Maxim Greenberg

Thanks! I had a vision for the art, but unfortunately don't have the talent, so I needed some AI help. The aesthetics of the poster are thanks to @jamiehackett.bsky.social

😅

Amazingggggg

How do the H2A.Z histone variant and its dedicated chromatin remodeller SRCAP regulate gene expression ?
Beautiful work from @armelletollenaere.bsky.social now published @ www.nature.com/articles/s41...

it kind of slaps...

How Is The Dow - Auto-Tune The News, Wherein Pam Bondi Discovers An Unassailable Riposte That Canno YouTube video by schmoyoho

I literally can not see this without thinking of this song now youtu.be/Q71Xb1Sd86M?...

📣 Ctrl + Epi + Edit, Engineering the Epigenome - Abstract submission and registration are open!

📍Institut Jacques Monod
📆 16-17 November

More informations🔗 ctrlepiedit.sciencesconf.org?lang=en

We will have 2 half-day sessions on Nov 16-17 @ijmonod.bsky.social w posters & a keynote from Charlie Gersbach @duke-university.bsky.social. Speakers will be selected from abstracts!
@jamiehackett.bsky.social @ficzlab.bsky.social @c-jake-harris.bsky.social @eddaschulz.bsky.social (2/2)

Flyer for symposium: https://ctrlepiedit.sciencesconf.org/

Very excited to announce the FIRST symposium on epigenome editing! These tools are becoming widely used in mol bio, ag & therapy. It's time to bring leaders together to discuss this rapidly growing and exciting field. And why not in Paris! Please register & share! (1/2) ctrlepiedit.sciencesconf.org

In animals with large genomes, finding cis-regulatory elements can be very challenging. Enhancers can be located tens/hundreds of kb away from their target promoters. We face this challenge in Parhyale, with >3 Gbp genome.
We just published a preprint describing how we are tackling this problem. /1

Very similar to Uncut Gems (the unrelenting anxiety...). I thought the character of Marty made the movie superior

One frame. Many voices. ✨
This International Women’s Day, our Women in Science series features group leader Christa Bücker 🙌

Today, @chribue.bsky.social shares an important perspective on finding role models and becoming one for the next generation 👩‍🔬

@univie.ac.at
@meduniwien.ac.at

I liked it!! But I didn't think it was Yorgos' best. Also, the 4 above were all legitimately great IMO

Too high or too low?

Exactly 0 people care about my opinion on this, but I weirdly (for me) saw a lot of the Oscar nominees this year. This is my ranking:

1) Hamnet
2) OBAA
3) Sinners
4) Marty Supreme
5) Bugonia
6) F1
7) Frankenstein

The first 5 were all awesome. 6 was fun. 7 is hot garbage

Postdoc positions in Nuclear RNA Biology - Vacancy at Aarhus University Vacancy at Department of Molecular Biology and Genetics - RNA Biology and Innovation, Aarhus University

Postdoc positions available in my lab in Aarhus, Denmark on 'Mammalian Nuclear RNA Production and Turnover Systems'. Please get in touch for further information or simply apply here:
mbg.au.dk/en/news-and-...

Figure 1.(A) Classical gel electrophoresis experiments showing mono-, di-, tri-, tetra-, and further multinucleosome bands upon chromatin digestion. (B) The nucleosome repeat length (NRL) is defined as the genomic distance between the centres of two neighbouring nucleosomes.

Figure 2.Nucleosome mapping using MNase-seq versus ATAC-seq. (A) In MNase-seq, nucleosomes in both open and tightly packed genomic regions are accessible to digestion. MNase preferentially cleaves DNA between nucleosomes and digests DNA until it encounters a histone octamer, which provides a footprint of nucleosome-protected DNA regions. (B) Bulk MNase-seq results in averaged maps across millions of cells, effectively capturing all possible nucleosome positioning configurations. (C) Single-cell MNase-seq (scMNase-seq) results in a noisier and sparser signal. The resulting footprints still represent nucleosome-protected regions, but not all nucleosomes are represented. (D) In ATAC-seq, open regions can be accessed by the enzyme Tn5 transposase, which can insert primers in regions free from the binding of nucleosomes and transcription factors (TFs). (E) For open chromatin regions, nucleosome maps can be obtained from ATAC-seq similar to MNase-seq. (F) Closed, tightly packed chromatin regions may be less represented in ATAC-seq nucleosome maps.

Figure 5.Molecular mechanisms affecting nucleosome spacing. (A) Linker histones H1 and nonhistone chromatin proteins which compete with H1s and modulate nucleosome spacing through structural and electrostatic mechanisms. (B) Chromatin remodellers actively reposition nucleosomes following context-dependent rules. (C) Cell state-dependent chromatin boundaries formed by CTCF and other structural proteins, as well as associated recruitment of chromatin remodellers which space nucleosomes. (D) Gene activity associated with remodeller action and RNA polymerases transcribing through the nucleosomes, leading to smaller distances between nucleosomes in regulatory regions and gene bodies. (E) DNA sequence repeats of different types.

