Znger4

A super detailed protocol + video on Cryo-ExM - Cryo-Expansion Microscopy, led by the labs of our former postdocs @marinelap.bsky.social & @ebertiaux.bsky.social.
Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...

‘Borrowed time’: crop pests and food losses supercharged by climate crisis Heating means pests breeding and spreading faster, warn scientists, with simplified current food system already vulnerable

The key global crops, wheat, rice and maize, are expected to see the losses to pests increase by about 46%, 19% and 31% respectively when global heating reaches 2C 👇 www.theguardian.com/global-devel...

New EMBO J

𝘔𝘺𝘤𝘰𝘣𝘢𝘤𝘵𝘦𝘳𝘪𝘶𝘮 𝘵𝘶𝘣𝘦𝘳𝘤𝘶𝘭𝘰𝘴𝘪𝘴 uses “on-off switch” to pause or restart its growth

DarT tags DNA to halt replication, DarG removes the tag to resume activity

Stop-start mechanism helps TB evade antibiotics and immune clearance, establish chronic infection

www.embopress.org/doi/full/10....

Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase Programmable gene integration in human cells has the potential to enable mutation-agnostic treatments for loss-of-function genetic diseases and facilitate many applications in the life sciences. CRISP...

Excited to introduce evoCAST: a platform for efficient, programmable gene integration in human cells. This work, a close collab between my PhD lab (Liu group) and @sternberglab.bsky.social, has been such a fun journey to help lead. Huge thanks to all contributors!

www.science.org/doi/10.1126/...

Powerful CRISPR system inserts whole gene into human DNA ‘Directed’ evolution in the laboratory creates an editing tool that outperforms classic CRISPR systems.

www.nature.com/articles/d41...
Coverage of our collaboration with the Liu group..
' “This is crazy directed evolution!” - Makoto Saito'

Nearly 100% of bacterial infections can now be identified in under 3 hours Scientists have made a major breakthrough in the accuracy and speed at which often deadly pathogen infections can be identified and treated. This is time that, in many cases, is critical to saving a p...

newatlas.com/imaging-diag...

Tomorrow, Tuesday March 25th at 11am ET, @jpassmore.bsky.social from Utrecht University, The Netherlands, will present "Outcome-driven microscopy: integrating smart microscopy and optogenetics for precise biological control"!

Join us on Zoom, more information on biocontrolseminars.org

Chart showing the chemical structures of the common amino acids as well as brief notes of their tastes. The latter are taken from: https://doi.org/10.3758/BF03203878 https://doi.org/10.1016/0031-9384(81)90298-5 The original chart is modified from one made by @etymology_nerd on "How amino acids got their names" available e.g. here: https://x.com/etymology_nerd/status/1310560462329831425

How amino acids taste 🧪

A number of people who run those resources were contractors. In December, some of them were transitioned into Federal employee status to protect them.

Unfortunately, this means they were probably on the list of employees with probationary status.

I am trying to find out what actually happened.

Birth defects are the #1 cause of death in the first year of life (🧵). Birth defects kill more than twice as many children as cancer. Birth defects are diseases of developmental biology. Here are CDC statistics for 2021. 1/5

wisqars.cdc.gov/pdfs/leading...

Cancer cells ‘poison’ the immune system with tainted mitochondria Immune cells lose their cancer-fighting prowess after taking tumours’ organelles on board.

Really interesting paper 🧪 🤩

Immune evasion through mitochondrial transfer 😮

Ikeda et al find that #mitochondria from cancer cells can #transfer over to T cells - altering their #metabolism & reducing antitumour immunity

www.nature.com/articles/s41...

www.nature.com/articles/d41...

@nature.com

Multiplex genome editing eliminates lactate production without impacting growth rate in mammalian cells Nature Metabolism - Hefzi et al. engineer cells to nearly eliminate lactate production and increase mitochondrial use of pyruvate by deleting both lactate dehydrogenases and pyruvate dehydrogenase...

Here's a link to the pdf: rdcu.be/d6aZw

A brief history of protein structure prediction and design YouTube video by RosettaCommons

A seminar on the history of protein structure prediction and protein design. By Mohammed AlQuraishi
youtu.be/Ahwn-KS8fd0?...

To me, it looks like a Reduviids, predatory insect.

MSK Kids: First in New York City Offering Gene Therapy for Sickle Cell Disease and Beta Thalassemia A Q&A with Jaap-Jan Boelens, MD, PhD, Chief of the Pediatric Transplant and Cellular Therapy Service at MSK.

1/2: MSK is the first hospital in New York City to offer gene therapy for sickle cell disease and beta thalassemia through its pediatric program, MSK Kids. Learn more: bit.ly/4gUKC3a

Systems-level immunomonitoring in children with solid tumors to enable precision medicine In a population-based cohort of 191 children with diverse solid tumors, systems-level analyses unravel immune variation with age and tumor type and provide a reference for future precision immunothera...

191 children with diverse solid tumors, immune variation with age and tumor type provide a reference for future precision immunotherapies tailored for the evolving immune systems of children
www.cell.com/cell/fulltex...
@cellcellpress.bsky.social @petterbrodin.bsky.social

Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19 SARS-CoV-2 infection can lead to long-lasting neurological sequelae, but underlying mechanisms are unclear. Rong et al. report that SARS-CoV-2 spike protein persists in the skull-meninges-brain axis, ...

New paper from our lab @erturklab.bsky.social

www.cell.com/cell-host-mi...