ChemSyBio - Berteau Lab - Univ Paris-Saclay, INRAE

Novel chemistry and structural perspectives in vitamin B12-dependent radical SAM enzymes Vitamin B12 is one of the most complex vitamins and an essential cofactor for humans, but also for bacteria, which have evolved different strategies for its biosynthesis and acquisition. For decades, ...

doi.org/10.1039/D5SC...

www.inrae.fr/dossiers/lau...

From labeled CO2 to CN! Our approach towards electrophilic cyanation is out in @jacs.acspublications.org! Great collaboration with Fabien Caillé! Congrats Maxime Alexandre and all co-authors! 🍾👏
pubs.acs.org/doi/full/10....
#isotope #C14 #C11 #cyanation
@agencerecherche.bsky.social @erc.europa.eu

Beyond Antibiotics: Emerging Strategies for Combating Bacterial Infection | Keystone Symposia Join us at the Keystone Symposia on Beyond Antibiotics: Emerging Strategies for Combating Bacterial Infection, May 2026, in Breckenridge, with field leaders!

Registration is now open for @keystonesymposia.bsky.social "Beyond #Antibiotics: Emerging Strategies Combating #BacterialInfection." Deadline for scholarships and abstract selected short talks is Jan 7! Join us this May for an amazing meeting! keysym.us/KSBeyondAntibiotics26 #KSBeyondAntibiotics26

Great collaboration with co-authors Maud Hofmann, Farouk Abdo, and @oborkowski.bsky.social.

⚡🌩️ From molecular to flow-cell: Our latest paper shows cobalt(III)triphenylcorrole for CO2 reduction to CO and methanol now out in ACS Catalysis.
In collaboration with ICMUB bourgogne

congratulations @afrza686.bsky.social @ajeetsinghm.bsky.social Bishnu and Marcello
@ipcm-sorbonne.bsky.social

The Radical S-Adenosyl-l-methionine Enzyme HydE Forms an Fe(I)Fe(I) Dimer En Route to the [FeFe] Hydrogenase H-Cluster [FeFe] hydrogenases are highly efficient metalloenzymes that catalyze hydrogen conversion via a sophisticated active site cofactor known as the H-cluster. Biosynthesis of its [2Fe]H subcluster, which ...

is the dimer core of the H-cluster of FeFe hydrogenase formed by rSAM chemistry in HydE?

pubs.acs.org/doi/full/10....

💥 Excited to introduce Bacformer 🦠 - the first foundation model for bacterial genomics. Bacformer represents genomes as sequences of ordered proteins, learning the “grammar” of how genes are arranged, interact and evolve.

Preprint 📝: biorxiv.org/content/10.1...

🧵 1/n

🦠🧍‍♀️From bacterial to human immunity.

We report in @science.org the discovery of a human homolog of SIR2 antiphage proteins that participates in the TLR pathway of animal innate immunity.
Co-led wt @enzopoirier.bsky.social by D. Bonhomme and @hugovaysset.bsky.social

www.science.org/doi/10.1126/...

We have written up a tutorial on how to run BindCraft, how to prepare your input PDB, how to select hotspots, and various other tips and tricks to get the most out of binder design!

github.com/martinpacesa...

🎶 Straight from the Lab to the Mic: when a Pasteur scientist turns her thesis into a rap 🎶
#FeteDeLaMusique

‘Remarkable’ new enzymes built by algorithm with physics know-how Nature - Computer approach creates synthetic enzymes 100 times more efficient than those designed by AI.

Computer algorithms have designed highly efficient synthetic enzymes from scratch

https://go.nature.com/43PmE5s

New on @biorxiv!!!!!!
We’re excited to share our latest paper on combining cell-free systems and machine learning to express full genome in vitro.

Check it out now! 👉 www.biorxiv.org/content/10.1...

Catch us at SEED in Houston this June to discuss this work.
#CellFree

Spectroscopic and Biochemical Characterization of the Noncanonical Radical SAM Enzyme ArsL, Involved in Arsinothricin Biosynthesis Radical SAM enzymes are the most widespread biocatalysts. These metalloenzymes, using S-adenosyl-l-methionine (SAM) and a [4Fe-4S] cluster as central cofactors, catalyze a broad range of chemically ch...

Radical SAM #enzyme & #antibiotic biosynthesis: ArsL, a novel [4Fe-4S] enzyme, cleaves the central S-adenosyl-L-methionine (SAM) cofactor to generate the 3-amino-3-carboxypropyl #radical instead of the 5′-deoxyadenosyl radical (5'-dA).

Amazing team work!

Check out:
pubs.acs.org/doi/10.1021/...

Our work on the synthesis of sceptrin, ageliferin, nigramide R, chabamide and others by photo(flow)catalysis are now on ChemRxiv. urlr.me/aJfu4r Congratulations to Mohammed Latrache & Amina Sesay @univparissaclay.bsky.social. Great collaboration with @arseniyadis.bsky.social @hiltonlab.bsky.social

Happy to represent bacterial immunity with great bacterial immunologist Tana Wein at a conference honouring the 40th birthday of Toll discovery, made in Drosophila.
Really fun to be celebrating the contribution of diverse organisms to our understanding of immunity 🪰🐘🦠.

