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01.03.2026 15:54
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Mast cell extracellular granules are found to be condensates formed through sugar-metabolite interactions with elevated pH and higher metal concentration that enrich and enhance the activity of immune modulators, such as cytokines and proteases
www.nature.com/articles/s41...
Congratulations to new Sloan Fellow @grant.rotskoff.cc @stanfordchemistry.bsky.social !!
sloan.org/fellowships/...
Happy Chinese New YearοΌπ΄
A horse year 2026οΌ
Nice coverοΌHappy Valentineβs Dayπ
Excellent workοΌ
How did life arise from simple chemical building blocks?
New #LMBResearch led by @edogia.bsky.social in @philholliger.bsky.social group has identified a small self-replicating ribozyme that could be the answer.
Read more: mrclmb.ac.uk/news-events/...
Thank you for highlighting our work with very visionary perspectives! @mstagi.bsky.social @natcellbio.nature.com
Decoding the proton veil around lysosomes
www.nature.com/articles/s41...
π
π’ #RSCChemBio is sponsoring two poster prizes at the EMBO-EMBL Chemical Biology Workshop 2026!
π
8 - 11th September 2026
π EMBL Heidelberg, Germany
π www.embl.org/about/i...
Submit your abstract by 2nd June 2026 to a part of the event!
EMBO #ChemicalBiology
Thanks!!ππβΊοΈ Great tools!
Most importantly, the key is to figure out the downstream biosensor. By lyso-IP experiments that is to isolate lysosome in acidic buffer and neutral buffer, we found that RILP is recruited more under acidic pH buffer! We also figure out the potential site, by a seires of biochemistry experiments.
Lysosome mobility is considered to be luminal-pH related. However, we all know that, dynein and adaptor and proteins are assembled on the surface, therefore I guess, it is juxta-lysosome pH regulates lysosome pH. By experiments, we verified, yes, it is.
We also change the location of proton-sensitive dye on this DNA duplex, to change its distance from the lysosomal surface. We measure that how long it will take to reach the same pH with cytosol?
To our surprise, this acidic juxta-lysosome pH has a thickness!!
We then explored that, TMEM175, a proton-efflux channel, maintained this aicidic juxta-lysosome pH.
Knocking out TM175 increase it and verse vise. Stimulating TM175 will decrease juxta-lysosome pH, which is negligable in TM175-KO cells.
Then, by conjugating proton-based ratiometric small molecules on the DNA duplex, we measured that, the surface pH of lysosomes is lower than neutral cytosol! We named it juxta-lysosome pH. It is 0.2-0.7 pH unit lower than cytosol.
First step, we establish a platform technology via DNA nanotechnology on the lysosome surface.
@carolynbertozzi.bskyverified.social acidic nanolayer is finally out! thank you for enlightening me in Shanghai last summer!
Beyond thrilled to share my PhD work out today in @natcellbio.nature.com , uncovering a nanoscale acidic environment around lysosomes that controls their mobility through proton signaling.π₯³π₯³π₯³
Great thanks to my advisors and excellent peers for helping meοΌ
www.nature.com/articles/s41...
Congrats to my advisor Weihong and teamοΌ
doi.org/10.1126/scie...
CheersοΌFinally get my doctoral diploma and become Dr.Zhangπ₯³π₯³ Look forwardοΌ
That is interestingοΌProton is magic-The most fundamental but important ion. I really like itπ€©π€©π€©
I have another question that I'm curious very much. What do you think of the biology function of these pH gradient inside organelles compared to the uniform distributed pH we considered in the pastοΌ
excellent depict of organelle pH! Proton signaling is promising.
ICYMI: Molecular bioengineering of functional nucleic acids
First time to make an offline flash talk to present my PhD workπ€©π€ Really good opportunityπ
Glad to share my work and to receive first prize award of poster
οΌπ€©π€©π€©
These intriguing materials - the world's most porous sponges - won today's Nobel Prize in Chemistry
go.nature.com/3VVjXug
