fully funded PhD position in #AI and #RNA biology!!!

🔬 Join Human Technopole!

🧠 Our @ilegnini.bsky.social Group seeks a #Postdoc in Molecular Systems Biology to study genetic–environmental interactions in early neurodevelopment.

Apply by 30 Nov 2025 👉 careers.humantechnopole.it/job/Postdoc-...

And in addition to the postdoc position (1 week left to apply!), just opened a PhD position in AI and RNA biology 🤩

PhD position in my lab in #AI #RNA, in co-supervision with superstar @florianjug.bsky.social

www.polimi.it/dottorato/fu...

One more week to apply 🐣

🥹

Bonus 2. Apparently we do wonderful lab retreats, and the Alps are a stone's throw away 🧡

Bonus credits: all work in thread is by our incredible lab chancellor @azappulo.bsky.social, who will co-supervise this project

6/6 located in beautiful Milan, incredibly exciting city with its amazing culture and food scene, and wonderful nature around. Write me if you're interested or for any questions, and please repost ❤️

Human Technopole Human Technopole is Italy’s new research institute for life sciences that aims to increase public and private investments towards scientific research and to be actively involved in the promotion of sc...

5/6 Join our group @humantechnopole.bsky.social, a vibrant and international environment humantechnopole.it/en/

Pigino Group - Human Technopole Cells need to be able to sense different types of signals, such as chemical and mechanical signals, from the extracellular environment to properly function. Most eukaryotic cells perform these functio...

4/6 we now want to use these models to study neural tube defects, with exciting internal and external collaborations, including the Pigino group humantechnopole.it/en/research-...

3/6 We developed new organoid models for studying neural tube patterning, where we use optogenetics to locally perturb morphogen expression

2/6 If you're passionate about neurodevelopment, organoids, single-cell and spatial omics, please apply here: careers.humantechnopole.it/job/Postdoc-...

Legnini Group - Human Technopole Laboratory for Molecular and Systems Biology of RNA   The Legnini Group at Human Technopole combines molecular and systems biology approaches to study gene regulation. We use synthetic biology and opt...

1/6 We are hiring!!! 🐣🐣🐣
Fully funded postdoc position in my group!
humantechnopole.it/en/research-...

Legnini Group - Human Technopole Laboratory for Molecular and Systems Biology of RNA   The Legnini Group at Human Technopole combines molecular and systems biology approaches to study gene regulation. We use synthetic biology and opt...

Looking for a master student to help with a superexciting EU-funded project on mRNA metabolism. It'll involve cutting-edge sequencing and imaging techniques as well as computational workflows and modelling - great training opportunity! Get in touch if interested
humantechnopole.it/en/research-...

Immune responses need to be fast ⏱️ mRNA metabolism adapts rapidly via alternative splicing, polyadenylation & decay. Find out more: febs.onlinelibrary.wiley.com/doi/10.1002/...
#Immunology #RNA #mRNAmetabolism @ilegnini.bsky.social