📢 Recrutement d’un(e) Maître(sses) de conférence en Biochimie générale, spécialité bactériologie moléculaire à MMSB

🔬Recherche
*Immunité anti-phages
*Réponse au stress, modulation de la croissance bactérienne
✉️ christophe.grangeasse@cnrs.fr

📚Enseignement : Université Lyon I
✉️ patrice.gouet@ibcp.fr

Hi Oli,
FYI I advised a colleague to test your procedure on his protein and it deblocked the structure solving for him! It's a small protein also.
Great that you found this trick.

Just saw a talk about regulation of a Kir channel by a small RNA as well. But the mechanism of regulation is not clear.
Definitely not the two types of macromolecule we'd expect to interact...

All travelers from ESTA countries (yes the ones on visa waiver programs) will have to disclose 5 years of social media + huge amounts of personal data to enter the US now. All US academic associations should now meet outside the US if we want to meet our international colleagues.

Overall resolution is not the good metric to compare xray and cryo, but my guess is that local resolution cryo and overall xray are similar when you want to see holes in aromatics.
At these resolutions, diffraction is mostly isotropic and treated as overall for the whole dataset, but not cryo.

Discovery of new AMPA receptor regulators – RNA molecules that act as competitive antagonists causing CNS-related toxicity when intended for treatment of autosomal dominant polycystic kidney disease (ADPKD). Collaboration with Regulus Therapeutic/Novartis! www.nature.com/articles/s41...

Holy shit.

AQuaRef: machine learning accelerated quantum refinement of protein structures - Nature Communications AQuaRef employs machine learning to refine protein structures from cryo-EM and X-ray data in Phenix. It achieves quantum-level precision, improving model geometry and fit to the data while reducing ov...

Finally out in print! Long time project with @olexandr.bsky.social and the PHENIX group. Refinement of X-ray and CryoEM structures, using Machine Learned Potentials as the back end to help fitting. This fulfills the old dream of doing QM refinement, but very very cheap www.nature.com/articles/s41...

Looking very promising, it's a great idea. Can't wait to test it.

Very clever!

Thanks for sharing Oli.
FYI I tried it on a larger membrane protein for which the 2D classes were not high res (not enough particles) but I hoped for a miracle. The miracle didn't come, I ended with a high res bad model, a negative control I guess.
I'll nevertheless keep the preprint in my folder

Soon... a new building for our laboratory, with 1400m² extra and renovated space to host new equipements such as cryoEM, CryoFIB-SEM, optical microscopies in biosafe confined environment, and a synthetic biology labs.

This one is a bit of a departure from the usual and definitely a work in progress!

We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.

Read on for details... 1/x

Sub-3 Å resolution protein structure determination by single-particle cryo-EM at 100 keV Cryoelectron microscopy (cryo-EM) has transformed structural biology by providing high-resolution insights into biological macromolecules. We report s…

Now out in Structure: Paper on high-resolution #cryoEM using the 100 kV Tundra cryo-TEM by Dimple Karia, Adrian Koh, @abhaykot.bsky.social, @lingboyu.bsky.social, @landerlab.bsky.social and many others. Happy to have contributed to this effort. Check it out:
www.sciencedirect.com/science/arti...

AlphaFold developer Google DeepMind to fund CASP as NIH funding falls short Protein structure prediction contest CASP gets temporary funding from Google DeepMind as NIH grant runs out.

NEW from me at STAT:

We've learned that DeepMind is going to fund CASP, the protein structure competition that brought Google DeepMind's #AlphaFold to prominence (and a Nobel Prize), as its NIH funding runs out.

More @statnews.com:
www.statnews.com/2025/07/21/c...

🖥️🩺🧪🧬 #bioML

Tucked away in the 'One Big Beautiful Bill' is the requirement that all visitors to the US must pay $250 just to enter the country starting 1 October! For a family of 5 that's $1250! I doubt this will help America's crashing tourism business which is down 10% already since January

Thank you to our laureates who work everyday to make science more sustainable.
Many small steps that can make a difference.
Why not do them?
What's your action today?

A great stimulating place to work and collaborate.

Following court order, NIH ceases new terminations of politically sensitive grants After a judge told agency to restore hundreds of killed grants, NIH backs off plans to cancel more existing projects

Breaking news: The National Institutes of Health has ceased terminating grants on politically sensitive topics after a federal judge ruled last week that the cancellations were illegal.
scim.ag/4l9MO8Y

Looking for a deep dive into 2D classification in #cryoEM?

These recent guide pages cover practical aspects & parameter choices when running these jobs in #CryoSPARC, along with the theory behind expectation maximization: guide.cryosparc.com/processing-d... and guide.cryosparc.com/expectation-...

New work from our lab. Structures of K2P6.1 (TWIK2) that yield a new and suprising perspective on how lipids affect K2P channels. Great work from Abishek Mondal and Sangeeta Niranjan in my lab.

www.biorxiv.org/content/10.1...

Congratulations to Lauriane @mmsb-lyon.bsky.social and all the group for this splendid work

Rhodamine6G and Hœchst33342 narrow BmrA conformational spectrum for a more efficient use of ATP - Nature Communications ABC transporters are mobile and adaptative molecular machines. Here, Gobet et al characterize how substrate-binding influences the ABC transporter BmrA and triggers cooperativity in ATP-binding, utili...

🎉 Congratulations to Alexia from the Drug Resistance & Membrane Proteins Group at MMSB and her coworkers for being awarded the 'Article of the Month-May 2025' by the @sfbbm.bsky.social for their publication!

The SFBBM highlight is available here:
www.sfbbm.fr/index.php?op...

Junior Group Leader Position in Computational Structural Biology - Marseille (Arrondissement), Bouches-du-Rhône (FR) job with AFMB Laboratory (amU/CNRS/INSERM/INRAe) | 12839733 Junior Group Leader position with a focus on applying artificial intelligence (AI) to the field of integrative structural biology.

We are recruiting a junior group leader in computational structural biology.
Spread the news!

www.nature.com/naturecareer...

Hey #CryoEM and #TeamTomo world— check this powerful tool for optimizing your maps! 🧪🧶🧬🔬

Wow! Great structures

We are happy to release LocScale2.0: a tool for context-aware, confidence-weighted cryoEM map optimisation.

Taking two half maps as input, LcoScale-2.0 produces feature-enhanced maps along with a robust confidence score that guides objective map interpretation.

cryotud.github.io/locscale/

Offre de thèse disponible dans notre équipe au @mmsb-lyon.bsky.social
Si vous êtes étudiant(e) en Master 2 et intéressé(e) par la génétique bactérienne, microscopie à épifluorescence et biochimie, contactez-nous et postulez à l'offre!
Dossier pour le 13/06
Concours école doctorale E2M2: 8 et 9/07.

Completely agree, these case studies are very useful and well made. Great material!

Due to the situation in the U.S. particularly for non-American scientists, we are exceptionally offering U.S.-based colleagues on line access to the #NMR conference.
If you’re registered, contact us to receive the link.
If not, contact us for registration and details👇nmr2025@conferences-pasteur.org