@anilevetu
Assistant professor at Vrije University Amsterdam We study spatiotemporal control of gene expression. We develop tools to visualize and quantify single mRNAs in fungi and bacteria #RNA #single-molecule #imaging-analysis 🍄🦠🇮🇹➡️🇨🇭➡️🇺🇸➡️ 🇳🇱 www.tutuccilab.com
"we developed Multiplexed Error Robust Fluorescence In Situ Hybridization 2.0 (MERFISH 2.0), an optimized spatial transcriptomic imaging chemistry to enhance profiling of fragmented and highly crosslinked RNA"
www.biorxiv.org/content/10.6...
🔬🌿Official launch of the #AGILE_project, an MSCA doctoral network funded by the European Union. #AGILE aims to train a new generation of plant scientists.
Team CODED @igred.fr is looking for 15 international doctoral students. For more information and/or to apply :
👉 www.msca-agile.eu
You are finishing your PhD and looking to continue in science?
The Martin lab @biology-unige.bsky.social has an open postdoctoral position in cell biology to study cell-cell fusion. For more information, please consult mocel.unige.ch/research-gro....
Thanks for reposting!
Funded PhDs in Advanced AI for Multi-modal Spatial Biology at Queen Mary University of London
urldefense.com/v3/__https:/...
Mammalian cells form hibernating disomes akin to those in bacterial cells - but connected via ribosomal RNA
www.science.org/doi/10.1126/...
co-first with: @andschwarz.bsky.social
with: @lea-dietrich.bsky.social, @sgiando.bsky.social, @erin-schuman.bsky.social and many more
🧵 0/6
#MolBio 🧪
Ribosomal RNA expansion segments mediate the oligomerization of inactive animal ribosomes
www.science.org/doi/full/10....
From Erin Schuman's lab @erin-schuman.bsky.social
#RNAbiology
Out now in Cell Systems: We develop a technique to visualize influenza A virus in live cells with single viral RNA resolution. Combined with precise readouts of viral transcription, we map the whole influenza A viral life cycle and identify key bottlenecks in infection.
www.cell.com/cell-systems...
👉Excited to share our latest work @natmicrobiol.nature.com, revealing a decrease in cytoplasmic crowding during filamentous growth in the human fungal pathogen Candida albicans. Furthermore we found that inhibition of ribosome biogenesis can trigger filamentous growth in this pathogen
rdcu.be/eT1Su
Happy to announce that our review article on vesicle-coupled mRNA trafficking is now availalbe
@mibinet.bsky.social
#EMBOrnaLocalization
link.springer.com/article/10.1...
I’m happy to share the main result of my PhD, which you can find on bioRxiv www.biorxiv.org/content/10.6.... If you are interested in learning about a new way to perform DNA-PAINT multiplexing, which we call Combi-PAINT, or if you are interested in the study of mRNA conformation, keep reading! 1/10
Excited to share our new paper! We developed a method to visualize proteasomal degradation at the single–molecule level in live cells, enabling us to dissect distinct modes of substrate engagement, probe co-factor dependence, and study proteasome–ribosome collisions.
www.biorxiv.org/content/10.6...
Amazing!
A wisdom tooth...a painful problem you want to remove!
The World Champion of Fungus talks punk science in @nytimes.com. Thank you @alanburdick.bsky.social for sitting down with SPUN's @tobykiers.bsky.social, this year's @tylerprize.bsky.social Laureate.
🔗 Read here: buff.ly/4TVueaz
Happy New Year all. I know it's been a draining and difficult year for science in general, but wanted to share some good news: our intrabody paper has now been published in Science Advances with lots of additional data.
www.science.org/doi/10.1126/...
We need help keeping the world’s largest living library of mycorrhizal fungi alive. The INVAM mycorrhizal culture collection is at risk due to federal funding cuts. Its loss would be catastrophic.
Link to donate:
🔗 invam.ku.edu/donate
Read @theguardian.com article:
🔗 buff.ly/hkEGXgu
#RNAbiology #SpatialGeneExpression #RNAlocalization #Cellcycle #SpatialBio
Kudos to Anna Maekiniemi and Philipp Savakis for finishing this story that was long time in the making! This paper survived a change of country, the beginning of a lab, a pandemic, a maternity leave and much, much more!
10/10. Functional Consequences on Cell Cycle. ZIP code mutants exhibit increased G2/M fraction and larger birth size, supporting a model where CLB2 mRNA localization and translation help synchronize bud growth with mitotic entry.
9/ ZIP Code Rescue Restores Localization but Partially Protein Levels. Reintroducing the ZIP code into the 3′UTR rescues mRNA localization but only partially restores Clb2 protein expression, implying additional, not yet identifed, cis-elements influence translation.
8/ No Role for Known Translational Repressors. Unlike ASH1 mRNA, CLB2 translation is not regulated by Puf6, Khd1, or Ssd1, indicating distinct translational control mechanisms for different bud-localized mRNAs.
7/ Translation Efficiency Is Spatially Controlled. mRNA/protein co-localization analysis in single cells shows CLB2 mRNAs are more efficiently translated in the bud than in the mother cell, suggesting localized translation contributes to protein level regulation.
6/ Deletion of SHE2 or SHE3 significantly impairs CLB2 mRNA localization and reduces protein synthesis, confirming their role in the same pathway as the ZIP code.
5/ In wild-type cells, Clb2 protein levels correlate with bud growth, acting as a proxy for bud maturity. This correlation is lost in ZIP code mutants, uncoupling growth from mitotic progression.
4/ Loss of ZIP Code Disrupts Localization and Translation. Synonymous mutations destroying the ZIP code abolish bud localization, reduce Clb2 protein levels, and lead to prolonged budded phase and increased daughter cell size.
3/ Bud localization of CLB2 mRNA promotes its translation without altering the nuclear localization of Clb2 protein, indicating that mRNA localization is involved in spatial regulation of protein synthesis rather than protein localization, unlike what we know for most localized mRNAs.
2/ The ZIP Code is in the Coding Sequence. A structured RNA element (ZIP code) within the CLB2 coding sequence is necessary but not sufficient for mRNA localization and efficient protein synthesis.
1/ CLB2 mRNA Localizes to the Bud. During the G2/M phase of the yeast cell cycle, CLB2 mRNA is actively transported to the bud via the She2-She3 complex, while its protein product accumulates in the mother nucleus.
Happy to share that the our manuscript "Cyclin CLB2 mRNA localization and protein synthesis link cell cycle progression to bud growth" is now finally out in its final form @natcomms.nature.com
www.nature.com/articles/s41...
Below a short🧵 with the key findings!
