Guillaume Chesneau

Curious about how the metabolic decoy strategy can reduce fungal pathogenic colonization and protect plants? Visit poster P-236 this afternoon at #2025ISMPMI

Trichomes on an Arabidopsis thaliana leaf (here shown using electron microscopy) are formed by a single cell adopting this spiked shape (top images). Arabidopsis plants without a SCAR/WAVE gene cannot form properly shaped trichomes, because their inner cytoskeleton control is impaired (bottom images). Images by Sabine Brumm.

@binebrumm.bsky.social, together with @dromius.bsky.social team colleagues, unveiled how key proteins act as 'sculptors' in plant cells, taking on different roles to shape development

Read more www.slcu.cam.ac.uk/news/plant-c...
And full paper doi.org/10.1126/scia...

Functional divergence of plant SCAR/WAVE proteins is determined by intrinsically disordered regions Intrinsically disordered regions in SCAR/WAVE proteins drive diverse functions in root hair and leaf trichome development.

Excited to share that our preprint has found a home in Science Advances. We show that intrinsically disordered regions determine the functional diversification of actin-regulatory SCAR proteins in Medicago. 🧵 1/3
www.science.org/doi/full/10....
#ScienceAdvances #PlantScience #IDRs

MetaFlowTrain: a highly parallelized and modular fluidic system for studying exometabolite-mediated inter-organismal interactions - Nature Communications Metabolic interactions shape ecosystems but remain hard to study. Here, authors present MetaFlowTrain, a modular, low-cost fluidic system controlling metabolic fluxes between microbes, and show its va...

📜 MetaFlowTrain: a highly parallelized and modular fluidic system for studying exometabolite-mediated inter-organismal interactions

🧑‍🔬 @chesneau-g.bsky.social, @hacquardst.bsky.social, et al.

📔 @naturecomms.bsky.social

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #PlantImmunity #PlantMethods

5/5. MetaFlowTrain confirms that specific bacterial exometabolites, not media depletion, drive microbial antagonism.
🦠 Pseudomonas R401 WT suppresses SynCom growth via known inhibitory exometabolites.
❌ A triple mutant loses inhibitory power.

4/5. MetaFlowTrain dissects inter-kingdom microbial interactions via directional exometabolite flow.
🦠 Bacterial SynComs strongly inhibit fungal growth, even without physical contact.
🚫 Up to 82% growth suppression via exometabolites.

3/5. MetaFlowTrain reveals how soil conditioning alters microbial assembly/exometabolite output.
🦠 SynComs exposed to plant-conditioned peat undergo major community shifts & consume key metabolites (α-KG, fumarate).
🌱 BF SynCom-derived exometabolites impact Arabidopsis germination & root growth.

2/5. MetaFlowTrain cultivates diverse microbes & collects their exometabolites under continuous nutrient flow.
🌱 Using sterile peat extract, we grew 11 bacterial & 4 fungal strains from the Arabidopsis root microbiota.
💥 Exometabolite profiles differed significantly by strain.

📝 Paper: www.nature.com/articles/s41...
1/5. 🚂 MetaFlowTrain = 4 modular components:
a. Variety of liquid inputs
b. 24-channel pump enabling parallel metabolic trains
c. 3D-printed gnotobiotic microchambers
d. Multi-layered outputs for cell/metabolite analysis
🔗 protocols.io/view/36wgqd68ovk5/v3

MetaFlowTrain : A Highly-Parallelized and Modular Fluidic System. YouTube video by Guillaume Chesneau

🚨Excited to share our new publication! MetaFlowTrain, a highly parallelized and modular fluidic system to study exometabolite interactions between microbes-microbes-plants 🌱
@hacquardst.bsky.social @herpell.bsky.social S.Wolf S.Perin @mpipz.bsky.social
www.youtube.com/watch?v=gNUf...
🧵Thread 5/5:

Our manuscript describing MYCroplanters, a high-throughput Arabidopsis phenotyping tool is out today in Plos Pathogens from first authors Melissa Chen and Leah Fulton! journals.plos.org/plospathogen...

Grateful to the #RPB2025 community for the inspiring scientific exchanges and to IRHS-Angers for organizing such a fantastic 17th Plant-Bacteria Interaction Meeting 🌱🦠. Honored to have been invited to present my work in this incredible setting! ⛰️❄️

First paper of our lab today @natureportfolio.bsky.social by Nussi!

We provide a real-time movie on how #transcription and #translation cooperate using #single-molecule FM: we find long-range #ribosome/ RNAP communication mediated by #RNA looping!

@embl.org #RNAbiology #RNASky

t.co/a24Xxcxdo0

Soil microbiomes show consistent and predictable responses to extreme events - Nature Soils from 30 grasslands across Europe were subjected to 4 contrasting extreme climatic events under drought, flood, freezing and heat conditions, with the results suggesting that soil microbiomes fro...

It’s out!! We subjected soils from 30 different locations across Europe to extreme events and found that soil fungal and bacterial communities showed consistent responses that could be predicted from their origin! With @knightjar.bsky.social and many collaborators!
www.nature.com/articles/s41...