Spectroscopic and Biochemical Characterization of the Noncanonical Radical SAM Enzyme ArsL, Involved in Arsinothricin Biosynthesis Radical SAM enzymes are the most widespread biocatalysts. These metalloenzymes, using S-adenosyl-l-methionine (SAM) and a [4Fe-4S] cluster as central cofactors, catalyze a broad range of chemically challenging transformations. The vast majority of radical SAM enzymes initiate their reaction by the homolytic cleavage of the SAM C5′–S bond and the generation of the central 5′-deoxyadenosyl radical (5′-dA·). In this study, by combining spectroscopic approaches with labeling and biochemical analyses, we show that ArsL, the key enzyme in the biosynthesis of the arsenic-containing antibiotic arsinothricin, catalyzes a unique reaction: the addition of the 3-amino-3-carboxypropyl radical (ACP·) to As(III). Remarkably, by exploiting several radical trapping strategies, we demonstrate that in sharp contrast to canonical radical SAM enzymes ArsL cleaves the SAM Cγ-S bond. In addition, using electron paramagnetic resonance (EPR) and hyperfine sublevel correlation (HYSCORE) spectroscopies, we establish that ArsL has a unique SAM binding mode, consistent with its catalytic properties and predicted structure. Notably, EPR and HYSCORE analyses support that SAM interacts with the radical SAM [4Fe-4S] cluster in an uncharacteristic conformation to form ACP·. Collectively, our study reveals that members of the superfamily of radical SAM enzymes are able to finely tune the binding of the SAM cofactor in order to perform unique chemistries.

Spectroscopic and Biochemical Characterization of the Noncanonical Radical SAM Enzyme ArsL, Involved in Arsinothricin Biosynthesis | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/...

💊 Researchers at Institut Curie, @cnrs.fr and @inserm.fr have developed a prototype drug that destroys refractory cancer cells by degrading their membranes.

🎥 Dr Raphaël Rodriguez tells us more on video ⤵️
ℹ️ Read the scientific publication in Nature: www.nature.com/articles/s41...

[Journal Club] Kinetic Redox Shotgun Proteomics Reveals Specific Lipopolysaccharide Effects on Intestinal Epithelial Cells, Mitigated by a Mn Superoxide Dismutase Mimic frenchbic.cnrs.fr/2025/05/11/k...

This is a wild paper. The anaerobic biochemistry is definitely worth your while (and beyond me to explain), but let me try to explain why this is also an evolutionary bombshell. This is about how nitrogen fixation evolved as we know it.

Evolutionary-scale prediction of atomic-level protein structure with a language model A protein language model enables structure prediction and analysis of more than 600 million metagenomic proteins.

Evolutionary-scale prediction of atomic-level protein structure with a language model | Science www.science.org/doi/10.1126/...

AI-guided Antibiotic Discovery Pipeline from Target Selection to Compound Identification Antibiotic resistance presents a growing global health crisis, demanding new therapeutic strategies that target novel bacterial mechanisms. Recent advances in protein structure prediction and machine ...

Excited to share our new preprint: AI-guided Antibiotic Discovery Pipeline from Target Selection to Compound Identification!
It includes a comprehensive benchmark of structure-based drug design (SBDD) methods and presents a full, practical pipeline for antibiotic discovery.
arxiv.org/abs/2504.11091

AI for research: the ultimate guide to choosing the right tool Curious about using artificial intelligence to boost your research? Here are the programs you shouldn’t miss.

Curious about using artificial intelligence to boost your research? Here are the programs you shouldn’t miss

https://go.nature.com/3RFlouC

Peroxiredoxins are some of the coolest enzymes out there—they don’t just protect cells from oxidative stress, they also form awesome donut-shaped structures! 🍩 Targeting them could lead to new antibiotics. #Microbiology #antibiotics #AMR #Antioxidants #BacterialPathogens

Exploring the principles behind antibiotics with limited resistance - Nature Communications This study shows that only those dual-targeting antibiotics limit resistance in Gram-negative pathogens that also target the membrane of the bacteria. This mechanism provides a basis for designing fut...

Exploring the principles behind antibiotics with limited resistance
www.nature.com/articles/s41...

SARS-CoV-2 infectivity can be modulated through bacterial grooming of the glycocalyx | mBio Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for coronavirus disease 2019, can infect the gastrointestinal (GI) tract, and individuals who exhibit GI symptoms of...

SARS-CoV-2 infectivity can be modulated through bacterial grooming of the glycocalyx | mBio journals.asm.org/doi/10.1128/...

💬 Huge thanks to all who contributed! Shoutout to those I know are on this app: @bkell1123.bsky.social @smallard.bsky.social @gilbertjacka.bsky.social @chemsybio.bsky.social

🔥 Finally published in mBio @asm.org! An update from the 2020 pre-print: “SARS-CoV-2 infectivity can be modulated through bacterial grooming of the glycocalyx.” 🔗https://journals.asm.org/doi/10.1128/mbio.04015-24 🧵👇

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