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@sevaplasmids

A non-profit Open Access repository of standardised plasmids following the SEVA format. Check out our database and request your #plasmid free of charge #synbio seva-plasmids.com

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22.11.2024
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Latest posts by @sevaplasmids

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Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

⭕✨Standard, tailor-made, and send directly to your door food free. Too good can be true. Take a look at our website and check our vectors free of charge 👉

08.03.2026 23:00 👍 0 🔁 0 💬 0 📌 0

🦠✨This bacterium can act as a “sickness traffic light”. @ctropini.bsky.social and her group from the University of British Columbia have engineered a system in which cells that normally produce GFP reduce their fluorescence under stress conditions.
Discover the full story 👉https://shorturl.at/f9bpu

04.03.2026 23:01 👍 0 🔁 0 💬 0 📌 0

❓🦠With N, what is the main cause of foodborne illness? Non-typhoidal Salmonella! In it, plasmids enhance adaptability, combining resistance and virulence. Microbiota-targeted or CRISPR-based tools can provide opportunities to disrupt plasmid dissemination. 👉Check how:

03.03.2026 11:00 👍 0 🔁 0 💬 0 📌 0
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Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

Science anywhere? Absolutely yes! Science anyhow? No please! The #SEVA database of vector plasmids aims to improve Science replication and create a world in which high-quality science is available no matter where. Join us! 👉 seva-plasmids.com

26.02.2026 11:01 👍 1 🔁 0 💬 0 📌 0
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A global soil plasmidome resource unveils functional and ecological roles of plasmids in soil microbiomes - Nature Communications Here, the authors report the Global Soil Plasmidome Resource, a comprehensive plasmid soil catalog, that explores the roles of plasmids in microbial adaptation and host integration, highlighting…

The @berkeleylab.lbl.gov presented the Global Soil Plasmidome Resource. A comprehensive dataset of 98,728 plasmid sequences amassed from 6860 terrestrial microbial communities and isolates. Check them out!👉 www.nature.com/articles/s41...

24.02.2026 11:01 👍 1 🔁 0 💬 0 📌 0
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FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…

Replicable science requires shared standards. The #SEVA platform provides modular, standardized plasmid vectors that improve reproducibility and design. Explore the SEVA collection and order validated plasmids for free 👉 seva-plasmids.com/find-your-pl...

23.02.2026 11:01 👍 1 🔁 0 💬 0 📌 0
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Beyond borders: plasmids drive a shared antibiotic resistome in European urban water systems - Microbiome Background Urban wastewater systems (UWSs) act as reservoirs and conduits for the dissemination of antibiotic resistance genes (ARGs), with plasmids playing a central role in their spread. Despite…

🌍🧬 Plasmids are spreading antibiotic resistance across European urban water systems.
A multi-omics survey found tens of thousands of plasmids carrying resistance genes, many previously unknown. It highlights how wastewater networks share and mobilize ARGs across borders 👉https://doi.org/qn2v

20.02.2026 11:01 👍 2 🔁 0 💬 0 📌 0
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Comparative analysis of plasmid vectors used in gene editing and genetic engineering Plasmid vectors are foundational tools in gene editing and genetic engineering, enabling targeted genome modification, heterologous gene expression, and metabolic pathway optimization across diverse…

🧬📊 Read this comparative review of plasmid vectors used in gene editing and genetic engineering. It highlights how designing elements such as replication origin, host range, and CRISPR compatibility affect multiple features. How important it is to make the right selection? 👉

18.02.2026 11:01 👍 0 🔁 0 💬 0 📌 0
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FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…

If you are a researcher working with plasmids, this is for you!👩🏾‍🔬🔬Standard, effective and ✨free✨, what else can you ask for? Take a look at our website and find your #SEVAplasmid ready to use 👉

16.02.2026 11:01 👍 0 🔁 0 💬 0 📌 0
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A Versatile Plasmid System for Translational Control and Secretion of Recombinant Proteins in Mycobacteria Recombinant protein expression in mycobacteria faces two major challenges: limited regulatory tools for inducible expression and inefficient secretion of heterologous products. In this study, we…

🧬🔧 New tools keep emerging for mycobacterial protein production. This plasmid-based system enables translationally gated secretion in Mycobacterium, combining riboswitch control with highly efficient extracellular export. A new layer of regulation unlocked👉

14.02.2026 11:01 👍 2 🔁 0 💬 0 📌 0
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Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

A free, worldwide and standardized tool for researchers 🧑‍🔬🛠️ It seems too good to be true, but it exists! ⭕Our #SEVA plasmids accomplish all those characteristics to help scientific research succeed. Use them yourself! 👉

