Excited to share our new preprint from the Klose lab, where we investigate how SET1/MLL complexes shape H3K4me3 and regulate transcription in mouse ES cells. www.biorxiv.org/content/10.6...
An early Christmas present for those interested in chromatin and transcription! Fantastic work from @au-ho-yu.bsky.social and @aleksszczurek.bsky.social . Thanks to Inge and Michiel for their help. Please repost!
www.biorxiv.org/content/10.6...
Yes, the buffer that comes with Terra polymerase is always the limiting factor. We have a massive excess of Terra polymerase itself, but unfortunately it doesn’t seem to work with our regular PCR buffer! Would love to know if anyone has managed to get it working well in another buffer!
We have had multiple discussions about why people have not previously observed the very dramatic reduction in transcription upon PNUTS depletion. So I looked at our own TT-seq data and analysed it without including the spike-in. The numbers speak for themselves! Spike-in calibration is important!
We don’t think the impact factor is a good thing. But this is not the fight we want right now. We are trying something amazing and it’s working. We don’t want authors or other journals to fear trying it. Clarivate know they have this control and are using it to squash innovation. It is bullying.
Latest from the Klose lab: we discover a new role for the PNUTS-PP1 phosphatase complex in regulation of PolII pause release. www.cell.com/molecular-ce...
Fantastic to see the latest story from the Klose lab out in Molecular Cell today! Thoroughly enjoyed being part of a team effort spearheaded by @jesskelley.bsky.social and @edimitrova.bsky.social! A project that briefly dragged us away from CpG islands before leading us back to the 5’ end of genes!
