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www.protocols.io

@protocolsio

A secure platform for developing and sharing reproducible methods.

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21.09.2023
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Latest posts by www.protocols.io @protocolsio

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Last month, we highlighted Protocols.io as a new resource available at @bodleian.ox.ac.uk. Next week, as part OxFOS, you can sign up and join the Springer Nature team for a training session introducing the platform to researchers either in person here at the RSL or online

go.glam.ox.ac.uk/proto

26.02.2026 11:00 ๐Ÿ‘ 3 ๐Ÿ” 2 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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Started this project years ago, but now everything is deposited on @addgene.bsky.social and a short @protocolsio.bsky.social is online. Try it out to make your own (cheap) homemade nuclease/Benzonase!

dx.doi.org/10.17504/protocols.io.261ged7xov47/v2

Thank you @jcoker10.bsky.social and Michael Lim!

20.02.2026 10:31 ๐Ÿ‘ 28 ๐Ÿ” 10 ๐Ÿ’ฌ 2 ๐Ÿ“Œ 2
Francis Crick Institute The Francis Crick Institute (formerly the UK Centre for Medical Research and Innovation) is a biomedical research centre in London, which was established in 2010 and opened in 2...

We've updated our protocols.io page to highlight a couple of the protocols published by Crick authors recently.

If you're at the Crick, don't forget you can get your flat pdfs converted to the protocols.io format at no cost! ๐Ÿ˜€

www.protocols.io/organization...

17.02.2026 12:04 ๐Ÿ‘ 3 ๐Ÿ” 3 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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Labeling of nascent RNA in the C. elegans intestine Transcriptional regulation in C. elegans has been difficult to study at the level of nascent RNA because nucleotide analogs do not readily penetrate the cuticle. Here, we establish an ex vivoโ€ฆ

A Lab Protocol by Omid Gholamalamdari and Stephanie C. Weber details an optimized method for labeling of nascent RNA transcripts in the C. elegans intestine, enabling spatial analysis of transcription dynamics.

plos.io/3Ox4rnO

@protocolsio.bsky.social link: plos.io/3O9Jv6g

10.02.2026 15:00 ๐Ÿ‘ 5 ๐Ÿ” 4 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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Generation of Stickleback intestinal organoid Intestinal organoids offer significant advantages over conventional 2D cultures, providing a physiologically relevant 3D architecture that enables more realistic disease modelin...

New @protocolsio.bsky.social post describing our new protocol for generating intestinal organoids for #stickleback: www.protocols.io/view/generat...
With work by postdoc Arshad Padhiar, funded by @moorefound.bsky.social

03.02.2026 14:32 ๐Ÿ‘ 5 ๐Ÿ” 2 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0

Given the recent #Nipah outbreak in India, here are two #Nanopore protocols for NiV genomic sequencing in support of rapid outbreak response:

IPC: www.protocols.io/view/sequenc...
icddr,b/CDC: journals.asm.org/doi/10.1128/...

03.02.2026 08:00 ๐Ÿ‘ 2 ๐Ÿ” 2 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
Promotional graphic for Springer Nature and protocols.io featuring an event announcement. The left side has a light blue background with the Springer Nature logo at the top and text reading: โ€œJoin experts for interactive sessions. Jan 21: protocols.io Hackathon. Jan 22: Methods Mythbusting Panel.โ€ A dark blue button below says โ€œRegister today.โ€ The right side shows the protocols.io logo and a large text design that says โ€œI โ™ฅ Methodsโ€ with a red heart symbol, followed by โ€œLove Methods Weekโ€ and the hashtag #LoveMethods26.

Promotional graphic for Springer Nature and protocols.io featuring an event announcement. The left side has a light blue background with the Springer Nature logo at the top and text reading: โ€œJoin experts for interactive sessions. Jan 21: protocols.io Hackathon. Jan 22: Methods Mythbusting Panel.โ€ A dark blue button below says โ€œRegister today.โ€ The right side shows the protocols.io logo and a large text design that says โ€œI โ™ฅ Methodsโ€ with a red heart symbol, followed by โ€œLove Methods Weekโ€ and the hashtag #LoveMethods26.

