Hauke Drechsler

Commercial CO2-cylinder of a water carbonator connected to a scientific technical device that produces a mixed atmosphere for cells during live cell microscopy.

What's good for us can't be bad for our cell lines... eh?!🤷🏼‍♂️

To enable reuse by the research community we're making the two parental FPBase libraries available on @addgene.bsky.social as pooled plasmid libraries (Addgene #245482 #245483). Use them. Shuffle them. Train on them! addgene.org/Calin_Plesa/ 12/n

We are very excited to share a new resource from our team: spatial subcellular proteome maps in plants! We developed an MS-based method that registers localizations of about 8000 proteins in Arabidopsis roots in a single experiment.

(1/9)
www.biorxiv.org/cgi/content/...

ATTENTION #tubulin fans! We have another #openaccess #Techniques and Procedures paper in #CYTOSKELETON! Expression, Purification, and #Microscopy-Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 #Human Tubulin. Read it here: onlinelibrary.wiley.com/doi/10.1002/...

Microscopy images showing the heterogeneity of cultured astrocytes.

Chieko Ikoma, Kodai Inoue, Asako Terasaki and colleagues use their improved culture and transfection methods to visualise cytoskeleton structures in primary cultured astrocytes.
Highlight: journals.biologists.com/jcs/article/...
Article: journals.biologists.com/jcs/article/...

ATTENTION #KINESIN FANS!!! We have an #openaccess Techniques and Procedures paper for you in #CYTOSKELETON! Protocol for Reconstituting Adaptor-Mediated Activation of Full-Length Kinesin-1. Read it here: onlinelibrary.wiley.com/doi/10.1002/...

The peer reviewed and updated version of this work is now online (although not quite the fully formatted version). We had an existential crisis about kinesin-1 regulation while developing this work, but we are super proud of everything we put into this project. www.cell.com/iscience/ful...

image of the OpenFlexure Microscope on the left. On the right, our adapted live-cell imaging openflexure microscope with cradle to negate vibrations inside an existing incubator

Unleashing live-cell imaging for the masses!
Adapting the OpenFlexure Microscope for Affordable Live-Cell Imaging www.biorxiv.org/content/10.6...
Fits into existing incubators for time-lapse experiments & feeds into existing bioimaging analysis pipelines.
Thanks @wellcometrust.bsky.social funding

ATTENTION #TUBULIN FANS!!! Have a look at this outstanding #openaccess Perspective in #CYTOSKELETON! C-Terminal Tail Elongation Adds a New Dimension to the Tubulin Code. onlinelibrary.wiley.com/doi/full/10....

📣 Please spread: we’re hiring a Lab Manager! It is a critical position in our new lab. As our lab moves to Barcelona and joins the @crg.eu, we’re looking for a Lab Manager to help build the lab and set the lab atmosphere.
🔗 Apply here 👉 recruitment.crg.eu/content/jobs...

🔬🚨New preprint alert! 🚨🔬

We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology

www.biorxiv.org/content/10.6...

#SuperResolution #CellBiology

How to discovery new regulators of cell migration using a high-throughout assay?

Make use of our new label-free deep learning-based automated tracking assay DeepBIT.

To appear in Science Advances.

Read about DeepBIT here: www.biorxiv.org/content/10.1...

Our paper describing the development of ShapeSpaceExplorer is now out: journals.plos.org/ploscompbiol... The software and documentation is available here: github.com/cmcb-warwick... Explainer thread below.

Not that this passes without being noticed: The GreenRobust cluster of excellence is officially alive since Jan 1, 2026! Looking forward to at least seven years of exciting science in the field of plant ecology, molecular biology, agriculture and the combination of all these fields. Stay tuned!

Fig. 1 (shortened, full legend in paper): Challenges associated with studying microtubules, along with novel technologies that can overcome these obstacles. (A) Microtubule organization within a plant cell is complex and ever evolving. A wealth of microtubule-associated proteins (MAPs) are expressed in a cell type-specific and cell cycle-dependent manner to control microtubule kinetics. Together with the intrinsic instability of microtubules, MAP expression leads to highly dynamic microtubule arrays that are locally regulated and undergo rapid transitions, making it imperative to develop ways to capture them with high spatiotemporal resolution. (B) Super-resolution microscopy enables nanometer-scale visualization of microtubule networks, overcoming the resolution limits of conventional microscopy.

🗂️🔬 SPECIAL ISSUE REVIEW 🗂️🔬

In this review, Park & Muroyama discuss the technological advances that are generating new insights into the organization and functions of microtubules in plant cells.

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪

Now that you have gathered you raw data from a cell migration assay, how do you analyze that data?

In our review, we describe methods - including AI methods - to track migrating cells and extract as much information as possible.

Read our paper here: www.nature.com/articles/s41...

MT staining was always nice, kinesin a bit more difficult.

MetOH fixation, Primary Antibody, Crosslinking with PFA/AA, TREx gelation, Heat/SDS denaturing, Primary Antibody again, Secondary Antibody, Expansion, mounting on plasma cleaned APTES treated coverslips.

Roughly 7.3x

Fluorescence micrograph of a metaphase cell showing (spindle) microtubules in green, chromosomes in blue and some pink staining on the spindle poles.

Behold the beauty of a human metaphase spindle 🫠

[RPE1 cell, confocal expansion microscopy, microtubules in green, chromosomes in blue, kinesin-12 in magenta)

micrograph by Malte Lemmin, Nanoscience Master Student @zmbp-tuebingen.bsky.social @unituebingen.bsky.social

New release of #BigVolumeBrowser. Many things were added: UI improvement (thanks to @kwolbachia.bsky.social feedback), pyramidization for BioFormats dataset, bug fixes.
Now there is an isosurface rendering mode (1/4)

PDB101: Learn: Flyers, Posters, & Calendars: Posters: Molecular Machinery: A Tour of the Protein Data Bank The enormous range of molecular shapes and sizes in the PDB archive is illustrated using 96 structures, depicted relative to the cellular membrane.

Take a tour of the PDB--The vast range of molecular shapes and sizes in the archive are illustrated by these 96 biomolecular machines.

Fact!

Just look at those microtubules go! Happy to see our paper out in JCS ✨

View across a valley filled with fog. Some trees are visible as vague schemes. The sun shines through some dispersed clouds in an otherwise blue sky.

Working above the clouds today ...

Ein Wachsmalstift steht neben einem kleinen Wachsmalstift Stummel, der auf dem Boden sitzt. Der große sagt zum kleinen Stift: Wachs mal, Stift!

Witztürchen Nr. 7

#witztürchen

New to RNA design? Please try pyFuRNAce! Looking forward to your feedback 🧬

Have to feel for the grey eduWOSM. It's just so .. grey ..

How time flies...

Mechanochemistry of Molecular Motors and Filaments. 8-9 May 2026 at University of Warwick.

Calling all #MotorsInQuarantine fans: Please join us for 1.5 days of science on motors and the cytoskeleton as we celebrate the career of Rob Cross. More info and sign-up here: tinyurl.com/mechanochemi... #kinesin #dynein #myosin #microtubules #actin #cytoskeleton