T3SS

The spaO gene produces two variants (SpaOLarge and SpaOshort) that cooperate to assemble the T3SS sorting platform. We show that SpaOshort docks to the N-terminal region of SpaOLarge and integrates into the platform. Not essential,but critical for efficient effector secretion.Small protein,big role.

Interplay between SpaO variants shapes the architecture of the Salmonella type III secretion sorting platform | mBio Salmonella enterica is an increasing global public health threat. As part of its virulence arsenal, Salmonella relies on a type III secretion system (T3SS) or injectisome, a molecular injection device...

Happy to share our latest work!
Interplay between SpaO variants shapes the architecture of the Salmonella type III secretion sorting platform | mBio journals.asm.org/doi/full/10....

Host CD59 Potentiates the Type III Secretion System in Yersinia pseudotuberculosis

www.biorxiv.org/content/10.6...

Gene synteny and translational coupling of sctS and sctT facilitate assembly of the unique helical T3SS export apparatus in Salmonella Typhimurium
www.biorxiv.org/content/10.6...

I had the fun opportunity to write a Commentary for a JB paper by Bhattarai, Harshey et al. describing their Texas Two-Step model of cdG-mediated motility control.
Commentary: pubmed.ncbi.nlm.nih.gov/41705806/
Paper: journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology

Differential targeting of human pyroptotic caspase-5 and caspase-4 by Shigella OspC2 and OspC3 | mBio Shigella species are human-specific gram-negative pathogens that establish a replicative niche in intestinal epithelial cells by blocking pyroptosis, a key inflammatory cell death pathway. We reveal t...

Differential targeting of human pyroptotic caspase-5 and caspase-4 by Shigella OspC2 and OspC3 | mBio journals.asm.org/doi/10.1128/...

Substrate secretion by the different EHEC secretion systems during its interaction with epithelial cells Enterohemorrhagic Escherichia coli (EHEC) causes severe foodborne illness in humans. EHEC harbors five types of secretion systems (SSs) and unlike the type three secretion system (T3SS), the other ...

Full article: Substrate secretion by the different EHEC secretion systems during its interaction with epithelial cells www.tandfonline.com/doi/full/10....

A type III secretion system is required for Bordetella atropi invasion of host cells in vivo Author summary The free-living nematodes, C. elegans and its related species, are useful models to study host-pathogen interactions in whole animal settings since they share many similarities in immun...

A type III secretion system is required for Bordetella atropi invasion of host cells in vivo
dx.plos.org/10.1371/jour...

Congrats to the authors! This is pure gold for labs in the Global South, where every sequencing run counts

Salmonella relies on siderophore exploitation at low pH Abstract. Salmonella enterica, a prominent enteric pathogen, employs sophisticated iron acquisition mechanisms to overcome host-imposed iron limitation, no

What does competition for iron look like for pathogens in different environments? We had the chance to contribute to this great paper looking at Salmonella in the gut with the Cunrath Lab (UoStrasbourg). Salmonella relies on siderophore exploitation at low pH url: academic.oup.com/microlife/ar...

Oligomeric assembly of the gatekeeper InvE orchestrates hierarchical type III protein secretion in Salmonella Typhimurium | PNAS Type III secretion systems (T3SS) are critical virulence machines in many Gram-negative bacteria, enabling hierarchical secretion of translocases f...

📄 New paper out!
Please check out our latest publication, where we show how the gatekeeper protein InvE orchestrates hierarchical type III secretion in Salmonella Typhimurium via oligomeric assembly. #T3SS #DNAPAINT

www.pnas.org/doi/10.1073/...

amazing work! congrats!

Oligomeric assembly of the gatekeeper InvE orchestrates hierarchical type III protein secretion in Salmonella Typhimurium | PNAS Type III secretion systems (T3SS) are critical virulence machines in many Gram-negative bacteria, enabling hierarchical secretion of translocases f...

Oligomeric assembly of the gatekeeper InvE orchestrates hierarchical type III protein secretion in Salmonella Typhimurium | PNAS www.pnas.org/doi/10.1073/...

Bacteria break through one-micrometer-square passages by flagellar wrapping - Nature Communications Yoshioka et al. show that bacteria wrap their flagella to squeeze through near cell-width confinements, which allows symbiotic microbes to navigate constricted gut regions within insect hosts.

#NatMicroPicks

A bacterial symbiont wraps its flagllar filaments around its cell body to propel itself through narrow gut passages in its stinkbug host and hook flexibility is key for this 🪲🦠

by Yoshitomo Kikuchi & co @natcomms.nature.com

www.nature.com/articles/s41...

Effector–host interactome map links type III secretion systems in healthy gut microbiomes to immune modulation - Nature Microbiology Large-scale computational and in vitro analyses identify commensal type III secretion systems and substrates in the human gut microbiome that can interact with human proteins to modulate immune pathwa...

