New paper from my team detailing a greatly expanded genomic database of Asgard archaea revealing of high energy metabolism those related to eukaryotes! Led by @katyappler.bsky.social lots of help from @jameslingford.bsky.social @valdeanda.bsky.social @kassipan.bsky.social doi.org/10.1038/s415...
A π #Ichthyosporea story
Led by@margaridaaraujo.bsky.social, in Co. @gautamdey.bsky.social & @hiralshah.bsky.social+π₯team across @embl.org & @sciencesunige.bsky.social
We finally have a look at what #microtubules are doing during #cellularization in #Sphaeroforma
βΆοΈ tinyurl.com/MTSARC
#ProtistsOnSky
When all the female grad students tell you a candidate seems arrogant & smarmy & they donβt like him, but itβs all vibes, maybe hire one of the other candidates who are just as amazing researchers, but donβt give off bad vibes. Cuz in my experience those vibes have proven right 100% of the time.
There are far more talented candidates than there are tenure-track jobs. Mentoring students is a major component of a faculty position. It is entirely possible to pick talented candidates who arenβt weird assholes, it just requires hiring committees to value interpersonal skills.
Long in the making, but happy to present the Chlamydomonas chlororibosome!
Cryo-ETπ¬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv π: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n π§΅
For #ECRβs:
DBGβs Hot Tpoic Workshop
on Cryo-Electron Tomography + FIB-milling
13 β 15 April 2026
In @cellarchlab.com 's lab @biozentrum.unibas.ch
Organised by @tamb-o.bsky.social, Karen Zinzius, @fannyleblanc.bsky.social
Details: www.deutsche-botanische-gesellschaft.de/en/about-us/...
You like #LLPS? #TeamTomo? #Rubisco? Check out our latest preprint!
What a great collaboration this has been!
Excited to share this detailed biophysical study of #PhaseSeparation in the Pyrenoid, an algal microcompartment that fixes a third of the Earth's #CO2 π. We combine methods across scales, in vitro π§ͺ and in vivo π¦
Thanks for the very fun collaboration! π€
#PlantScience πΎ #TeamTomo π¬ #LivingPhysics π§¬π§Ά
Check out this cool thread on the latest preprint by Gaurav Kumar, @james-r-barrett.bsky.social, @phaips.vd.st from our lab and colleagues, dissecting the physics of Rubisco condensates!
You're in for some stunning #cryoET of the pyrenoid - in Chlamy, of course π
Congrats to all involved!
How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algaeβthe pyrenoidβto find outβ¦ π§΅
Preprint here!:
www.biorxiv.org/content/10.6...
@cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social
#Rubisco #PhaseSeparation #Condensates #Pyrenoid
π¬π¨New preprint alert! π¨π¬
We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology
www.biorxiv.org/content/10.6...
#SuperResolution #CellBiology
π’ Open faculty position β Origins of Life
We have an opening in our section at the University of Geneva! π§¬π
SPREAD THE WORD
Apply here: jobs.unige.ch/www/wd_porta...
The latest issue is now online www.cell.com/molecular-ce...
Happy to see this out π₯³
authors.elsevier.com/sd/article/S...
#TeamTomo #CryoET #CryoEM
If you want to learn how to process cryo-ET data using the Chlamy dataset don't forget to check : tomoguide.github.io
@phaips.vd.st
Come join us! Soon I will be advertising a postdoc vacancy in my group as part of my @erc.europa.eu AdG project 'DARK ROOTS'. Focus of the project will be on phylo- and metagenomic mining of novel prokaryotic lineages. I will soon post a link here - stay tuned, and please repost! #asgardarchaea
Starting at 7pm Central I will be streaming from the bottom of the ocean off the coast of Uruguay!
