Emmanuele Severi

Thanks for sharing! Summary of the manuscript in the thread: bsky.app/profile/arit...

Their applications were consistently rejected as “of no interest”/“not translatable” and variations thereof.

Now to enter field does actually requires one to find their niche. 2/2.

Resurgence in phage research is relatively recent. When I first moved to the field in 2010, we still relied on publications from the 70s-80s-90s. Now everything is being rediscovered.

Friend of mine tried for years back in the 00s to have a grant funded to do structural biology of phage. 1/2

#microsky #phagesky

On #phage T4:

www.biorxiv.org/content/10.6...

The metabolome of an E. coli CRISPRi library identifies benefits of minimal metabolite levels and targets for engineering Rapp et al. combine genome-scale CRISPRi and metabolomics in E. coli. CRISPRi of enzymes enforces metabolite accumulation, which leads to regulatory crosstalk and side reactions. This suggests that ma...

#microsky #synbiosky

www.cell.com/cell-systems...

#microsky #synbiosky

Transporters matter!

www.nature.com/articles/s41...

TBH I can’t begin to imagine how many valuable samples have been lost through people moving on and/or retiring. Especially legacy material that is hard to source these days (strains, plasmids, phages).

The International Conference on Retinal Proteins (ICRP2026) registration and abstract submission are now opened!!! www.congressi.unisi.it/retinalprote...

#microsky #phagesky #phage defence

www.biorxiv.org/content/10.6...

Convergent MurJ flippase inhibition by phage lysis proteins - Nature A common mechanism of inhibition of the essential lipid II flippase MurJ by three distinct phage-encoded single-gene lysis proteins provides insights into potential new targets for antimicrobial devel...

#phagesky #microsky

RNA #phage s synthesise inhibitors of the membrane protein MurJ.

(I might be late to the party, but wanted to post it anyway)

www.nature.com/articles/s41...

An Engineered Variant of E. coli Nissle 1917 with Enhanced Transformation Efficiency and Robustness The gut microbiome and its effects on human health have generated considerable scientific, veterinary, and medical interest in recent years. Several gut bacterial species have emerged as potential cha...

#microsky #synbiosky #microbiomesky

pubs.acs.org/doi/10.1021/...

🧵 Proud to present a tour de force by postdoc @gregbwhitfield.bsky.social solving the mystery of how bacterial Tad pili can extend and retract with a single motor ATPase. Great collaboration with Lynne Howell, @dr-lori-burrows.bsky.social, @ianyyen.bsky.social www.biorxiv.org/content/10.6...

Fungal-derived cellobiose metabolic pathway fuels T cells to bypass intratumoral glucose competition Solid tumors harbor immunosuppressive microenvironments that inhibit tumor-infiltrating lymphocytes (TILs) through the voracious consumption of glucos…

"Nobody will care about your work about sugar metabolism and transporters in Neurospora crassa"

Nobody can predict what can happen with your work.

www.sciencedirect.com/science/arti...

CH•••S hydrogen bonds drive molecular recognition of ergothioneine by the microbial transporter Alkyl CH•••S hydrogen bonding interactions enable selective transport of the antioxidant ergothioneine into bacterial cells.

#microsky

Transporters matter and they’re always fun to work with

www.science.org/doi/10.1126/...

#microsky #phagesky #phage #MGE

Prophages found in conjugative megaplasmids from gut Bacillota

www.biorxiv.org/content/10.6...

A One Health study of Klebsiella pneumoniae species complex plasmids shows a highly diverse and ecologically adaptable plasmidome Plasmids play a pivotal role in the horizontal gene transfer (HGT) of antimicrobial resistance (AMR) and virulence determinants among bacteria. Members of the Klebsiella pneumoniae species complex (Kp...

Fascinating and important study on Klebsiella plasmid diversity

'Plasmids from human, animal and marine [Klebsiella pneumoniae complex] isolates formed shared genetic clusters spanning ecological boundaries'

www.microbiologyresearch.org/content/jour...

Streptomyces and other actinos are really fascinating bacteria. Always something new to discover.

Rapid directed evolution guided by protein language models and epistatic interactions Protein engineering is limited by the inefficient search through a high-dimensional sequence space to find combinations of synergistic mutations. Traditional approaches use stepwise mutation stacking,...

#synbio #synbiosky

www.science.org/doi/10.1126/...

Structure and mechanism of antiphage retron Eco2 - Nature Structural & Molecular Biology This study shows how the bacterial retron Eco2 defends against viruses. Phage nucleases trigger activation of Eco2, which cuts RNAs, shuts down protein production and stops phage replication.

#microsky #phagesky #phage defence

www.nature.com/articles/s41...

#microsky #microbiomesky #glycotime

Akkermansia FTW

www.biorxiv.org/content/10.6...

The February issue is live nature.com/nbt/volumes/...

Barcoding microbial ribosomal RNA creates a record of gene transfer events without requiring translation. Kalvapalle et al. designed catalytic RNAs to barcode host RNA upon gene transfer, identifying the community involved go.nature.com/440gL5E

#microsky #phagesky

Prophages #phage from Acinetobacter baumannii

www.biorxiv.org/content/10.6...

Ah, a nice continuation of their previous Paenibacillus-mediated bioconversion of Pseudomonas lipopeptide (which I already like a lot); now, characterization of enzymes behind the process

👏👏👏🎉

Delighted to see our work on the impact of drought on the oak microbiome featured on the cover of @cp-cellhostmicrobe.bsky.social
Link to the article below this post 👇🏻

What a superb story from @jamesemcdonald.bsky.social and team in @cp-cellhostmicrobe.bsky.social👏

144 Quercus petraea trees subjected to drought, nutrient stress m, and biotic treatments (bacterial pathogens and beetle larvae) to simulate acute oak decline➡️ microbial communities over 2 years🌳

#microsky #glycotime #glycotimesky

www.biorxiv.org/content/10.6...

Sialic acid-responsive Parabacteroides is linked to gut barrier integrity in older adults Aging is frequently accompanied by inflammaging—a chronic, low-grade inflammatory state that contributes to functional decline and disease risk. Disruption of the intestinal barrier is increasingly...

#microsky #glycotime #sialic acid

www.tandfonline.com/doi/full/10....

Animal-associated jumbo phages as widespread and active modulators of gut microbiome ecology and metabolism A widely distributed but overlooked large-sized phage clade is active in human gut and thus could potentially affect human health.

#microsky #phagesky

Jumbo #phage from the gut - “Jug”

www.science.org/doi/10.1126/...

#microsky #phagesky

Structures of PhiC31’s integrase with/without its RDF 👏

Surely @factor50.bsky.social knows about/will like it ;-)

www.biorxiv.org/content/10.1...