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Milstein Lab @ UofT

@milsteinlab

Biological Physics at #UofT. We study (primarily) bacteria using single-molecule and single-cell techniques. http://www.utm.utoronto.ca/milsteinlab

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29.10.2024
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Latest posts by Milstein Lab @ UofT @milsteinlab

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The Biophysical Society of Canada is pleased to announce that Dr. Sarah Rauscher @sarahrauscher.bsky.social is the recipient of the 2026 Early Career Investigator Award. Congratulations!!

01.03.2026 00:59 πŸ‘ 8 πŸ” 3 πŸ’¬ 1 πŸ“Œ 1
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INSPIRE | Office of the Vice-Principal Academic and Dean U of T's Interdisciplinary Science Program in Research & Entrepreneurship Discover the Quantitative BiosciencesJune 15–26, 2026Join a two‑week immersive research experience where you’ll explore unanswered biological questions inside state‑of‑the‑art laboratories.

#INSPIRE2026 is now open for registration. Spend 2-weeks getting hands on experience in #QuantitativeBiology and #ResearchTranslation @utm.utoronto.ca

www.utm.utoronto.ca/dean/inspire

27.02.2026 14:49 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Just out…our recent preprint on how she shapes of #bacteria can affect the stability of the #microbial communities they form

www.biorxiv.org/content/10.6...

27.02.2026 14:45 πŸ‘ 5 πŸ” 1 πŸ’¬ 1 πŸ“Œ 0
2025 INSPIRE Scholars Program
2025 INSPIRE Scholars Program YouTube video by Office of VP Research & Innovation at UTM

Learn more about #INSPIRE, a program we developed at the interface of #biophysics, drug development, #innovation and #entrepreneurship

www.youtube.com/watch?v=EmwL...

30.07.2025 21:17 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Enhancing the applied force and range of axial optical tweezers Axial optical tweezers provide a natural geometry for performing biomechanical assays, such as rupture force measurements of protein binding. Axial traps, however, are typically weaker than their late...

At long last, are newest article on "Enhancing the applied force and range of axial optical tweezers” is out. #singlemolecule #biophysics

www.cell.com/biophysrepor...

29.06.2025 13:07 πŸ‘ 5 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Our experimental lab w/ the Hilfinger lab @uoft.bsky.social are jointly looking to hire a #postdoc in experimental biological #physics. Exploring noise and dynamics within single-cells and bacterial populations. Please share with anyone you think might be interested.

17.06.2025 21:34 πŸ‘ 6 πŸ” 4 πŸ’¬ 1 πŸ“Œ 0

Here’s a great opportunity if you know of anyone looking for a postdoc.

02.06.2025 16:43 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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An expectation–maximization approach to quantifying protein stoichiometry with single-molecule imaging AbstractMotivation. Single-molecule localization microscopy (SMLM) is a super-resolution technique capable of rendering nanometer scale images of cellular

This is based on our publication in #BioinformaticsAdvances, but is much much faster, does all the preprocessing for you, and has a nice GUI.

academic.oup.com/bioinformati...

15.04.2025 19:14 πŸ‘ 3 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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GitHub - thericshi/protein-stoichiometry-quantifier Contribute to thericshi/protein-stoichiometry-quantifier development by creating an account on GitHub.

If anyone is interested, we’ve developed a nice GUI in Python for molecular counting with #SMLM. Just give it a localization file and it will estimate the monomer/dimer fraction.

github.com/thericshi/pr...

15.04.2025 19:11 πŸ‘ 4 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

A big shout out to everyone who’s marching at today’s #standupforscience events.

07.03.2025 12:27 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Nitroreductase-activatable photosensitizers for selective antimicrobial photodynamic therapy Antimicrobial photodynamic therapy (aPDT) utilizes light, oxygen and a photosensitizer (PS) to enact cell death via the production of reactive oxygen species (ROS). This mechanism of cell death, via o...

Another successful collaboration with the Beharry lab on #antimicrobial photodynamic therapy. This just out in @rscmedchem.rsc.org:
pubs.rsc.org/en/content/a...

06.03.2025 12:41 πŸ‘ 3 πŸ” 2 πŸ’¬ 0 πŸ“Œ 0
Question: How much effort should go into library work?

Hamming: It depends upon the field. I will say this about it. There was a fellow at Bell Labs, a very, very, smart guy. He was always in the library; he read everything. If you wanted references, you went to him and he gave you all kinds of references. But in the middle of forming these theories, I formed a proposition: there would be no effect named after him in the long run. He is now retired from Bell Labs and is an Adjunct Professor. He was very valuable; I'm not questioning that. He wrote some very good Physical Review articles; but there's no effect named after him because he read too much. If you read all the time what other people have done you will think the way they thought. If you want to think new thoughts that are different, then do what a lot of creative people do - get the problem reasonably clear and then refuse to look at any answers until you've thought the problem through carefully how you would do it, how you could slightly change the problem to be the correct one. So yes, you need to keep up. You need to keep up more to find out what the problems are than to read to find the solutions. The reading is necessary to know what is going on and what is possible. But reading to get the solutions does not seem to be the way to do great research. So I'll give you two answers. You read; but it is not the amount, it is the way you read that counts.

