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Federico Gasparoli

@fedegasparoli

๐Ÿ”ฌ๐Ÿง‘โ€๐Ÿ’ป Research Associate - Image Analysis Colaboratory @ Harvard Medical School

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07.12.2023
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Latest posts by Federico Gasparoli @fedegasparoli

We opened 4 PhD positions at @humantechnopole.bsky.social together with Polimi (see ๐Ÿ‘‡).

My lab offers a PhD project on "Computational Morphogenesis": use state-of-the-art machine learning methods together with realistic simulations to build digital twins of developing tissue.

Apply by March 26.

05.03.2026 07:46 ๐Ÿ‘ 20 ๐Ÿ” 12 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 0

PAPER OUT โœจ How can we make smart microscopy more interoperable? What are the technical and cultural challenges? 30+ people from academia and industry propose a roadmap: doi.org/10.1515/mim-... Also a review of applications and repo of implementations. Join the discussion! smartmicroscopy.github.io

03.03.2026 17:32 ๐Ÿ‘ 45 ๐Ÿ” 17 ๐Ÿ’ฌ 2 ๐Ÿ“Œ 1
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Apply to join #BoBiAC2026 this summer! You'll learn how to use Python to segment fluorescence images w/tools like @ilastik-team.bsky.social & CellposeSAM, & even learn how to measure cell features, neighborhood relationships, & colocalization. No coding experience needed! More info: bobiac.github.io

03.03.2026 21:16 ๐Ÿ‘ 6 ๐Ÿ” 3 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0

Apply to join us this July for a crash course on Python & bioimage analysis tailored specifically for beginners! Applications due May 18th ๐Ÿ”ฌ๐Ÿ’ป #BoBiAC2026 bobiac.github.io

03.02.2026 21:42 ๐Ÿ‘ 9 ๐Ÿ” 5 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
Preview
GitHub - imaging-formats/bffile: Modern Bio-Formats wrapper with clean lazy Python API Modern Bio-Formats wrapper with clean lazy Python API - imaging-formats/bffile

Rewrote my Python bioformats wrapper (from aicsimageio/bioio) as a standalone package:

- fully bootstrapped Java setup (just pip install)
- lazy, repeatably-indexable Array object
- fully spec-compliant OME-Zarr group obj.
- xarray/dask exports

github.com/imaging-form...
v0.0.rc1 on PyPI

16.02.2026 21:06 ๐Ÿ‘ 60 ๐Ÿ” 23 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 1

Organized by @hms-iac.bsky.social & @citehms.bsky.social at Harvard Medical School and sponsored by BioImaging North America @bioimagingna.bsky.social.

03.02.2026 21:36 ๐Ÿ‘ 3 ๐Ÿ” 0 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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๐Ÿ“ฃย We are accepting applications for the 2026 Boston Bioimage Analysis Course (BoBiAC): bobiac.github.io! Join us this July at Harvard Medical School for aย 6-day intensive hands-on courseย to learn bioimage analysis with Python!
Apply by May 18th! No prior Python experience required!
๐Ÿงซ->๐Ÿ”ฌ->๐Ÿ’ป->๐Ÿ“Š

03.02.2026 21:36 ๐Ÿ‘ 24 ๐Ÿ” 17 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 2

Applications for QI 2026 are due on Friday! ๐Ÿ”ฌ ๐Ÿ–ฅ๏ธ ๐Ÿ“Š ๐Ÿ˜บ

28.01.2026 18:45 ๐Ÿ‘ 3 ๐Ÿ” 3 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0

Don't let the price of QI deter you!
QI offers financial aid through NCI, HHMS, and @bioimagingna.bsky.social.
Include a brief statement of need with your application.
Aid is distributed by CSHL (not the course instructors).
Many of our students - sometimes all! - receive significant support.

28.01.2026 18:57 ๐Ÿ‘ 3 ๐Ÿ” 5 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0

๐Ÿ”ฌ ๐Ÿ–ฅ๏ธ ๐Ÿ“Š!

28.01.2026 19:44 ๐Ÿ‘ 2 ๐Ÿ” 1 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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QI Class of 2025! Centroid = @sciencedoodles.bsky.social & @florianjug.bsky.social / first minima = @talley.codes & me.

