Maja Radulovic

LLOMe has long been used to study lysosomal damage, yet how it works has remained a mystery.
Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes – revealing a new paradigm for lysosomal failure.

🔗 doi.org/10.64898/202...

#CryoET

Sense, plug, and seal: proteins as both rapid responders and constitutive barriers supporting organelle compartmentalization | Molecular Biology of the Cell Although organellar compartmentalization is primarily established by the delimiting phospholipid bilayer membranes, the contribution of proteins has been less appreciated. Recently, studies across man...

doi.org/10.1091/mbc....

α-Synuclein aggregates inhibit ESCRT-III through sequestration and collateral degradation

In a new publication, Team Harper found that α-synuclein fibrils cause ESCRT-III loss through “collateral degradation,” weakening endolysosomes and promoting further aggregation🔬

🔗 Read the full #publication: bit.ly/4iPUayj

Damage done, tubules on - Studying membrane damaged-induced lysosomal tubulation  - FocalPlane Damage done, tubules on - Studying membrane damaged-induced lysosomal tubulation  - Default

Grateful to @focalplane.bsky.social for inviting us to write about our last paper on lysosomal tubulation during the stress response (rupress.org/jcb/article/...)!

focalplane.biologists.com/2025/12/11/d...

Autolamellasomes: An Autophagy-Dependent Parallel Pathway for Bulk ER Degradation and Lysosome Biogenesis https://www.biorxiv.org/content/10.1101/2025.11.16.688694v1

Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.

For more details and to apply please see

www.imperial.ac.uk/jobs/search-...

Lipid Landscape of human cells https://www.biorxiv.org/content/10.1101/2025.10.05.680593v1

VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repair https://www.biorxiv.org/content/10.1101/2025.10.23.684214v1

🚨 We’re hiring a postdoc! 🚨
Interested in lysosomes, Parkinson’s disease, cell biology, microscopy — or all of the above? Come work with us at the Bonet-Ponce Lab!
Reach out directly: luis.bonetponce@osumc.edu
Learn more: bonetponcelab.com
Let’s chat!

Towards a unified framework for the function of endoplasmic reticulum exit sites - Nature Reviews Molecular Cell Biology Endoplasmic reticulum exit sites (ERES) are specialized ER subdomains that regulate the export of secreted cargo. This Roadmap explores how ERES integrate biochemical and mechanical signals to coordinate trafficking and proposes a multidisciplinary strategy to investigate their function, including in disease.

ER exit sites are dynamic ER subdomains that integrate signals, reshape membranes and steer secretion. Twelve groups in the field came together to map their diversity, regulation, unanswered questions and future directions.
www.nature.com/articles/s41...

Much needed good news!! Our paper is just out in @jcb.org. We found a crazy antagonistic motor function that explains the elongation/retraction phenotype we see during LRRK2-dependent lysosomal tubulation. I am especially proud of Tsion, Nuria, Mia and Irene's contribution. Check it out!

CHIP protects lysosomes from CLN4 mutant-induced membrane damage Nature Cell Biology - Lee et al. use an aggregation-prone CLN4 mutant that causes lysosomal damage in neurons and show that in non-neurons, the ubiquitin ligase CHIP prevents CLN4-dependent...

Happy to share an exciting study from Yihong Ye’s lab at NIH, with a minor contribution from our lab: ceroid lipofuscinosis-4 (CLN4)-linked DNAJC5 mutations cause lysosomal damage as a driver of neurodegeneration in iPSC-derived neurons. CHIP safeguards lysosomes via microautophagy 👉 rdcu.be/eChof

LYVAC/PDZD8 is a lysosomal vacuolator Lysosomal vacuolation is commonly found in many pathophysiological conditions, but its molecular mechanisms and functions remain largely unknown. Here, we show that the endoplasmic reticulum (ER)–anch...

LYVAC/PDZD8 is a lysosomal vacuolator | Science www.science.org/doi/10.1126/...

Quantitative imaging of lipid transport in mammalian cells - Nature Directional, non-vesicular lipid transport is responsible for fast, species-selective lipid sorting into organelle membranes.

Nature research paper: Quantitative imaging of lipid transport in mammalian cells

go.nature.com/4mmSygd

Thank you so much! It goes both ways :-)

Interested in lysosome damage and repair pathways? Check out the latest updates.

Intracellular mechanics and organelle mechanobiology Mechanobiology is an interdisciplinary field that emerges at the cross-section of biology, physics and engineering. It aims to understand how living cells, tissues and animals sense and respond to me…

Announcing a new @embo.org Workshop on "Intracellular mechanics and organelle mechanobiology" that we are organizing with Michael Krieg and Verena Ruprecht at @icfo.eu (Barcelona) on 16-20 February, 2026. Please, repost and spread the word! 🙏 #EMBOmechanobio

meetings.embo.org/event/26-org...

What a fantastic ESCRT meeting, the field is going strong and full of exciting science. Many thanks to @rouxlab.bsky.social @cellarchlab.com @jujumathieu.bsky.social @magromayor.bsky.social @majarad.bsky.social for organising such a a great event

The expanding repertoire of ESCRT functions in cell biology and disease - Nature This Review examines recently gained insights into the roles of ESCRT complexes in viral infection, immunity, cancer and neurological disease.

The ESCRT complexes are a fascinating membrane cutting machine with roles in countless cell pathways, but they are also ubiquitous in disease and offer new opportunities as drug targets. See our new review with Alyssa Coyne, Marta Miączyńska, and Harald Stenmark at tinyurl.com/yex3trem

ESCRT function, evolutionary diversity and mechanism of action The ESCRTs (Endosomal Sorting Complexes Required for Transport) were discovered in 2002 and became one of the most dynamic fields of research in membrane cell biology. ESCRTs are protein complexes fo…

A short reminder that registrations for the EMBO ESCRT meeting 2025 in Montreux June 23rd-June27th are still opened! Please register online at meetings.embo.org/event/25-escrt

Amazing! Can't wait to read it in detail. Congratulations!