Tee Research Lab

Aldolase a coordinates macropinocytic nutrient scavenging and lysosomal degradation in lung cancer by interacting with V-ATPase - Cell Communication and Signaling Background Oncogenic KRAS mutations in cancer cells drive macropinocytosis, a pathway that scavenges extracellular proteins to obtain nutrients under metabolic stress. Lysosomal degradation is essenti...

Incidentally, #new data showing #aldolase interacts with V-ATPase to increase #acidification and #macropinocytosis 🧪🫧 shorturl.at/b6tJL
@SpringerNature

@BioMedCentral
#moonlighting

Paper here shorturl.at/bhNzw
@MolMetab

Super interesting read : In Aldob-KO models, inhibiting lipogenesis reduces hepatic TGs but does not rescue the fasting phenotype. 🧬⬇️
Points to a defect beyond lipids: impaired nutrient sensing /signaling, not metabolism alone.
#HFI #Metabolism #LiverDisease #NutrientSensing

Single–cell Raman imaging reveals fructose impairs brown adipocyte differentiation Brown adipose tissue (BAT) plays a pivotal role in energy expenditure and metabolic health, yet the influence of specific sugars like fructose on brow…

www.sciencedirect.com/science/arti...

New study with #JHU! Single-cell Raman imaging 🔬 shows fructose impairs brown adipocyte differentiation 🧬, leading to higher lipid unsaturation + esterification and stalled maturation. Glucose drives healthier BAT development. ⚡️
#RamanImaging #Adipogenesis #Metabolism #Fructose #BAT #SingleCell

Sweet Science: Exploring the Impact of Fructose and Glucose on Brown Adipocyte Differentiation Using Optical Diffraction Tomography The thermogenic capacity of brown adipose tissue (BAT) has garnered much attention for its potential to regulate systemic energy balance. BAT depot size and function need to be tightly regulated to p...

🚨 New from our lab!
We used optical diffraction tomography to visualize how glucose and fructose shape human brown adipocyte differentiation — revealing that sugar composition, not just presence, drives lipid buildup and cell morphology.
🧬 Read: doi.org/10.1002/jcb....

Hi there @biddybdev.bsky.social ! Can you DM me to talk about a potential project? I can't seem to DM you on here, thanks!

Establishing Immortalized Brown and White Preadipocyte Cell Lines from Young and Aged Mice Studying adipogenesis and adipocyte biology requires the isolation of primary preadipocytes from adipose tissues. However, primary preadipocytes have a limited lifespan, can only undergo a finite num...

Read more about how we do this here! currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1...

Stem cells isolated from aged mice can be reprogrammed to become adipose (fat) cells! 🧬 In this image, a red dye highlights fat droplets within a cell, while the blue dye marks DNA in the nucleus. A glimpse into cellular transformation!
#lipids #metabolism #adipocyte #aging #stemcells

Photo of a Black man in work clothes harvesting sugar cane - very tall brown stalks with green leafy tops - with a machete

As promised, a (maybe long!) 🧵 on the history of sugar:

1/ Sugar cane began to grow wild in India and south-east Asia about a hundred million years ago, when grasses evolved from earlier plants.

Oops - @bsky.app does not support #gif yet, so here's a video instead!

Hot off the microscope! A metabolic enzyme, tagged with green fluorescent protein shows dynamic movement in a liver cancer cell! #metabolism #enzyme #microscopy

Thank you so much @prideandproud.bsky.social !

FEBS Press Stem cells rely on nutrients and oxygen, which vary by organ, affecting their growth and differentiation. This study explored how sugar, and oxygen levels, impact stem cells. Fructose stresses stem c...

Here is the link to our paper febs.onlinelibrary.wiley.com/doi/full/10....

Excited to share our latest findings on how nutrients affect stem cell behavior! Replacing glucose with fructose induces stress and fails to induce adipocyte differentiation. Instead, it prompts release of senescence-associated cytokines. #StemCells #Metabolism #Adipogenesis #Cytokines