Applications for 2026 due on Friday!

meetings.cshl.edu/courses.aspx...

28.01.2026 19:43 ๐Ÿ‘ 16 ๐Ÿ” 10 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 1

Join us at CSHL for Quantitative Imaging: From Acquisition to Analysisโ€”two weeks of advanced imaging, analysis, and hands-on labs with cutting-edge microscopes and open source software!
๐Ÿ“… April 6โ€“21, 2026
๐Ÿ“ Applications due Friday Jan 30, 2026

27.01.2026 16:39 ๐Ÿ‘ 12 ๐Ÿ” 7 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 3

๐Ÿšจ Only a few days left to apply for maybe the best microscopy course there is!!! ๐Ÿšจ
๐Ÿ‘‡๐Ÿ‘‡๐Ÿ‘‡๐Ÿ‘‡๐Ÿ‘‡๐Ÿ‘‡๐Ÿ‘‡๐Ÿ‘‡๐Ÿ‘‡๐Ÿ‘‡๐Ÿ‘‡

21.01.2026 22:37 ๐Ÿ‘ 21 ๐Ÿ” 15 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
Preview
Quantitative Imaging: From Acquisition to Analysis Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.

Re-upping: if you want to learn microscopy and image analysis - REALLY learn them, in an intense and hands on way - this is a really great place to do it. Applications close Jan 30

meetings.cshl.edu/courses.aspx...

22.01.2026 12:23 ๐Ÿ‘ 4 ๐Ÿ” 3 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0

๐Ÿ”ฌ๐ŸšจNew preprint alert! ๐Ÿšจ๐Ÿ”ฌ

We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology

www.biorxiv.org/content/10.6...

#SuperResolution #CellBiology

20.01.2026 16:12 ๐Ÿ‘ 44 ๐Ÿ” 18 ๐Ÿ’ฌ 5 ๐Ÿ“Œ 1

Want to up your microscopy and image analysis game?

Applications open now for QI 2026! Join us in cold spring harbor for two weeks of intensive training in all things microscopy.

09.01.2026 13:28 ๐Ÿ‘ 7 ๐Ÿ” 3 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
Preview
Quantitative Imaging: From Acquisition to Analysis Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.

๐Ÿ”ฌ ๐Ÿ–ฅ๏ธ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6โ€“21, 2026)!

An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools.

๐Ÿ—“๏ธ Apply online by Jan 30, 2026

06.01.2026 19:18 ๐Ÿ‘ 44 ๐Ÿ” 32 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 2
A fluorescent embryo on black text, with the words 
Halfway to I2K: Virtual Tutorials on Image Analysis
November 17-19, 2025
Virtual Conference for beginners to developers
i2kconference.org
Image Credit - "Sweet Embryo",Travis D. Carney, BINA Image Contest 2024

A fluorescent embryo on black text, with the words Halfway to I2K: Virtual Tutorials on Image Analysis November 17-19, 2025 Virtual Conference for beginners to developers i2kconference.org Image Credit - "Sweet Embryo",Travis D. Carney, BINA Image Contest 2024

Just 4 days until the start of I2K and its 33 totally free image analysis tutorials and events! Please share with your "home networks", especially early career researchers - the videos will be amazing and high-impact no matter how many people attend live, BUT (1/x)

13.11.2025 14:32 ๐Ÿ‘ 52 ๐Ÿ” 48 ๐Ÿ’ฌ 1 ๐Ÿ“Œ 0
Figure legend: Categories and capabilities of smart microscopy systems integrating real-time image analysis and feedback control. Top: Smart microscopy workflows can be classified based on the driving logic behind decision-making: Event-driven (reacting to rare biological events), Outcome-driven (using feedback-control to steer biological systems toward a desired state), Quality-driven (optimizing signal quality or imaging metrics), and Information-driven (guided by models that predict which measurements/perturbations will yield the most informative data). Middle: Central feedback loop between the microscope and an image analysis system, which continuously
exchanges images and commands to guide acquisition dynamically. Bottom: Key control actions enabled by smart microscopy: adjusting imaging modality (e.g. switching from brightfield to fluorescence, adjusting sampling rate), repositioning the field of view (e.g. tracking, drift correction), optimizing acquisition settings (e.g. adaptive optics),
and performing photomanipulation (e.g. FRAP, ablation, optogenetics).

