Sebastian Glatt

RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity - Nature Cas12a3 nucleases constitute a distinct clade of type V CRISPR–Cas bacterial immune systems that preferentially cleave the 3′ tails of tRNAs after recognition of target RNA to induce growth arres...

Our paper is out! Hiding in plain sight among Cas12a nucleases, Cas12a3 cleaves not its RNA target but the 3′ ends of tRNA. Huge thanks to all who made this possible, especially the Beisel lab, Biao & Dirk for the structure, & @sebastianglatt.bsky.social for all things tRNA. doi.org/10.1038/s415...

Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...

We're hosting the 8th Austrian CryoEM symposium at ISTA Nov 11-12 @istaresearch.bsky.social this year - you don't want to miss! We have a fantastic lineup of invited speakers + selected talks from abstracts #cryoEM
Deadline for abstracts Oct 15
Final deadline Oct 31
cryoem-symposium.pages.ist.ac.at

In this article, we highlight recent advances in the field, showcase the outstanding work from many labs worldwide, and provide a unique overview of currently available structural data on RNA modifications across different ribosomes.

Mapping rRNA, tRNA, and mRNA modifications in ribosomes at high resolution Chemical modifications of rRNA, tRNA, and mRNA play key roles in protein synthesis by affecting the structure of these RNAs, by modulating decoding, a…

I'm excited to share that our latest review, “Mapping rRNA, tRNA, and mRNA Modifications in Ribosomes at High Resolution”, has just been published in @cp-trendsbiochem.bsky.social!
Read the full review here 👉 www.sciencedirect.com/science/arti...

Mechanistic insight into the pseudouridylation of RNA Pseudouridylation (Ψ) is a highly abundant and conserved RNA modification that is present in all three domains of life. The incorporation of Ψ can affect the stability of RNAs, modulate their inter...

Happy to share our latest review on PUS enzymes, which just got published in RNA Biology. Please have a look, if you want to know more about the enzymes that introduce Pseudouridine in RNA molecules.

www.tandfonline.com/doi/full/10....

Congrats to two fantastic PhD students, Wei Guo and
@igorkaczmarczyk.bsky.social, who figured out that Q is indeed incorporated in certain precursors-tRNA and how the modifying complex (QTRT1/2) manages to do so...

Queuosine is incorporated into precursor tRNA before splicing - Nature Communications tRNA modifications are essential for function, yet their timing relative to processing is unclear. Here, the authors show that queuosine and derivative modifications occur before splicing of pre-tRNA^...

We are excited to share our latest research on Q-modification in precursor-tRNAs, which just got published in @natcomms.nature.com.

This was a very exciting and fruitful collaboration with
@francescatuorto.bsky.social in Heidelberg.

www.nature.com/articles/s41...

Thrilled to announce the nexy Austrian Cryo-EM Symposium, taking place 11th-12th November at @istaresearch.bsky.social, with speakers inc. @grigoriefflab.bsky.social, @oliveiramann.bsky.social, @bjgreber.bsky.social @svenklumpe.bsky.social @sebastianglatt.bsky.social ‪‬https://tinyurl.com/cryo2025

Dear all,

We are starting an exciting new project in close collaboration with Clément Carré in Paris, France.

A fully funded PhD position is available in my group at the vetmeduni Vienna!

Please help us spreading the word - please forward it to interested students.

This cover highlights the article Determining the effects of pseudouridine incorporation on human tRNAs by Anna D. Biela, Janusz M. Bujnicki, Ting-Yu Lin, Sebastian Glatt and colleagues. Artistic representation of a human tRNA structure and how the tRNA shape is sculptured by pseudouridine synthases, illustrating the understanding that precise incorporation of modifications is key to increase tRNA stability necessary for protein translation. Scientific illustration by Dominika Dobosz, MCB / Jagiellonian University Krakow

On the cover of issue 13: @sebastianglatt.bsky.social et al
Highlights: circadian clock protein dynamics; inflammatory gut-kidney crosstalk
Resource: EBV-host chromatin interactome
Structural insights into mitochondrial translocation of folded Rieske protein
...
www.embopress.org/toc/14602075...

