Michalina Janiszewska

Structural insights into IMP2 dimerization and RNA binding IGF2BP2 (IMP2) is an RNA-binding protein that contributes to tumorigenesis and metabolic disorders. Structural studies focused on individual IMP2 doma…

IMP2 is an RNA-binding oncofetal protein we study in the context of brain tumors, but just published a collaborative paper hinting at its oligomerization! With help from Roy, Kojetin, Forli and Griffin labs - structural studies are needed to guide drug design

www.sciencedirect.com/science/arti...

Cell sorting based on single nucleotide variation enables characterization of mutation-dependent transcriptome and chromatin states Abstract. Point mutations in oncogenes and tumor suppressor genes are common drivers of tumorigenesis. Moreover, single nucleotide variants (SNVs) also con

Our new method is out in NAR! Need a way to separate cells with point mutation from WT? And run these cells through scRNA-seq or CUT&Tag? Try our STAR-FACS! We separated GBM TERT promoter mutant cells from WT here: academic.oup.com/nar/article/...

Data Scientist in Sutton | The Institute of Cancer Research View details and apply for this Data Scientist vacancy in Sutton. Salary : Salary range £39,805 to £49,023 (depending on the experience) Reporting to: Professor Trevor Graham ...

Calling data scientists: we have opening in our Data Science core @icr.ac.uk to lead work around cancer spatial biology and single cell analysis. These are staff scientist type positions, ideal for someone who wants to help drive computational research. Apply here: jobs.icr.ac.uk/vacancies/12...

Deciphering the longitudinal trajectories of glioblastoma ecosystems by integrative single-cell genomics - Nature Genetics Comparison of paired primary and recurrent glioblastomas at the single-cell transcriptomic level describes molecular and cellular trajectories associated with tumor recurrence, highlighting extensive ...

1/ Truly excited to share our new study that I had to privilege to co-lead during my PhD alongside great friends and collaborators @masashi-nomura.bsky.social, @kevin-johnson.bsky.social and Luciano Garofano, published at Nature Genetics @natureportfolio.nature.com!

www.nature.com/articles/s41...

The multilayered transcriptional architecture of glioblastoma ecosystems - Nature Genetics Integrated single-cell transcriptomic and genetic characterization of 121 adult glioblastomas identifies heterogeneity at cell type, cell state and baseline expression program levels associated with s...

1/ Thrilled to share our TWO back-to-back papers published in Nature Genetics today! www.nature.com/articles/s41... , www.nature.com/articles/s41...

Multiprobe Photoproximity Labeling of the EGFR Interactome in Glioblastoma Using Red-Light Photocatalytic proximity labeling has emerged as a valuable technique for studying interactions between biomolecules in a cellular context, providing precise spatiotemporal control over protein labeling. One significant advantage of these methods is their modularity, allowing the use of a single photocatalyst with different reactive probes to expand interactome coverage and capture diverse protein interactions. Despite these advances, fewer methods have been developed using red-light excitation, limiting the use of photoproximity labeling in more complex media such as tissues and animal models. Herein, we develop a platform for proximity labeling under red-light excitation, utilizing a single catalyst and two distinct probe types. We first design a carbene based labeling system that utilizes sulfonium diazo probes. This system is successfully applied on A549 cells to capture the interactome of epidermal growth factor receptor (EGFR) using a Cetuximab-Chlorin e6 conjugate. Benchmarking against established techniques indicates that this approach performs comparably to leading carbene-based proximity labeling methods. Next, we leverage the strong singlet oxygen generation (SOG) ability of Chlorin e6 to establish an alternative labeling system using aniline and hydrazide probes. EGFR directed chemoproteomics experiments reveal significant overlap with the carbene system, with the carbene approach capturing a subset of interactions identified by the SOG system. Finally, we deploy our approach for the characterization of EGFR in resected human glioblastoma (GBM) tissue samples removed from distinct locations in the same tumor, representing the tumor’s infiltrating edge and its viable center, identifying several GBM specific interacting proteins that may serve as a launch point for future therapeutic campaigns.

Our first paper is out today in @jamchemsoc.bsky.social!
This is the beginning of a long journey for us, to study heterogeneity in GBM patient samples.
First step was to figure out some chemistry to enable us to look at this challenging problem.

pubs.acs.org/doi/10.1021/...

Our #Cancer #Evolution summer school runs again 30 June-3 July at the beautiful Genome Campus in Hinxton, UK sponsored by @wellcometrust.bsky.social

Ideal for starters in the field; this year's focus is on using genomic data for cancer evo research.

Brilliant & inspiring faculty! bit.ly/40nLdDD

EPFL Life Sciences Early Independent Research Scholar (ELISIR) From exceptional PhD directly to independent group leader

Fantastic opportunity: 🚀 Calling early-career scientists! Apply for the 2025 ELISIR Scholar at EPFL. From outstanding PhD straight to group leader. Join an interdisciplinary research community to conduct independent research in any area of #lifesciences in Lausanne, CH
👉 go.epfl.ch/ELISIR