Question: How much effort should go into library work? Hamming: It depends upon the field. I will say this about it. There was a fellow at Bell Labs, a very, very, smart guy. He was always in the library; he read everything. If you wanted references, you went to him and he gave you all kinds of references. But in the middle of forming these theories, I formed a proposition: there would be no effect named after him in the long run. He is now retired from Bell Labs and is an Adjunct Professor. He was very valuable; I'm not questioning that. He wrote some very good Physical Review articles; but there's no effect named after him because he read too much. If you read all the time what other people have done you will think the way they thought. If you want to think new thoughts that are different, then do what a lot of creative people do - get the problem reasonably clear and then refuse to look at any answers until you've thought the problem through carefully how you would do it, how you could slightly change the problem to be the correct one. So yes, you need to keep up. You need to keep up more to find out what the problems are than to read to find the solutions. The reading is necessary to know what is going on and what is possible. But reading to get the solutions does not seem to be the way to do great research. So I'll give you two answers. You read; but it is not the amount, it is the way you read that counts.

Sometimes reading Hamming makes me sad, because I recognize myself in this quote.

25.02.2025 09:17 πŸ‘ 27 πŸ” 4 πŸ’¬ 3 πŸ“Œ 2
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Here’s an event I would highly recommend. Just around the corner if you’re in the GTA.

19.02.2025 14:31 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Dear Future Premier, science needs to be a priority. I just signed a letter to put science and evidence-informed decision-making at the heart of our provincial elections!πŸ—³οΈπŸ”¬ In the upcoming elections in Ontario, it's crucial that our future premiers pr...

actionnetwork.org/petitions/de...

06.02.2025 18:52 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Lots of #imaging and #microscopy workshops coming up in Canada. Just thought I would share. Thanks @bioimagingna.bsky.social and #CanadaBioimaging

06.02.2025 15:49 πŸ‘ 3 πŸ” 3 πŸ’¬ 0 πŸ“Œ 0
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Here’s a bit more information on #INSPIRE2024. Feel free to print out and post.

21.01.2025 18:48 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Registration for #INSPIRE2025 is open to all #Physics, #Chemistry, and #Biology undergraduates with an interest in Quantitative Biological and Translational Sciences. Work directly in labs @uoft.bsky.social @uoftmississauga.bsky.social while also learning about #innovation and #entrepreneurship.

10.01.2025 15:09 πŸ‘ 4 πŸ” 1 πŸ’¬ 0 πŸ“Œ 1
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Look what I just found in my mailbox. It’s nice to finally see this is print.

07.01.2025 18:47 πŸ‘ 9 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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Why Canada excels at medical tech startups The Globe and Mail offers the most authoritative news in Canada, featuring national and international news

Pleased to see that #SpinUP made the news. Lot’s of innovative research translation efforts happening here @uoftmississauga.bsky.social @uoft.bsky.social

www.theglobeandmail.com/business/art...

10.12.2024 16:46 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

Elegoo Saturn 2 8K Resin 3D Printer. That’s about $400 CAD!

10.12.2024 15:59 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

We simply used an Elegoo Saturn 2. About $400 CAD!

10.12.2024 15:58 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
Serpentine mixer mold

Serpentine mixer mold

Forget a clean room, we can do most of our larger feature #microfluidics on a resin based #3Dprinter. Here’s a PDMS mold for a serpentine mixer that you can even bake at 120C.

09.12.2024 18:18 πŸ‘ 13 πŸ” 2 πŸ’¬ 1 πŸ“Œ 1
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CRAFT - Centre for Research and Application in Fluidic Technologies Latest news at CRAFT Read all stories Publications Invention Disclosures Patent Filings Developing new and innovative microfluidic devices Multidisciplinary teams creating new technolo...

We mostly do our own photolithography at a core facility on campus (craftmicrofluidics.ca). But for the Mother Machine video I posted, we actually inherited those molds from a colleagues lab.

04.12.2024 19:08 πŸ‘ 1 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0
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Finally starting to get this system working. While these movies might not look too impressive, they make me feel good. #microfluidics #biophysics

04.12.2024 14:51 πŸ‘ 17 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0

Would be happy to participate if you need any speakers for next year.

04.12.2024 14:34 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

Please add..

04.12.2024 02:05 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
Assistant Professor - Theoretical Biological Physics Assistant Professor - Theoretical Biological Physics

We’re looking to hire a theoretical #biological #physicist at either the Assistant or Associate professor level. The post closes soon, so please spread the word. #Toronto is a great place to live and work.

jobs.utoronto.ca/job/Toronto-...

jobs.utoronto.ca/job/Toronto-...

21.11.2024 23:53 πŸ‘ 7 πŸ” 11 πŸ’¬ 0 πŸ“Œ 0
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Congrats to Zhang and Tianyi on successfully pulling off their #PhDs! Now I need more students…so if you know of anyone...

18.11.2024 15:19 πŸ‘ 3 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

Could you add us please?

17.11.2024 18:55 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

Please add my lab as well. Cheers.

17.11.2024 14:17 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0