๐Ÿ’ก Did you know? Methods are among the most cited research outputs.
For #LoveMethods26, join experts for interactive sessions:
๐Ÿ‘‰ Jan 21: @protocolsio.bsky.social Hackathon
๐Ÿ‘‰ Jan 22: Methods Mythbusting Panel
spklr.io/633288eAVM

#OpenScience

15.01.2026 14:21 ๐Ÿ‘ 6 ๐Ÿ” 4 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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We will import and format your protocol Paper protocols floating on your benchtops? We can digitize them for you!

Happy International Love Methods week!

The Crick partners with @protocolsio.bsky.social to share open methods, and if you happen to be a Crick researcher, you can use their protocol writing up service for free (while codes last!)

www.protocols.io/we-enter-pro...

19.01.2026 09:21 ๐Ÿ‘ 6 ๐Ÿ” 3 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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Join protocols.io and Springer Nature editors at Love Methods Week 2026 Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth...

Join Springer Nature editors and the protocols.io team for two exciting events as part of #LoveMethods26!

๐Ÿ’ปTransform your protocols at our Hackathon on Jan 21

๐Ÿ”ฎGain insight on methods publishing at our Methods Mythbusting on Jan 22

๐Ÿ‘‰FREE registration & more details:

09.01.2026 18:00 ๐Ÿ‘ 7 ๐Ÿ” 5 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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Join protocols.io and Springer Nature editors at Love Methods Week 2026 Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth...

Join Springer Nature editors and the protocols.io team for two exciting events as part of #LoveMethods26!

๐Ÿ’ปTransform your protocols at our Hackathon on Jan 21

๐Ÿ”ฎGain insight on methods publishing at our Methods Mythbusting on Jan 22

๐Ÿ‘‰FREE registration & more details:

09.01.2026 18:00 ๐Ÿ‘ 7 ๐Ÿ” 5 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
Graphic representing our monthly New Resource promotion featuring the name of the Library and this months chosen product - Protocols.io

Graphic representing our monthly New Resource promotion featuring the name of the Library and this months chosen product - Protocols.io

Our promoted new resource for January is Protocols.io. Using Protocols.io researchers can create and share detailed experimental protocols with colleagues or the wider scientific community

www.protocols.io

07.01.2026 14:02 ๐Ÿ‘ 3 ๐Ÿ” 1 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
Methods Mythbusting: Insights from Journal Editors and protocols.io This webinar will be held at: 3pm CET / 2pm GMT / 9am EST / 6am PST Join us for a lively session where we tackle common misconceptions about protocols and methods publishing. H...

Join us for โ€œMethods Mythbusting: Insights from Journal Editors and protocols.ioโ€protocols.io/webinars/met...rs

at Love Methods Week 2026excelscior.uc.pt/love-methods...6/

#LoveMethods26

15.12.2025 08:18 ๐Ÿ‘ 4 ๐Ÿ” 2 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0

๐ŸŽ‰ We are thrilled to announce that our protocol โ€œLibrary construction for ONT long read cDNAโ€, developed at our GenomiqueENS core facility, is available on protocol.io. dx.doi.org/10.17504/protocols.io.4r3l21oepg1y/v1

10.12.2025 14:25 ๐Ÿ‘ 1 ๐Ÿ” 2 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 0

๐ŸงฌSearching for a Nanopore RNA-seq protocol that generates longer reads than the manufacturerโ€™s protocol, and applicable for low-input material ? ๐Ÿค”

10.12.2025 14:25 ๐Ÿ‘ 3 ๐Ÿ” 2 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 0
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Really liking the idea and output of GAQETv2 - such a cool tool for structural genome annotation QC!!
www.protocols.io/view/structu...

26.11.2025 10:42 ๐Ÿ‘ 2 ๐Ÿ” 1 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
New collaboration empowers thousands of researchers to share research methods, improve reproducibility and drive global scientific progress | Springer Nature Group | Springer Nature Heidelberg | London, 27 November 2025 Springer Nature and Research4Life extend partnership to include access to protocols.io for scientists in over

Exciting news: Premium protocols.io is now available via Research4Life to researchers in low- & middle-income countriesโ€”at no or low cost.

