@veselin-nasufovic
Associate Principal Scientist, Organic Chemist in Bayer AG. Postdoc in Johnsson lab, MPI for Medical Research, Heidelberg. PhD from FSU Jena, Arndt group. Opinions are mine, I do not speak in name of my employer in any way.
Fast and Luminous: Clip-tag2. As fast as Halo and SNAP-tag2 with some benefits of redesigned substrate, PF instead of BC is from now on CLIP team. We look forward seeing cool applications in multiplexing, CID, sensors, and maybe some new surprising ones!
A Cinderella story: raising CLIP-tag from the ashes to shine in fluorescence labeling.
www.biorxiv.org/content/10.6...
Looking forward Stephan of course! Again, amazing work, great for the community and your team
Great work, and congratulations Stephan and the whole team. I can not skip asking about submission date in 2021. What happened in the meantime that they kept it on and off so long?
Happy to hear Robert, please share with the community whenever you have insights about properties (good or bad of course)
Amazing study! Happy to see XActin (FastActX) probes in use
Here is a small pre-print from Arndt Group @FSUJena about SAR which led to the development of SiR-XActin. Thanks to everyone involved! chemrxiv.org/engage/chemr...
Happy to see what XActin probes can do (Spy555-FastAct_X) <3
📣Paper alert! Interested in faster and brighter protein labeling than before? 👀 @steffi-k.bsky.social & other colleagues have developed an improved tool for labeling proteins with synthetic fluorophores for bioimaging. Also works in STED microscopy 🤩🔬 Congratulations!🥳
www.nature.com/articles/s41...
Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging:
www.biorxiv.org/content/10.1...
Congratulations to Julian Kompa and the entire team.
SiR-XActin, SPY650-Fastact and SiR-actin, along with whole jasplakinolide-like actin ligand based tools are cell permeable small molecules which do not require genetic manipulation. Dissolve in DMSO, add some to the medium and image with your favourite microscope :)
Check out our new fluorescent probe for imaging f-actin dynamics: 𝗦𝗶𝗥-𝗫𝗔𝗰𝘁𝗶𝗻: 𝗔 𝗳𝗹𝘂𝗼𝗿𝗲𝘀𝗰𝗲𝗻𝘁 𝗽𝗿𝗼𝗯𝗲 𝗳𝗼𝗿 𝗶𝗺𝗮𝗴𝗶𝗻𝗴 𝗮𝗰𝘁𝗶𝗻 𝗱𝘆𝗻𝗮𝗺𝗶𝗰𝘀 𝗶𝗻 𝗹𝗶𝘃𝗲 𝗰𝗲𝗹𝗹𝘀 www.biorxiv.org/content/10.1...
Thank you @veselin-nasufovic.bsky.social
Can you spot the difference? 🔬
SiR-actin labeling can be cytotoxic and affects actin dynamics - while the new XActin probes do not! Developed by @veselin-nasufovic.bsky.social and perfect for time-resolved and STED imaging without the need for genetic manipulation.
www.biorxiv.org/content/10.1...
New actin probe from @veselin-nasufovic.bsky.social et al! SiR-XActin and its variants are more photostable and less perturbative than SiR-actin or FastAct. And it's already available from @spirochrome.com! With some beautiful data from @chillinwithpfn1.bsky.social:
www.biorxiv.org/content/10.1...
🙏A huge thanks to all coauthors for making this possible: @kjohnsson.bsky.social , Hans-Dieter Arndt, @juliankompa.bsky.social, Ada Cavalcanti, @chillinwithpfn1.bsky.social , Halli L. Lindamood, Merle Blümke, Birgit Koch, Victoria Levario Diaz, Katharina Weber, Marlene Maager.
We hope that #SiR-XActin will continue the legacy of older relative #SiR-actin and become an indispensable tool in live-cell no-wash imaging, cell painting assays and cell biology in general.
4/5 🔬 We have demonstrated that #𝗫𝗔𝗰𝘁𝗶𝗻 spectral properties can be tuned with other #SiR and #MAP fluorophores covering the important part of the imaging window.
3/5 💡 The key to our success was a careful #cryo-EM structure-driven SAR study of the #XActin ligand, in which we synthesized and tested more than 100 cycic peptides. We minimized cytotoxicity and preserved its binding to F-actin.
2/5 🌟 We've developed a new fluorescent probe for live-cell no-wash imaging with enhanced photostability and no observable cytotoxicity. 𝗦𝗶𝗥-𝗫𝗔𝗰𝘁𝗶𝗻 enables imaging of actin dynamics without induction of non-physiological phenotypes.
1/5 🚀 Excited to share our latest preprint from: 𝗦𝗶𝗥-𝗫𝗔𝗰𝘁𝗶𝗻: 𝗔 𝗳𝗹𝘂𝗼𝗿𝗲𝘀𝗰𝗲𝗻𝘁 𝗽𝗿𝗼𝗯𝗲 𝗳𝗼𝗿 𝗶𝗺𝗮𝗴𝗶𝗻𝗴 𝗮𝗰𝘁𝗶𝗻 𝗱𝘆𝗻𝗮𝗺𝗶𝗰𝘀 𝗶𝗻 𝗹𝗶𝘃𝗲 𝗰𝗲𝗹𝗹𝘀 on #bioRxiv!
www.biorxiv.org/content/10.1...
#JohnssonLab and #ArndtLab with valued collaborators
Thrilled to share with you our work on covalent probes! By introducing a biocompatible sulfonium-based cleavable linker, we developed a series of fluorescent probes that can label covalently endogenous tubulin in various cell lines.
www.biorxiv.org/content/10.1...
#Chembio #FluorescenceFriday
I would like to draw your attention to this job: Scientist Proteomics/Chemical Biology talent.bayer.com/careers/job/...
Chemical Biology, Imaging and Omics group is really great please to work. We will be very happy to get new motivated colleague.
Cool paper from Cravatt lab: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes: www.cell.com/cell-chemica...
The "Fluorescent Probes, Sensors and Fluorophores" starter pack got substantial additions worth a new look.
Please raise you hand 👋 in the replies if you want to be added too.
go.bsky.app/MtgJE4w