In future work, I am now using our multispectral technology to study membrane trafficking pathways in cytotoxic T cells and related immunotherapies, to decode how T cells are able to eliminate virally-infected/cancer cells and what happens in patients when these processes go wrong. Updates to come!
We demonstrated the ability to simultaneously image trafficking of five different endogenous receptor classes using de-novo binders from David Baker's lab, by adding the multispectral technology to a state-of-the-art single objective light sheet microscope, capturing data at 3 volumes per second.
The technology we developed is compatible with any camera-based microscope and by using polycistronic plasmids and live-cell dyes (e.g. @spirochrome.com) we can image multiple organelles in live cells simultaneously.
🚨Publication alert🚨
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:
Today's light sheet video of actin dynamics in cytotoxic t cells... so much fun to come!
Thank you very much for the feature and the article!! A nice surprise before the weekend...
Congratulations @lara-kruger.bsky.social ! We wish you all the best!
🚨 Paper Alert 🚨
Our preprint describing Elongator as a selective microtubule polymerase that rewrites PTMs on microtubules is now published! Check it out: www.embopress.org/doi/full/10....
Picture of Julie wearing a red top and black bottoms, holding a bottle of champagne
Congrats to @juliemiesch.bsky.social who was awarded a Postdoc Mobility grant by @snf-ch.bsky.social to support her research for two years. Her postdoc research will focus on the biophysical regulation of water buffering in the cell.
Mitochondria consist of networks of cylindrical tubes, right? Not necessarily! - in our new preprint, @gavsturm.bsky.social investigates how mitochondria transiently adopt a beads-on-a-string morphology
www.biorxiv.org/content/10.1...
Akaash Kumar stands next to his multispectral imaging hardware which features several cameras. Behind him, a computer screen shows a colourful cellular image captured with his new microscopy system.
Congrats to @akaashkumar.bsky.social who has won the PhD Award from the Cambridge Centre for Physical Biology for his work developing a novel microscopy system with @deriverylab.bsky.social & @jamesdmanton.bsky.social in @cellbiol-mrclmb.bsky.social.
www.physbiol.cam.ac.uk/akaash-kumar
#LMBintheNews
Simultaneous multispectral imaging of seven organelles in live cells using our multispectral spinning disk confocal microscope! #FluorescenceFriday
You can read more about this technology in our updated pre-print: www.biorxiv.org/content/10.1...
Thank you!
Very happy that my work on developing multispectral live cell imaging technology has been recognised by the Cambridge Centre for Physical Biology! Thanks for the award!
Hi Mike, @dmikeando.bsky.social could I be added to this please?
In June, @akaashkumar.bsky.social and @jamesdmanton.bsky.social had their Multispectral live-cell imaging paper released on to the BioRxiv www.biorxiv.org/content/10.1...
Akaash stands in front of some stairs, holding a bottle of champagne whilst wearing a suit.
Then, in September @akaashkumar.bsky.social passed his PhD viva, becoming the latest doctor to emerge from the lab! He then won the RMS Early Career Award 2024 in October.
On the bottom left is Akaash Kumar, bottom right is Lara Kruger, behind lara is Manu Derivery and behind Akaash is James Manton, all holding glasses of champagne.
Later in October, the LMB had their yearly annual symposium with Post-doc Lara Kruger winning the Steitz Prize for her talk and @akaashkumar.bsky.social winning the Perutz Prize for his PhD work!
Some personal news: my wife and I are finally homeowners! We got the keys to our home in Cambridge today, very exciting times!
Likewise! Took me a while aha hope you're well!
Would love to be added to this, for all things multispectral microscopy
Aha thank you very much!
Would love to be added. Many updates on all things multispectral live cell imaging!
Thanks for this, super useful for new joiners to bluesky (like me)! Could I be added to this group please?
Hello Bluesky! I am an engineer/scientist currently working at the MRC LMB in Cambridge UK. During my PhD I developed new imaging technologies to shed light on the dynamics of living cells e.g. here is a movie of 6 organelles in live human cancer cells. Follow for more updates! #science #biology