๐ŸŒŸ Full Springer Nature press release: group.springernature.com/gp/group/med...

#OpenScience #Reproducibility #GlobalCollaboration #Research4Life #protocolsio

27.11.2025 17:16 ๐Ÿ‘ 3 ๐Ÿ” 2 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
The image promotes a new collaboration empowering researchers to share methods, improve reproducibility, and drive global scientific progress. It includes a close-up of a computer chip with a brain illustration and the Springer Nature logo.

The image promotes a new collaboration empowering researchers to share methods, improve reproducibility, and drive global scientific progress. It includes a close-up of a computer chip with a brain illustration and the Springer Nature logo.

Springer Nature has extended its partnership with @r4lpartnership.bsky.social to provide researchers in more than 90 low-middle-income countries with no-cost or low-fee access to premium features on @protocolsio.bsky.social.

Learn more: group.springernature.com/gp/group/med...

#Research ๐Ÿงช

27.11.2025 16:06 ๐Ÿ‘ 2 ๐Ÿ” 2 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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The Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub) ...

Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub librehub.github.io) is an amazing initiative from @makertobey.bsky.social and colleagues!

18.11.2025 20:30 ๐Ÿ‘ 3 ๐Ÿ” 3 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 0
GitHub - victorgcb1987/GAQET2 Contribute to victorgcb1987/GAQET2 development by creating an account on GitHub.

We have done our first @protocolsio.bsky.social. Are you interested about #genome_annotation quality evaluation? We have developed a tool called #GAQET2 (github.com/victorgcb198...) which runs several tools like #AGAT, #BUSCO, and many other tools. You can find it at www.protocols.io/view/structu...

18.11.2025 14:52 ๐Ÿ‘ 12 ๐Ÿ” 6 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 0
Construyendo protocolos cientรญficos reproducibles y abiertos con Protocols.io
Construyendo protocolos cientรญficos reproducibles y abiertos con Protocols.io YouTube video by LIBRE hub

It was great to hear from @lteytelman.bsky.social about Protocols.io today for our LIBRE hub channel on open science! We already have a tutorial in Spanish: youtu.be/RuBKWouEyL8?...

18.11.2025 20:59 ๐Ÿ‘ 3 ๐Ÿ” 2 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0

With the full CRISPR 7-11 protocol here :)

11.11.2025 17:51 ๐Ÿ‘ 10 ๐Ÿ” 5 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
Graphic with a dark teal background featuring three illustrated circular icons representing different research teams.

On the left, Team Kordower icon shows colorful antibody shapes labeled โ€œNeuro-Immune Interactions.โ€

In the center, Team Liddle icon depicts a stylized brain with interconnected circuitry labeled โ€œCircuitry and Brain-Body Interactions.โ€

On the right, Team Rio icon shows a human head outline with a DNA strand inside, labeled โ€œPD Functional Genomics.โ€

A thin gradient line runs along the bottom, shifting from purple to blue to green, with the ASAP logo in the lower right corner.

Graphic with a dark teal background featuring three illustrated circular icons representing different research teams. On the left, Team Kordower icon shows colorful antibody shapes labeled โ€œNeuro-Immune Interactions.โ€ In the center, Team Liddle icon depicts a stylized brain with interconnected circuitry labeled โ€œCircuitry and Brain-Body Interactions.โ€ On the right, Team Rio icon shows a human head outline with a DNA strand inside, labeled โ€œPD Functional Genomics.โ€ A thin gradient line runs along the bottom, shifting from purple to blue to green, with the ASAP logo in the lower right corner.

๐Ÿ‘ This monthโ€™s Network Spotlight goes to Teams Kordower, Liddle, & Rio!