Figure 6. Examples of NRL changes in biological systems. (A) Cell differentiation leads to NRL changes between different cell types, e.g. mouse dorsal root ganglia neurons (NRL ∼165 bp) versus cortical astrocytes (NRL ∼183 bp) [175]. Schematic cell shapes are adapted from an image created in BioRender (https://BioRender.com/89trj2t). (B) Paired normal versus tumour breast tissues show NRL shortening in cancer (figure adapted from [36] under the CC BY 4.0 licence (https://creativecommons.org/licenses/by/4.0/)). (C) Nucleosome positioning derived from cfDNA of human volunteers shows NRL increase with age (figure reprinted from [79] under the CC BY 4.0 licence (https://creativecommons.org/licenses/by/4.0/)).

Nucleosome aficionados! Our new review "Nucleosome spacing across cell types, diseases, and ages" is out in NAR: academic.oup.com/nar/article/...

A huge effort to pull together what we’ve learned about nucleosome spacing in many systems. Enjoy!
@milena-bikova.bsky.social @chrsclrksn.bsky.social

If you want to explore ncRNA dynamics during mammalian development, love microscopy, and are not afaraid of transposons biology, apply‼️
We are looking for a postdoc to join our team at @imbavienna.bsky.social
More details 👇🏻

Our Lab is searching a postdoc for an exciting ERC-funded project that will start this summer. Apply if you’re passionate about multi-omics, cutting-edge computational approaches, and exploring biological questions across the animal 🪼 🪱 🐞 🦎 🦋 🕷️ tree of life www.biozentrum.unibas.ch/open-positio...

A reminder that we are actively recruiting a geneticist to the Department of Genetics at Albert Einstein College of Medicine

careers-einstein.icims.com/jobs/17847/a...

Post-replicative chromatin accessibility predicts cell fate change Knudsen and colleagues use repli-ATAC-seq to compare replicated and unreplicated chromatin in two models of cell identity change. They find that lineage-specific elements are accessible earlier in rep...

DNA replication globally disrupts the epigenome. But does this create a chromatin-access opportunity for TF binding to facilitate cell identity change? Now 𝐫𝐞𝐩𝐥𝐢-𝐀𝐓𝐀𝐂-𝐬𝐞𝐪 shows 𝘥𝘦 𝘯𝘰𝘷𝘰 chromatin opening & TF binding occurs specifically post-replication, in cellular differentiation & reprogramming!

I am excited to offer a postdoctoral research position in my lab in Oxford studying the distribution of small RNAs during plant sexual reproduction. shorturl.at/7Goxt
Reposts appreciated!
Obligatory cat video to brighten your day. :-)

Considering Paris is A) well known for its food and drink and B) is the most touristed city in the world, the food and drink options at Paris airports are shameful (yes I’m bored at the airport right now…)

Sad to miss it :-(

This will be a great meeting! Don't forget to register!

📢 Institut Jacques Monod Seminar

📅 February 27th
📍 Institut Jacques Monod

Invited by the @ishier.bsky.social, @gautamdey.bsky.social will present the Institut Jacques Monod seminar "Evolution and diversity of mitosis"

➡️ buff.ly/uCSfjgQ

Excited to share @suminkim.bsky.social and @mileshuseyin.bsky.social 's new Current Opinion review on how Polycomb complexes mediate 3D genome interactions including mechanistic models and potential roles in gene regulation:
www.sciencedirect.com/science/arti...

Ancient co-option of LTR retrotransposons as yeast centromeres - Nature Evolutionarily related ‘proto-point’ centromeres providing resolution to the evolutionary origins of point centromeres are identified in yeast, and comparison shows they evolved in an ancestor with re...

Our paper is now out in Nature:

“Ancient co-option of LTR retrotransposons as yeast centromeres”

www.nature.com/articles/s41...

A short thread on how retrotransposons helped give rise to yeast point centromeres.

1/14

A pilot in a submersible vehicle collecting sediments samples in 30 meters of water looking for Asgards (microbial relatives of eukaryotes)

One of the biggest questions in biology is how complex cells evolved about 2 billion years ago. Here's my new story on how scientists are solving the mystery of eukaryotes like us. Gift link: nyti.ms/4qMbo22

We are delighted to launch the (free!) registration for our 5th AICC symposium, hosted this year in @ucddublin.bsky.social on May 27th.

If you are interested in chromatin, gene regulation or the nucleus please join us!

allirelandchromatinconsortium.ie/annual-meeti...