@NicoliLab

New preprint out!
In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...

🚨 New paper from our lab just published in Nature🚨

www.nature.com/articles/s41...

Discover how 🌱 tumour formation is shaped by tissue context from the very beginning. Cancer is not driven by genetics alone

✨Fantastic team led by Greta Skrupskelyte, Eduardo Rojo, @hariajith.bsky.social

Lymphatic disruption drives lung transplant fibrosis through interleukin-1–mediated hyaluronan accumulation Interleukin-1–mediated hyaluronan synthesis in transplanted grafts promotes fibrotic remodeling because of lymphatic disruption.

New work deciphers how disruptions to #lymphatic drainage drive fibrosis in transplanted lungs and hearts through the accumulation of hyaluronan—and identifies potential therapeutic targets to prevent lung fibrosis in mice. #ScienceTranslationalMedicine https://scim.ag/3ONyh7H

Keeping cells fit Fragments of aberrant cytoplasmic mRNA pair with nuclear RNAs to augment transcription

In a new Science study, researchers report on transcriptional adaptation, a dual feedback and feedforward mechanism that uses genetic redundancy to compensate for mutations in protein-coding genes.

Learn more in a new #SciencePerspective: https://scim.ag/4ryeXtN

Thanks of sharing ‪@phnglab.bsky.social, I almost missed it!!!
👏🏼👏🏼👏🏼

Historically Black US universities chase top research ranking Howard University is reaping the rewards of becoming the first such institution to reach ‘R1’ status.

Howard is the only historically Black college or university in the United States to have landed the coveted title of an R1 research university

go.nature.com/4tRldOI

Aww,🤩 thank you @haoyin.bsky.social for highlighting our work — truly appreciated!

Join us in Vienna for the biggest international 'FISH' meeting in 2026! No matter which fish is your favorite model - zebrafish, medaka, killifish, cavefish, Danionella, cichlids, stickleback, you name them...- this meeting is for you!

www.ezm2026.org

Abstract submission deadline is March 15th.

Shapeti, A., Barrasa-Fano, J., Abdel Fattah, A.R. et al. Force-mediated recruitment and reprogramming of healthy endothelial cells drive vascular lesion growth. Nat Commun 15, 8660 (2024). #EpithelialMechanics
buff.ly/lDPQ7rD

CXCR3 as a flow-sensitive modulator of developmental #Angiogenesis in #Zebrafish 🐟

CXCR3i (AMG487), CXCR3a (VUF11222), cxcr3 triple KO🐟▶️
⏫cranial vascularity
⏬pericyte investment (CXCR3▶️⏫pdgfb)

@astratman.bsky.social #ATVB 2024
www.ahajournals.org/doi/10.1161/...

Ribosomal RNA expansion segments mediate the oligomerization of inactive animal ribosomes Cells down-regulate protein synthesis when stressed to conserve energy and shift resources toward repair. We found that in some mammalian cells, including neurons, stress also resulted in the formatio...

Out now in @science.org
Ribosomal RNA expansion segments mediate the oligomerization of inactive animal ribosomes | Science www.science.org/doi/10.1126/...

RBPscan: A quantitative in vivo tool for profiling RNA-binding protein interactions Kretov et al. introduce RBPscan, an RNA-editing-based platform that measures how RNA-binding proteins engage their targets in living cells. This approach quantifies the binding strength of protein-RNA...

RBPscan: A quantitative in vivo tool for profiling RNA-binding protein interactions. Congrats to Dmitry A. Kretov &Daniel Cifuentes team! 👏🏼👏🏼👏🏼
www.cell.com/molecular-ce...

This’ll be a good one, for those interested!

Call for Abstracts
NEW-RNA Technologies in Biotech and Industry:
Innovations and Applications
www2.rnasociety.org/conferences/...

For those interested! Invited speakers include @nicolilab.bsky.social, @phnglab.bsky.social, @benjhogan.bsky.social, and Holger Gerhardt @mdc-berlin.bsky.social among others! 🐟🩸

Convergent evolution of scavenger cell development at brain borders - Nature Transcription factor osr2 is identified as a specific marker and regulator of mural lymphatic endothelial cell (muLEC) differentiation and maintenance, and muLECs and border-associated macrophages sha...

Congrats Ben Hogan lab!!
www.nature.com/articles/s41...

Really excited to share our new paper in @nature.com! We uncovered how a physical instability of the cytoplasm coupled with the cell cycle drives cytoplasmic partitioning in early embryos #zebrafish #drosophila. Read more in this🧵 www.nature.com/articles/s41... 🤩
@poldresden.bsky.social @mpi-cbg.de

Color photograph of Joan Steitz (Joan Argetsinger Steitz), the distinguished American molecular biologist and biochemist renowned for her groundbreaking discoveries in RNA biology, including the identification of small nuclear ribonucleoproteins (snRNPs) essential to RNA splicing. She is pictured in a close-up portrait within a laboratory or research setting, smiling warmly and directly at the camera with an engaging, approachable expression that conveys enthusiasm and expertise. Steitz has gray hair pulled back, striking blue eyes, and is wearing large, elaborate dangling earrings adorned with purple gemstones and metallic accents. She is dressed in a rich purple blouse. The softly blurred background includes scientific elements such as lab benches, equipment, monitors, charts, and partial signage, evoking the environment of her long career at Yale University where she served as Sterling Professor of Molecular Biophysics and Biochemistry. #JoanSteitz #MolecularBiology #WomenInScience #Biochemistry #RNA

Biochemist/molecular biologist Joan Steitz was born #OTD in 1941.

