"HLA-independent T cell (HIT) receptor (26), which substitutes the antigen recognition domain of a T cell receptor (TCR) with that of a CAR, thereby co-opting the downstream signaling afforded by the CD3 complex"
www.science.org/doi/10.1126/...
"Assembloids ... are fusion structures that can combine multiple organoid types for the purpose of accounting for inter-regional interactions or can even incorporate missing cell types within the same organoids."
"Organoids, also referred to as βmini-organsβ, are three-dimensional (3D) in vitro grown structures exhibiting either complete or partial resemblance to in vivo organs in terms of cellular organization, microanatomy and architecture."
pmc.ncbi.nlm.nih.gov/articles/PMC...
iCLAP: integrable Co-detection of Low-Abundant Proteins.
"enables sensitive and highly multiplexed protein detection within the same FFPE tissue section"
www.nature.com/articles/s41...
4i: iterative indirect immunofluorescence imaging.
www.science.org/doi/10.1126/...
IMC: imaging mass cytometry.
"coupled immunohistochemical and immunocytochemical methods with high-resolution laser ablation to CyTOF mass cytometry."
www.nature.com/articles/nme...
t-CyCIF: tissue-based cyclic immunofluorescence.
"conventional low-plex fluorescence images are repeatedly collected from the same sample and then assembled into a high-dimensional representation."
elifesciences.org/articles/31657
CODEX: CO-Detection by indEXing.
Multiplexed DNA-tagged antibody staining.
www.cell.com/cell/fulltex...
There are two nonavalent HPV vaccines, one approved by the FDA in the USA (2014), the other one approved by the NMPA of China (2025).
www.mdpi.com/2076-393X/14...
"hypoxia correlates with immune cell state and organization within granulomas and might subvert immunity to TB"
www.nature.com/articles/s41...
I only knew the transwell assay and the wound healing assay.
"The median age of patients with HAdV infection was 3 years, with infants aged 0-2 years accounting for the majority (41.39 %)."
www.sciencedirect.com/science/arti...
βintranasal **DNA vaccine** fusing the Mtb ... relMtb with the gene encoding the dendritic cell-targeting chemokine Mip3a/CCL20β
JCI - Immunotherapy targeting drug-tolerant Mycobacterium tuberculosis persisters accelerates tuberculosis cure in preclinical models www.jci.org/articles/vie...
Furthermore, the ratio of activated (CD38+ HLA-DR+) versus exhausted (TIM-3+PD-1+) CD8+ T cells was higher in the early ToD group (Pβ<β0.001) compared with the late ToD group (Pβ<β0.001).
"Over the first four cycles (of immunotherapies), morning circulating CD8+ T cells increased in the early ToD (time of day, before 15:00) group, whereas they declined in the late ToD (after 15:00) group (Pβ<β0.001)."
TIL:
mtcars |> _$cyl
"x" |> assign(1)
"Human cells and bacteria produce more than 250,000 molecules, or metabolites, as they grow and survive.
**Some of these** can enter the bloodstream, circulate to the lungs and diffuse into exhaled air."
What your breath says about the bacteria in your gut www.nature.com/articles/d41...
One protocol of dual RNA-seq (i.e. host zebrafish and pathogenic Mycobacterium marinum)
pubmed.ncbi.nlm.nih.gov/41564181/
β**whole new dimension of genetically encoded magnetic sensors and actuators**β
β... had make a remote-controlled antibody, which it calls MagBodies, the binding strength of which can be controlled by a magnetβ
βMagLOV's dimming was due to an effect in which the quantum properties of an electron pair in the protein could be altered by a magnetic fieldβ
βfurther improvements (of MagLOV or other fluorescent proteins) should be possibleβ
GFP dims in the presence of a weak magnet (about 1%).
"... engineered a more responsive protein, called MagLOV, the fluorescence of which dims by half or more in the presence of a magnet"
www.nature.com/articles/d41...
The Shingles vaccine has outperformed all expectations. Why?
erictopol.substack.com/p/spotlight-...
"Importantly, ... exploiting distinct properties of a single object for different functions.
Comparable behavior has only been consistently documented in chimpanzees."
Flexible use of a multi-purpose tool by a cow: Current Biology www.cell.com/current-biol...
inserting GSDMDNT sequence along with a heat shock protein (HSP) promoter into OVs (termed GOV), enabling rapid, continuous viral replication through pyroptosis by GSDMDNT expression.
"GOVs were loaded into these donor cells, which were treated with cytochalasin B (CB) and extruded to generate iNV-GOVs"
"Donor cells additionally overexpressed CARs targeting HER2, PSCA, Claudin18.2 or GPC3."
"Using CRISPR-Cas9, we knocked out Ξ²2-microglobulin (B2M) and the class II transactivator (CIITA) to eliminate HLA-I/II presentation."
"inserted HLA-E and CD47 by homology-directed repair to protect against natural killer cell killing and macrophage phagocytosis."
www.nature.com/articles/s43...
"Thus, the robust efficacy of IV-BCG in SIV+ MCM is retained regardless of anti-BCG drug treatment, indicating that BCG does not need to survive long to provide protection."
"In contrast, SIV control was necessary for full protection induced by IV-BCG."
pubmed.ncbi.nlm.nih.gov/41510269/
Multiple pathways can lead to tuberculosis (TB) disease from opposing immunological extremes.
Anti-PD-1 therapy (almost exclusively in cancer patients), T2D, malnutrition, HIV, some genetic mutations (MSMD), anti-TNF, and more can lead to tuberculosis.
elifesciences.org/articles/108...