12.02.2026 11:01 👍 1 🔁 0 💬 0 📌 0
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A therapeutic glue that autonomously targets gut bleeding in inflammatory bowel disease - Nature Biotechnology We engineered bacteria to function as therapeutic ‘living glues’ that autonomously detect gastrointestinal bleeding and, in response, secrete adhesive and therapeutic proteins to enable targeted…

🩸🩹You may have heard about using glue to stop bleedings. Maybe it is not the best strategy in the world, but scientists have engineered bacteria to function as therapeutic ‘living glues’ inside your body! 😳. Check how they do it 👉

10.02.2026 11:01 👍 1 🔁 0 💬 0 📌 0
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Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost Abstract. Plasmids promote adaptation of bacteria by facilitating horizontal transfer of diverse genes, notably those conferring antibiotic resistance. Som

⭕ Plasmids of the group lncP-1 replicate and usually persist in many bacteria, but nothing in nature comes without exception. The lncP-1𝛃 plasmid pBP136 became extinct in E.coli at the lab. Scientists from the @uidaho.bsky.social suspect which gene is guilty. 👉

06.02.2026 11:00 👍 0 🔁 0 💬 0 📌 0

🧬Plasmids are the workhorses of molecular biology, but are we really choosing them wisely? @vdlorenzo.bsky.social and Esteban Martínez-García revisit what really defines plasmid performance and why modular standards like #SEVA matter for the future of #SynBio 👉https://doi.org/10.1111/1751-7915.70273

04.02.2026 11:01 👍 1 🔁 1 💬 0 📌 0
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CONTACT - SEVA plasmids - Standard European Vector Architecture Order plasmids If you wish to order SEVA plasmids, please fill in the form below. We offer this service pro bono so it is free of charge. Large orders ( > 15 plasmids) may experience shipment delays…

Have you already found your pSEVA plasmid match for your #SynBio project? You’re just one step away! 🚀

📤 Visit our website and choose the gateway to your next experiment—free, open, and ready to use 👉https://seva-plasmids.com/contact/

02.02.2026 11:01 👍 0 🔁 0 💬 0 📌 0
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Engineering microbial consortia for mixed plastic upcycling - Nature Communications The chemical complexity of post-consumer ‘mixed’ plastic waste limits its use as a feedstock for biomanufacturing. Here the authors combine transition-metal-free plastic deconstruction with a…

♻️🦠Tackling mixed plastics! See the potential of engineered microbial consortia to upcycle complex mixed plastic waste. Integrating transition metal-free plastic depolymerization with the strengths of each bacterial specialist and division of labor 👇
doi.org/10.1038/s414...

28.01.2026 11:01 👍 2 🔁 0 💬 0 📌 0
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Description - SEVA plasmids - Standard European Vector Architecture What is the SEVA format? The SEVA format involves a set of rules for the physical assembly of the three basic components of plasmid vectors (origin of replication, selection marker and the business…

In need of a new #SEVA construct? Contributing to the community and to the plasmid repository is simple:

1. Remove unwanted and extra restriction sites
2. Flank every module with the appropriate SEVA-standard sites
3. Send your construct!

📋Check all the details seva-plasmids.com/description/...

23.01.2026 13:01 👍 0 🔁 0 💬 0 📌 0
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A synthetic cell phage cycle - Nature Communications The reconstitution of complex biological processes in cell-free systems can support the detailed characterisation of biochemical mechanisms which are difficult to probe in vivo. Here authors present…

🔬Investigating viral infections in a controlled way: researchers establish a cell-free T7 phage cycle on synthetic cells. A simplified but promising in vitro system for studying viral infection variables, phage-host interactions, and more. Full story👇
doi.org/10.1038/s414...

22.01.2026 13:00 👍 2 🔁 0 💬 0 📌 0
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Broad-Host-Range Synthetic Biology: Rethinking Microbial Chassis as a Design Variable Broad-host-range synthetic microbiology is redefining the role of microbial hosts in genetic design by moving beyond the traditional organisms. Historically, synthetic biology has focused on…

🦠⏩Beyond traditional organisms, broad-host-range synthetic biology offers a powerful framework for innovation, expanding chassis selection and rethinking their role in genetic design. Take a look at the perspective paper doi.org/10.1021/acss...

20.01.2026 13:30 👍 1 🔁 0 💬 0 📌 0
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FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…

Let’s break down a pSEVA plasmid’s mix-and-match available modules!
🔵 Antibiotic resistance marker
🟡 Origin of replication
🔴 Cargo
🟢 and optional gadget

Check our user-friendly platform and find the plasmids with the ideal modules for your research! 👉 seva-plasmids.com/find-your-pl...