2nd Polish #RNA Biology Meeting in #Poznan co-organized by @uam-ibmib.bsky.social‬ has started yeaterday evening with talks of Ramesh Pillai & Sebastian Glatt @sebastianglatt.bsky.social‬ - many more great talks, posters & discussions ahead! #PolishRNABiologyMeeting

The benefits of an unusual career path – People – EMBO Meet Sebastian Glatt, Professor of Systems Genetics at the University of Veterinary Medicine in Vienna | EMBO Member and former EMBO Installation Grantee

“EMBO is really a super-active community,” says Sebastian Glatt @sebastianglatt.bsky.social, EMBO Member, former EMBO Installation Grantee and Professor at the University of Veterinary Medicine Vienna #Austria.

Read more here: www.embo.org/people/the-b...
🧪 #LifeSciences #research #opportunities

Thanks Luca!

Many thanks to our collaboration partners in Warsaw at the @iimcb.bsky.social, the fantastic team #MCB, @jagiellonskiuni.bsky.social, #SOLARIS and my new team #Vetmeduni Vienna. We highly acknowledge financial support from @erc.europa.eu

Last but not least, we purified individual human tRNAs directly from human cells and analyzed by single-particle cryo-EM to illustrate the potential of the approach as a future diagnostic tool.

We analyzed the local effects also in our cryo-EM maps and by using a newly developed biophysical method.

The team of @jmbujnicki.bsky.social used MD simulations to analyze the effect of specific Ψ-incorporation at positions 13 and 55 to understand the reasons behind the stabilizing effects on the tRNA molecules.

Our work is the first study to provide structures of several tRNAs before and after the introduction of modifications at specific sites. In detail, we analyzed 4 human tRNA species to decipher structural and stability effects induced by the installation of Ψ at single or multiple sites.

Foremost, our work also demonstrates the feasibility of tracking subtle conformational changes in tRNAs after getting modified. To achieve this, we purified all five human pseudouridine synthases (PUS) and generated tRNAs carrying different modification patterns.

We used single particle cryo-EM to directly visualize several human tRNAs and analyze the effects of individual tRNA modifications. To our knowledge, the work presents interpretable maps of the smallest asymmetric particles analyzed by single-particle cryo-EM so far.

Determining the effects of pseudouridine incorporation on human tRNAs | The EMBO Journal imageimagetRNAs harbour a complex set of modifications which are important for their function. Here, single particle cryo-EM, biophysics, and computational analyses of the effects of pseudouridine inc...

We are very excited about our latest work, which just came out @embojournal.org – www.embopress.org/doi/full/10..... The work @MCB_UJ was driven by Anna Biela and Ting-Yu Lin 林亭妤 in collaboration with @jmbujnicki.bsky.social…a short🧵

tRNAs are small and sport heterogeneous base modifications – yet @sebastianglatt.bsky.social @jmbujnicki.bsky.social Ting-Yu Lin 林亭妤 et al show #cryoEM combined with biophysical & computational approaches can give insights into how #pseudouridine affects human tRNA!
www.embopress.org/doi/full/10....

All Eurpean RNA Researchers: join us at the 2nd Polish RNA Biology Meeting @uam-ibmib.bsky.social,June 24-27 Poznan! Keynote speakers include Elena Conti, Andrzej Dziembowski @sebastianglatt.bsky.social, Reinhard Lührmann, Kai Papenfort, Ramesh Pillai & Bertrand Séraphin! #RNA 2ndrna.web.amu.edu.pl

Austria 🇦🇹congratulates @sebastianglatt.bsky.social who receives the 🇵🇱 FNP Prize 2024 in Life and Earth Sciences – also known as the "Polish Nobel Prize".

His findings open new pathways for research into genetic disorders and potential therapeutic developments.🔬

#FoundationForPolishScience