Figure legend: Categories and capabilities of smart microscopy systems integrating real-time image analysis and feedback control. Top: Smart microscopy workflows can be classified based on the driving logic behind decision-making: Event-driven (reacting to rare biological events), Outcome-driven (using feedback-control to steer biological systems toward a desired state), Quality-driven (optimizing signal quality or imaging metrics), and Information-driven (guided by models that predict which measurements/perturbations will yield the most informative data). Middle: Central feedback loop between the microscope and an image analysis system, which continuously exchanges images and commands to guide acquisition dynamically. Bottom: Key control actions enabled by smart microscopy: adjusting imaging modality (e.g. switching from brightfield to fluorescence, adjusting sampling rate), repositioning the field of view (e.g. tracking, drift correction), optimizing acquisition settings (e.g. adaptive optics), and performing photomanipulation (e.g. FRAP, ablation, optogenetics).

๐Ÿ”ฌ๐Ÿง  Our paper on smart microscopy & the issue of interoperability! www.biorxiv.org/content/10.1... LONG THREAD WARNING: Smart microscopy uses real-time image analysis to automatically guide the acquisition or perturbation of the sample (closed feedback-control loop). Many applications exist:

21.08.2025 14:21 ๐Ÿ‘ 35 ๐Ÿ” 22 ๐Ÿ’ฌ 2 ๐Ÿ“Œ 1
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Check out our amazing #bobiac2025 cohort! Was a joy to teach this group! Thanks @bioimagingna.bsky.social for generously supporting this 6 week bioimage analysis w/Python course!

29.07.2025 18:20 ๐Ÿ‘ 6 ๐Ÿ” 3 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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Day 1 of #bobiac2025 ๐Ÿ

The day started strong with @talley.codes speaking about the Python ecosystem and uv. Students then gave short talks about their research (#coolscience). It was then time to code in Python with @sciencedoodles.bsky.social!

14.07.2025 20:27 ๐Ÿ‘ 7 ๐Ÿ” 4 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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โ€ผ๏ธ3 days left to apply to the Boston Bioimage Analysis Course (BoBiAC)! ๐Ÿ’ป

Get started w/Python & learn to analyze fluorescence microscopy images โ€” no programming experience required!

Fee waivers available!

๐Ÿ—“๏ธ Apply by June 1st!
๐ŸŒ iac.hms.harvard.edu/bobiac/2025/

30.05.2025 15:16 ๐Ÿ‘ 4 ๐Ÿ” 4 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
custom github css

custom github css

for you other colorblind coders out there, tired of missing the red X in your github actions logs

.color-fg-danger, .fgColor-danger {color: magenta !important;}
.octicon-x {color: magenta !important;}

you're welcome

21.05.2025 18:31 ๐Ÿ‘ 20 ๐Ÿ” 6 ๐Ÿ’ฌ 4 ๐Ÿ“Œ 0
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๐Ÿ’ปย Apply to our beginner-friendly Boston Bioimage Analysis Course!

Get started with Python and learn to analyze fluorescence microscopy images โ€” no coding experience required!

Fee waivers available thanks to funding from @bioimagingna.bsky.social!

๐Ÿ—“๏ธ Apply June 1st!
iac.hms.harvard.edu/bobiac/2025/

08.05.2025 14:39 ๐Ÿ‘ 18 ๐Ÿ” 9 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 2
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We are proudly hosting

๐ƒ๐ซ. ๐“๐š๐ฅ๐ฅ๐ž๐ฒ ๐‹๐š๐ฆ๐›๐ž๐ซ๐ญ @talley.codes (CITE, Harvard Medical School):

๐’๐ข๐ฆ๐ฎ๐ฅ๐š๐ญ๐ข๐ง๐  ๐ซ๐ž๐š๐ฅ๐ข๐ฌ๐ญ๐ข๐œ ๐ฅ๐ข๐ ๐ก๐ญ ๐ฆ๐ข๐œ๐ซ๐จ๐ฌ๐œ๐จ๐ฉ๐ฒ ๐ข๐ฆ๐š๐ ๐ž๐ฌ ๐ฎ๐ฌ๐ข๐ง๐  ๐ฆ๐ข๐œ๐ซ๐จ๐ฌ๐ข๐ฆ

When: ๐Œ๐š๐ฒ ๐Ÿ๐Ÿ—๐ญ๐ก @ ๐Ÿ๐Ÿ ๐š๐ฆ ๐„๐ƒ๐“ (Boston time)

Join us on Zoom! harvard.zoom.us/j/9762343464...