15.01.2026 13:30 👍 1 🔁 0 💬 0 📌 0
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Intracellular competition shapes plasmid population dynamics From populations of multicellular organisms to selfish genetic elements, conflicts between levels of biological organization are central to evolution. Plasmids are extrachromosomal, self-replicating…

🧬How selective pressures shape plasmid evolution within the host cell?

Scientists have measured within-cell fitness of competing plasmids, revealing new insights into cell and plasmid dynamics and into how antibiotic resistance evolves in bacteria! Take a deeper look doi.org/10.1126/scie...

14.01.2026 13:30 👍 0 🔁 0 💬 0 📌 0
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citrOgen: a synthesis-free polysaccharide and protein antigen-presentation to antibody-induction platform - Nature Communications Bacterial antigens, such as lipopolysaccharides, are complex structures which remain difficult to synthesise or purify for antibody generation. Here, authors present a platform technology using…

👩‍🔬Researchers use a SEVA plasmid to help develop citrOgen. A rapid platform that repurposes Citrobacter rodentium to present antigens from high-priority human pathogens, triggering the host to produce protective antibodies🛡️.

👉https://doi.org/10.1038/s41467-025-63922-0

09.01.2026 13:01 👍 1 🔁 0 💬 0 📌 0

A standardised plasmid repository for experimental replicability ⭕. Check #SEVAplasmids database and join the movement!
👉 seva-plasmids.com/find-your-pl...

07.01.2026 13:02 👍 0 🔁 0 💬 0 📌 0

✨And with that the 2025 season comes to an end...
But at SEVA we continue working for a replicable and reliable science🤝 We want to thank you all for your support through this past year, and we can’t wait for all the surprises 2026 will bring! 🤩

31.12.2025 11:00 👍 2 🔁 1 💬 0 📌 0
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Self-documenting plasmids Plasmids are the workhorse of biotechnology. These small DNA molecules are used to produce recombinant proteins and to engineer living organisms. They…

📋Your plasmid and your plasmid documentation all in one place! Have a look at the possibility of plasmids with self-encoded documentation files 👉 doi.org/10.1016/j.ti...

29.12.2025 11:00 👍 0 🔁 0 💬 0 📌 0
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Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

👩‍🔬Can’t find your plasmid? We’ve got you covered! Design your own vector looking for the different pieces to assemble it yourself. All for free 👉 seva-plasmids.com

26.12.2025 11:00 👍 0 🔁 0 💬 0 📌 0
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Establishing Halomonas as a chassis for industrial biotechnology: advances in synthetic biology tool development and metabolic engineering strategies - Microbial Cell Factories Background Halomonas species have recently emerged as promising chassis organisms for next-generation industrial biotechnology, due to their ability to thrive under high-salt conditions, where most…

From a non-model organism to a promising industrial chassis✨. Have a look at the developments on synthetic biology tools and the engineering potential of Halomonas. SEVA plasmids have been used in some of these efforts, check the review 👉 doi.org/10.1186/s129...

23.12.2025 11:00 👍 1 🔁 0 💬 0 📌 0
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COLLABORATORS & RESOURCES - SEVA plasmids - Standard European Vector Architecture The SEVA commonwealth Laboratory of Dr. Sven Panke. Bioprocess laboratory. ETH. Switzerland. Laboratory of Dr. Nediljko Budisa.

🤝Professional labs around the globe are expanding SEVA and its standard. Meet our collaborators and learn about their research!
seva-plasmids.com/the-seva-com...

18.12.2025 13:30 👍 0 🔁 0 💬 0 📌 0
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Genetic controllers for enhancing the evolutionary longevity of synthetic gene circuits in bacteria - Nature Communications Engineered gene circuits often degrade over time due to mutation and selection. Here the authors use a host-aware modelling framework to develop genetic controllers to sustain synthetic gene…

👀 Researchers at University of Warwick explore how to maintain the expression of synthetic gene circuits over time in bacteria. They propose three genetic controllers that improve simple circuit half-life over threefold. Take a look 👉 doi.org/10.1038/s414...

17.12.2025 13:00 👍 3 🔁 0 💬 0 📌 0
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Construction of antibiotic-free riboflavin producer in Escherichia coli by metabolic engineering strategies with a plasmid stabilization system Riboflavin, an important vitamin utilized in pharmaceutical products and as a feed additive, is mainly produced by metabolically engineered bacterial …

A plasmid stabilization system offers a way to reduce antibiotic reliance in riboflavin bacterial production. Learn more about the solution 👇 👇
doi.org/10.1016/j.sy...

12.12.2025 13:00 👍 0 🔁 0 💬 0 📌 0