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08.01.2026 16:42
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Ah, interesting! If the amplicon data comes from a complex sample, the different ASV proportions could result from non-isolated strains in the microbiome sample, sharing one V4 copy, but not the other and therefore skewing the V4-copy-ratios? then, the least relabund ASV would be the most realistic?
Once, I just used vmatchPattern to find the ASV fasta in the isolate fasta. Theoretically, different 16S sequences of the same bug should yield different ASVs. Or do I not get the problem?
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Hey @enirenberg.bsky.social, my favorite online immunologist!
Do you have an opinion on the immunological surrogates like the Il-10/Il-17 ratio in studies like this. share.google/CvnewT1FoN6D...
I mean apart from the small effect size, do we know this corresponds to meaningful endpoints?