07.05.2025 15:58 ๐Ÿ‘ 40 ๐Ÿ” 18 ๐Ÿ’ฌ 2 ๐Ÿ“Œ 3
Ad for the Bioimage Analysis Beginnings Bootcamp at the Broad Institute. 

Where & When
Broad Institute, 415 Main Street, Cambridge MA (in-person only)
Mondayโ€“Friday, August 4โ€“8, 20259:00 AM โ€“ 5:00 PM

About the Bootcamp
Learn core bioimage analysis skills using open-source tools for light
microscopy in this hands-on, in-person course. The Bootcamp
covers annotation, segmentation, and deep learning fundamentals.

Fee: $600 (Academic/Non-Profit) | $1000 (Industry/For-Profit)
For more information email ImagingAdmin@broadinstitute.org

Ad for the Bioimage Analysis Beginnings Bootcamp at the Broad Institute. Where & When Broad Institute, 415 Main Street, Cambridge MA (in-person only) Mondayโ€“Friday, August 4โ€“8, 20259:00 AM โ€“ 5:00 PM About the Bootcamp Learn core bioimage analysis skills using open-source tools for light microscopy in this hands-on, in-person course. The Bootcamp covers annotation, segmentation, and deep learning fundamentals. Fee: $600 (Academic/Non-Profit) | $1000 (Industry/For-Profit) For more information email ImagingAdmin@broadinstitute.org

BIG NEWS - for the first time ever, we're running a Bioimage Analysis Beginnings Bootcamp. Learn #bioimageanalysis from awesome folks, and gain familiarity with some new tools as well as how to pick the right tool in the first place. Aug 4th-8th - apply here (soon!) forms.gle/d2fCXsrWHVP8...

01.05.2025 21:22 ๐Ÿ‘ 63 ๐Ÿ” 41 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 4

New to bioimage analysis & Python? Want to quantify fluorescence microscopy images, explore segmentation & classification, & try colocalization analysis using Python?

If so, this course is for you! Apply by June 1st! ๐Ÿ”ฌ๐Ÿ’ปhttps://iac.hms.harvard.edu/bobiac/2025

22.04.2025 15:10 ๐Ÿ‘ 6 ๐Ÿ” 3 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0

Can highly recommend! Not only is Python one of the most versatile tools in bioimage analysis, but bioimage analysis itself is also an excellent way for visual learners to learn and improve their Python skills! And it is led by fantastic people!

22.04.2025 15:32 ๐Ÿ‘ 3 ๐Ÿ” 2 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0
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โ€ผBREAKINGโ€ผ
Weโ€™ve secured new funding for the Boston Bioimage Analysis Course (BoBiAC) this July at Harvard Med School! Course fees down 37%, with fee waivers for academic applicants, for an intensive hands-on intro to bioimage analysis with Python!

Apply by June 1st!
iac.hms.harvard.edu/bobiac/2025

22.04.2025 14:55 ๐Ÿ‘ 19 ๐Ÿ” 12 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 2
BoBiAC logo incorporating a laptop
Boston Bioimage Analysis Course | 2025

BoBiAC logo incorporating a laptop Boston Bioimage Analysis Course | 2025

Are you looking to boost your bioimage analysis skills? If so, check out the Boston Bioimage Analysis Course run by @hms-iac.bsky.social and @citehms.bsky.social!
Apply by 16 May 2025
focalplane.biologists.com/2025/04/10/b...

17.04.2025 15:37 ๐Ÿ‘ 8 ๐Ÿ” 5 ๐Ÿ’ฌ 0 ๐Ÿ“Œ 0