Their open-access protocols ranked among the top 100 most viewed on @protocolsio.bsky.social in August ๐ŸŽ‰

โžก๏ธ Mouse Perfusions (bit.ly/4qH6rIY)
โžก๏ธ Y-Maze (bit.ly/47aITTC)
โžก๏ธ Culture/transfection of HEK293T cells (bit.ly/4o4ytfR)

31.10.2025 15:05 ๐Ÿ‘ 1 ๐Ÿ” 1 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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Optimisation of whole cell human depletion provides increased sensitivity and microbial genome coverage from respiratory metagenomic assays Metagenomics is being adopted worldwide as a laboratory developed test. We have previously reported a unified metagenomic method for the direct detection of microorganisms from respiratory samples, de...

Our new preprint covering the improvements we made to our respiratory metagenomic assay is out now:

www.medrxiv.org/content/10.1...

โœ… Now implemented in 6 NHS hospitals
โœ… Increased sensitivity
โœ… Increased pathogen genome coverage

(1/3)

20.10.2025 10:35 ๐Ÿ‘ 7 ๐Ÿ” 5 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 0
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โ€˜REF needs dedicated section for research methodsโ€™ - Research Professional News Hidden REF Festival: Conference hears plea to fill gap in Research Excellence Framework

Hereโ€™s a write-up that includes my lightning talk and reflections on why technicians must be recognised in the REF:
๐Ÿ”— www.researchprofessionalnews.com/rr-news-uk-r...
Hope to work with others to get methods formally recognised as reportable research outputs in a category of their own.

09.10.2025 10:40 ๐Ÿ‘ 5 ๐Ÿ” 2 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 0
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The Spatial Thinking Ability Test (STAT-24):Classroom administration, scoring, and data management This protocol standardizes the classroom administration, scoring, and data management of the Spatial Thinking Ability Test (STAT-24), a category-balanced instrument for secondar...

3. The Spatial Thinking Ability Test (STAT-24):Classroom administration, scoring, and data management
www.protocols.io/view/the-spa...

06.10.2025 18:31 ๐Ÿ‘ 2 ๐Ÿ” 0 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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Water and sediment sampling in the sea to discover marine bacteria This protocol aims to show the strategy for obtaining water and sediment samples on board an oceanographic vessel at sea. The samples obtained can be used to identify marine bac...

2. Water and sediment sampling in the sea to discover marine bacteria
www.protocols.io/view/water-a...

06.10.2025 18:31 ๐Ÿ‘ 2 ๐Ÿ” 0 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 0
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Intracellular Metabolite Extraction, Peak Detection with El-MAVEN and Quantification Using Python This protocol outlines a comprehensive workflow for intracellular metabolite analysis from microbial cultures. It begins with the rapid quenching and extraction of metabolites u...

1. Intracellular Metabolite Extraction, Peak Detection with El-MAVEN and Quantification Using Python V.2
www.protocols.io/view/intrace...

06.10.2025 18:31 ๐Ÿ‘ 1 ๐Ÿ” 0 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 0
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Bring structure to your research - protocols.io A secure platform for developing and sharing reproducible methods.

Explore our new featured protocols protocols.io/welcome

06.10.2025 18:31 ๐Ÿ‘ 4 ๐Ÿ” 1 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 0
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Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io Research is a global endeavour, yet the expectation to communicate in English often creates barriers and an additional burden for non-native speakers. At the lab bench or in t...

I've been dreaming about this for a decade and it's now live!

"Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io"
www.protocols.io/blog/global-...

02.10.2025 18:45 ๐Ÿ‘ 5 ๐Ÿ” 1 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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Plate CUT&Tag (Meers Lab version) This protocol describes a method for carrying out CUT&Tag-Direct in 96 well microplates for high-throughput processing of samples by a single operator with only standard lab...

We published a detailed protocol for our Plate-CUT&Tag method on @protocolsio.bsky.social to accompany our recent preprint (www.biorxiv.org/content/10.1...) give it a try! Feedback welcome! dx.doi.org/10.17504/protocols.io.n2bvjed5wgk5/v1

22.09.2025 17:45 ๐Ÿ‘ 29 ๐Ÿ” 13 ๐Ÿ’ฌ 2 ๐Ÿ“Œ 0