@tanner-fadero
(he/him) Light microscopy scientist interested in developing and deploying better live-cell fluorescence microscopy technology. Tweets about microscopy🔬, cell biology, and optics. Conflicts of interest: http://tinyurl.com/y25ctubo
@patrickzager.bsky.social acquired this lovely 3D timelapse of live neutrophil membrane protrusions on the snoutscope at the UCSF Center for Advanced Light Microscopy. Shown are three orthogonal maximum intensity projections.
But I'd really recommend getting one of @jsdaniel02.bsky.social's passive thermal crossbraces for O2/O3! No need for a piezo to actively correct for thermal drift of O2/O3 when the thermal crossbrace will passively refocus for you!
Rotating the frame of reference to agree with microscope 3 and adding a camera plane (brown) makes the lateral motion of the spot's image more obvious.
See quick simulation here. From top to bottom:
- point source at microscope 2's image plane
- TL2
- O2
- O3
- TL3
I translate O3 along its optical axis. Note the apparent left/right motion of the image of the spot at TL3's focal plane
I second the thermal crossbrace over any active focusing mechanism.
Also, O2's axis is tilted w.r.t. O3's optical axis. So an axial motion of O3 would result in a lateral apparent motion of O2's focal point.
Integrated in an imaging system with a 60X NA 1.49 lens.
Pretty clean rotation near optical axis.
There are some things that blue lasers can't excite...
... for everything else, there's red and orange lasers
Not just fluorescent proteins; they developed an ANTIBODY that changes its binding in response to a magnetic field!
bsky.app/profile/andr...
Researchers have engineered magnetically controlled fluorescent proteins that can be remotely dimmed and brightened in cells and living animals
go.nature.com/3NUSY12
Great news! @mariaingaramo.bsky.social 's company (Nonfiction Labs) made a remote-controlled antibody.
Its binding turns on and off with a magnet.
This is a HUGE step towards our dream of magnetically controlled drugs. Imagine a cancer drug that ONLY attacks the tumor, not the rest of your body.
We’re thrilled to feature Dr. Tanner Fadero from the Chan Zuckerberg Imaging Institute in an upcoming Nature-hosted webinar about... PHOTONS! 😎 Join the webinar to learn and take part in the live Q&A. 👉 Register here: https://ow.ly/vsyn50XkZ88
#FluorescenceMicroscopy #LightSheetMicroscopy
Good point! I've yet to find one with an absolute positional encoder that doesn't need to home. This probably isn't the exception.
The DC servo (Z912B) says it is encoded with 34555 counts/mm
www.thorlabs.com/thorproduct....
Motorized slit! Off-the-shelf part from ScienceTech. Thorlabs stepper motor driven by K-cube controller.
www.sciencetech-inc.com/shop/product...
@amsikking.bsky.social @retof.bsky.social @kevin-dean.bsky.social
Playing around with how to represent volume in #Snouty light sheet data. Here is a dividing hiPSC with endogenously tagged histone in all three orthogonal projections. Bluer is further away. If anything, it distracts from politics.
Yes, there's a nice trick to compensating for transferring possession to the table from the crane. But there's also certain steps you can take before giving possession to the crane that decrease tension, leading to less severe shifts during future transfers. I'd love to walk you through them!
the anticipation is killing me
(I see nothing)
@jsdaniel02.bsky.social can make it happen!
@tanner-fadero.bsky.social told me there was a discussion about the unofficial name for #snouty V3. We have had Mr. Snouty and King Snouty, and there were people suggesting Emperor Snouty. I think it is time for a Lady or EMPRESS snouty. I have created Art to support this.
What if we could use 'any' immersion objective and get good 3D imaging? Normally we can't as we must refractive index match the immersion to the sample (previous post).
-> However, I realized a few years ago that we can get good 3D imaging with any immersion using remote refocus optics!
But yeah, a modest tilt angle between projections looks similar!
Oh neat! You're looking at a projection from the "native" angle. Interestingly, the closer your sheet tilt angle is to 0°, the more similar the native projection is to the normal Z projection.
If that projection is from the deskew plug-in, then it's a true max intensity Z projection (where Z is O1's optical axis).
The deskew plug-in deskews AND rotates your data.
Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.
Bluesky is now the platform of choice for the scientific community.
From @jenlucpiquant.bsky.social on @arstechnica.com
arstechnica.com/science/2025...