Effector–host interactome map links type III secretion systems in healthy gut microbiomes to immune modulation
www.nature.com/articles/s41...

Pseudomonas syringae histidine kinase BvgS acts as the sensory receptor of plant-derived putrescine to activate the type III secretion system and enhance bacterial virulence Plant diseases caused by Pseudomonas syringae pathovars pose a substantial threat to global crop production. While plants produce a range of secondary…

Pseudomonas syringae histidine kinase BvgS acts as the sensory receptor of plant-derived putrescine to activate the type III secretion system and enhance bacterial virulence

www.sciencedirect.com/science/arti...

Decoding the host–pathogen proteomic dialogue using surface labeling tools - Dubois - FEBS Letters - Wiley Online Library febs.onlinelibrary.wiley.com/doi/10.1002/...

Canine Providencia alcalifaciens: virulence factors and phylogenetic analysis of an emerging enteropathogen In 2019, an outbreak of acute haemorrhagic diarrhoea (AHD) in dogs in Norway was linked to Providencia alcalifaciens. A marked increase in diarrhoeal cases in dogs during the autumn, along with the fr...

Canine Providencia alcalifaciens: virulence factors and phylogenetic analysis of an emerging enteropathogen Open Access
www.microbiologyresearch.org/content/jour...

Mirdita Lab - Laboratory for Computational Biology & Molecular Machine Learning Mirdita Lab builds scalable bioinformatics methods.

My time in @martinsteinegger.bsky.social's group is ending, but I’m staying in Korea to build a lab at Sungkyunkwan University School of Medicine. If you or someone you know is interested in molecular machine learning and open-source bioinformatics, please reach out. I am hiring!
mirdita.org

Structural and Biophysical Characterization of the Yersinia Type Three Secretion System ATPase YscN Yersinia pestis was responsible for the Black Plague, one of the worst epidemiological disasters in recorded history. Today, Y. pestis, Y. enterocolitica, and Y. pseudotuberculosis remain clinically ...

PROTEINS: Structure, Function, and Bioinformatics | Protein Science Journal | Wiley Online Journal onlinelibrary.wiley.com/doi/10.1002/...

Bacterial reflect The gene moeA is involved structural colour of some bacterial colonies by modifying cell shape and colony organisation, thus causing a shift in reflected colour.

The gene moeA is involved in the structural colour of some bacterial colonies by modifying cell shape and colony organisation, thus causing a shift in reflected colour.

The import of biological research material is a silent barrier to biotechnology in the Global South - Nature Biotechnology Nature Biotechnology - The import of biological research material is a silent barrier to biotechnology in the Global South

The persistent challenge of importing biological research materials to the Global South at a reasonable cost, within acceptable timeframes, and with manageable regulatory complexity stalls progress before research and innovation can even begin go.nature.com/48ZIPHr
rdcu.be/eZond

Shigella OspF blocks rapid p38-dependent priming of the NAIP–NLRC4 inflammasome | PNAS www.pnas.org/doi/10.1073/...

A new mouse model of typhoid fever using Salmonella enterica serovar Paratyphi C as a surrogate pathogen | mBio The emergence of extensively drug-resistant Salmonella enterica serovar (S.) Typhi poses a serious threat to public health, but its host restriction to humans poses a challenge for studying pathogenes...

A new mouse model of typhoid fever using Salmonella enterica serovar Paratyphi C as a surrogate pathogen | mBio journals.asm.org/doi/10.1128/...

🔍Salmonella enterica contaminates essential food sources and causes serious illness. Peter Katsande takes us behind the scenes of their latest #MGEN publication investigating its genetic profile in the food supply chain of Zimbabwe. Read the full blog🔗 microb.io/3NtC4GA

Structural insights into the antibacterial function of the Pseudomonas putida effector Tke5
@pbernalt6ss.bsky.social, David Albesa-Jové and coworkers
link.springer.com/article/10.1...

JB Editor's Choice: Bhattarai, Harshey et al. propose a new model for YcgR::c-di-GMP regulation of flagellar motility in Salmonella/E. coli. They provide evidence for the first part of their model.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology

Hopefully this also opens the door to stronger science links between Europe and Latin America

Structural insights into the assembly and evolution of a complex bacterial flagellar motor
www.nature.com/articles/s41...

Host CD59 Potentiates the Type III Secretion System in Yersinia pseudotuberculosis Type III secretion systems (T3SS) play critical roles in disease for many Gram-negative pathogens. For Yersinia pseudotuberculosis ( Yptb ), the machinery delivers the Yop bacterial effectors into hos...

Host CD59 Potentiates the Type III Secretion System in Yersinia pseudotuberculosis
www.biorxiv.org/content/10.6...