Feel free to join and ask questions.
www.youtube.com/@SchmidtOcean
An Asgard archaeon with internal membrane compartments
Brilliant study led by @fmacleod.bsky.social and Andriko von KΓΌgelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Very happy that NWO supports this major upgrade of our national EM infrastructure. It is great that this initiative got broad support all over NL, and it will EMPower fantastic science - looking forward to that!
www.uu.nl/en/news/33-m...
Thrilled, and admittedly relieved, to see this major investment in Dutch #cryoEM become reality! π³π±π¬
Honoured to be a co-applicant on this national initiative and very excited for the opportunity to advance our work in cellular structural virology! π¦
www.uu.nl/en/news/33-m...
Weβre back with the next DinoSphere Online Seminar!
Join us on Nov 4th, we're hosting:
EdmΓ©e Royen (ULiΓ¨ge) - Symbiodiniumβ¨
Nicolas dos Santos Pacheco (Cambridge) - Perkinsids
π
Tue, Nov 4 - 4 PM CET/3 PM GMT
β¨π» Zoom link: sites.google.com/view/dinosphereβ¨
Please spread the word!
#protistonsky
Fig. 2.Confocal microscopy of N. punctiforme strains. Filaments from the indicated strains were taken from cultures grown in BG110 medium (not induced; A) or after incubation for 24β h in the presence of Anthoceros agrestis exudates (hormogonia induction; B) and visualized by confocal microscopy. Merged (2 channels) show the overlay of autofluorescence (magenta) and GFP fluorescence (green). Merged (3 channels) represent the overlay of magenta autofluorescence, bright field, and green GFP fluorescence. Hormogonia are identified as short filaments with small cells. Scale bar=20β ΞΌm.
π‘π¬ TECHNICAL INNOVATION π‘π¬
Neubauer et al. developed a Nostoc reporter strain enabling high-throughput quantitative monitoring of hormogonia differentiation in cyanobacteria as a response to biotic and abiotic factors.
π doi.org/10.1093/jxb/...
#PlantScience π§ͺ @peterszovenyi.bsky.social
π₯¨π¬!
π± Using βcompellingβ methods, including #CryoET, researchers mapped spinach thylakoid membranes at single-molecule precision, revealing how photosynthetic complexes are organised and settling long-standing debates on chloroplast architecture.
buff.ly/j3TSIkn
New short paper from our lab @currentbiology.bsky.social, in which we discover of a new mode of cell motility for choanoflagellates: flagellar gliding. www.cell.com/current-biol... - A π§΅
A new #UExM probe for #Actin!
Fun times thinking/developing/testing with @centriolelab.bsky.social & @lreymond.bsky.social !
Congrats to all !
If interested in #UExM, or #HAK-Actin, feel free to reach out !
Soon available at @spirochrome.com
#Expansion #Microscopy #ProtistsOnSky
Our new preprint with a new #Asgard from stromatolites (and its Desulfobacterota companion). Complete circularised genomes of both, cool #cryoem images, protein modelling, etc
Big group effort with a lot of people involved
#archaeasky #archaea
www.biorxiv.org/content/10.1...
Please Re-Post. Our global GRUMP microbial ocean database is out in Scientific Data! Unfractionated, single universal PCR, with Archaea, Bacteria, and Eukaryotes all on the same scale with the same denominator. Pole to pole, depths to 6000m. Lots of metadata. www.nature.com/articles/s41...
After 5 amazing years @pilhoferlab.bsky.social I'm starting my lab @embl.org Grenoble this November embl.org/wollweber
We'll image Asgard archaea and many other strange microbes ( #archaeasky, #protistsonsky..) to understand eukaryogenesis
First job ad: #teamtomo scientist!π¬βοΈ tinyurl.com/2rdu2ze6
Excited to share the lab's 1rst preprint! Rapid high-res immunofluorescence is now possible in cultured & environmental diatoms thanks to 4-fold expansion microscopy. A step-change for comparative cell biology in one of the most important phytoplankton groups on the planet π₯³
tinyurl.com/m9s5su7s 1/2
