Sophie Janssens Lab

Great news, Jan! Well deserved!

😂 there have to be rules for everything😉. Thanks, Caetano, it was an honour (and pleasure) to be evaluated by you and a great panel of top scientists!

Thanks to @viblifesciences.bsky.social and @ugent-fge.bsky.social for their support and all VIB Cores, especially @singlecellcore.bsky.social for their tremendous help. Enjoy the reading!

A publicly available web-interface can be used to calculate the maturation score of your mature DCs of interest.
www.single-cell.be/Spleen_cDC1_...

A meta-analysis confirms that homeostatic and immunogenic mature signatures are conserved across tissues and DC subsets, supporting a unified nomenclature for mature DCs.

Our study revealed that empty LNPs do not induce immunogenic maturation of DCs, while mRNA-containing lipids do, showing that the cargo, not the lipids, determine adjuvanticity.

By using an approach relying on the induction of homeostatic DC maturation by uptake of empty LNPs and immunogenic DC maturation by TLR ligand-adjuvanted LNPs, and performing CITE-SEQ, a flow cytometry panel was designed to assign mature DCs as immunogenic or homeostatic.

Lipid nanoparticles as a tool to dissect dendritic cell maturation pathways Rennen et al. provide methods to distinguish homeostatic from immunogenic mature cDC1s, based on lipid nanoparticle (LNP)-induced maturation. Only the uptake of TLR ligand- or mRNA-encapsulated LNPs i...

In the new Resource paper from Sofie Rennen and @victor-bosteels.bsky.social, published in @cp-cellreports.bsky.social, they present a method to reliably annotate them. www.cell.com/cell-reports...

Ever wondered how to deconvolute homeostatic from immunogenic mature dendritic cells by flow cytometry or by using transcriptional profiling?