She (& team) figured out how our cells read/use genetic instructions to make proteins. A key person who helped crack the code on RNA—the molecule that acts like a messenger between DNA & and the proteins our bodies need. #WomenInSTEM

Schwayer, C., Shamipour, S., Pranjic-Ferscha, K., Schauer, A., Balda, M., Tada, M., Matter, K., & Heisenberg, C. P. (2019). Mechanosensation of Tight Junctions Depends on ZO-1 Phase Separation and Flow. Cell, 179(4), 937–952.e18. #EpithelialMechanics
buff.ly/8XyDQkj

Subcellular depletion of importin β1 impairs presynaptic local translation and spatial memory Axonal localization of importin β1 is required for presynaptic functions that support spatial memory tasks.

Scientists have mapped out an importin-based pathway that enables neurons to maintain synaptic plasticity and spatial memory despite their unusually elongated shape.

Learn more in #ScienceSignaling: https://scim.ag/4qIJWTi

Endogenous retroviruses synthesize heterologous chimeric RNAs to reinforce human early embryo development Zygotic genome activation (ZGA) failure leads to developmental arrest and poses a clinical challenge to women’s fertility. We observed that human embryos arresting at the eight-cell ZGA stage exhibite...

Out now in @science.org Endogenous retroviruses synthesize heterologous chimeric RNAs to reinforce human early embryo development | Science www.science.org/doi/10.1126/...

Metabolism: How pyruvate controls cell size

🔗 eLife Insight Articles delve into the real significance of the latest research. Read here: buff.ly/7o7IxtH.

DROSHA, DICER, and damage-induced long ncRNA control BMI1-dependent transcriptional repression at DNA double-strand break Genes near DNA double-strand breaks are silenced through a BMI1-dependent mechanism, while transcription of damage-induced lncRNAs (dilncRNAs), processed by DROSHA and DICER, occurs at break sites. Esposito and Capozzo et al. reconcile these opposing events, showing that the dilncRNA-DROSHA-DICER axis directs BMI1 recruitment, chromatin remodeling, and gene repression.

DROSHA, DICER, and damage-induced long ncRNA control BMI1-dependent transcriptional repression at DNA double-strand break

A 5-day protocol to generate hiPSC 3D Vascular Organoid😎
ETV2▶️Endothelial Cell
NKX3.1▶️Mural Cell

👉Perfusable, arteriovenous network in vivo
👉⏫Reperfusion in mouse diabetic limb ischemia
👉⏫transplanted islet performance

@meleromartinlab.bsky.social #CellStemCell 2025
www.cell.com/cell-stem-ce...

Reduced TBX5 dosage undermines developmental control of atrial cardiomyocyte identity in a model of human atrial disease - PubMed There is evidence for a shared genetic basis of atrial septal defects (ASDs) and atrial fibrillation (AF), but it remains unclear how genetic susceptibility leads to these distinct human atrial diseases. Here, we used directed differentiation of human induced pluripotent stem cell to ventricular or …

good new this morning: manuscript accepted! Final form of this pubmed.ncbi.nlm.nih.gov/40894762/ coming soon to @dev-journal.bsky.social. Congratulations to everyone from @ucsanfrancisco.bsky.social @ucsfanesthesia.bsky.social @gladstoneinst.bsky.social, and Alex Clark in @jsauce7.bsky.social's lab.

From earlier this week: Averting atrial fibrillation-induced neurocognitive and vascular dysfunction…

Elaine Y. Wan & team show reduction of mitochondrial ROS prevents vascular dysfunction, restores perfusion & improves cognitive flexibility in a mouse Afib model: insight.jci.org/articles/vie...

Actin cage for the synaptic vesicle liquid phase Brodin and Shupliakov demonstrate that synaptic activity triggers the formation of an actin cage that encapsulates the synaptic vesicle cluster. Disruption of this cage leads to the detachment of the cluster from the release site. These findings explain how the synaptic vesicle liquid phase can remain in place during activity.

Actin cage for the synaptic vesicle liquid phase

Metabolic environment-driven remodeling of mitochondrial ribosomes regulates translation and biogenesis

Metabolic environment-driven remodeling of mitochondrial ribosomes regulates translation and biogenesis
http://dlvr.it/TPh6Bf

Yifan Cheng, Chuankai Zhou & colleagues
@cp-molcell.bsky.social
#CellBio2025

How Do We Become the Gatekeepers? “Do you have a moment?” I turned and saw my senior colleague, Paul (not his real name), a mentor and sponsor, at my office door.“Come on in!” I said—excited to have a chat with him.We engaged in small...

Brilliant commentary by my colleague Valentina Greco @yaleschoolofmed.bsky.social
"real change starts when we face our own discomfort"

thefulcrum.us/inclusion-di...

Prenatal electroacupuncture modulates maternal-fetal immune activation via a brain-to-splenic signal Zhang et al. demonstrate that prenatal ES at ST36 acupoint protects against MIA-induced adverse pregnancy outcomes and abnormal behavior in offspring. The protective effects of ES depend on the PVN-vagus-α7nAChR-spleen neuroimmune circuit, highlighting a brain-spleen axis as a promising therapeutic target for pregnancy complications.

Prenatal electroacupuncture modulates maternal-fetal immune activation via a brain-to-splenic signal