Douglas W. Houston

[1/6] Two new preprints out today from the OGA team. We've quantified the ethical costs of poor antibody validation for the first time — and developed a consensus plan to fix it. Thread 🧵

#Dros26 Day 3

Did you know DSHB houses over 1600 Drosophila antibodies?

Browse the collection and more: dshb.biology.uiowa.edu

📸 Drosophila germarium labeled with DSHB anti-Vasa (green) and anti-hts (magenta)

🧫 dshb.biology.uiowa.edu/anti-vasa
🧫 dshb.biology.uiowa.edu/1B1

4. Autophagy/proteostasis/metabolism

5. ECM/ Basement membrane

From the meeting so far, here’s what seems in demand in the fly field; tell me I’m wrong.

1. Organelle markers
ER, Golgi, endosomes, lysosomes, mitochondria.

2. Post translational modifications
phospho-ERK, phospho-AKT, phospho-JNK, ubiquitin chains.

3. Cell-type markers
Glia, intestinal cells

Registration is OPEN for the 2026 Santa Cruz Meeting on Developmental Biology!!! Please spread the word!

@mads100tist.bsky.social @socdevbio.bsky.social @bsdb.bsky.social @xenbase.bsky.social @isdb.bsky.social @devbiol.bsky.social @the-node.bsky.social

scdb2026.sites.ucsc.edu

Picture of Sarah

Talk slide title with images of testis defects

If you're at #Dros26 and are interested in how cells change shape & move during morphogenesis & how different classic cadherins influence different cell behaviors, check out the talk by @saraheclark.bsky.social in Cell Biology: Cytoskeleton, Organelles, & Trafficking II Sat 9:15 🧪

Check out our booth at #Dros26!

#Dros26 Day 1

Comment below your favorite DSHB antibody!

📸 Drosophila brain labeled with DSHB anti-Bruchpilot in blue
🧫 dshb.biology.uiowa.edu/nc82

🔎 Spotted in Neurobiology of Disease!
Researchers from #OhioStateUniversity and @wrightstateu.bsky.social used DSHB's anti-NF-M [2H3] and anti-SV2A mAbs in a study on a new SCN8A epileptic encephalopathy mouse model that depicts seizures and motor impairments seen in patients.

🔎 Spotted in #ScientificReports by @natureportfolio.nature.com!
Researchers from #NTUsg used DSHB's anti-CD8A mAb in a study on the role of ARL13B on ciliogenesis and related signaling pathways.

📄 tinyurl.com/2f86jfum

🧫 dshb.biology.uiowa.edu/CPTC-CD8A-1

Spotted in @plosone.org!
Researchers from Quillen College of Medicine and @hubrechtinstitute.bsky.social used DSHB's anti-Krt8 [TROMA-I] mAb in a study on Lgr5-positive duct cells in von Ebner’s glands as potential stem cells for regenerating posterior tongue taste buds in mice.

Cooking up the next wave of Drosophila antibodies with Lori Wallrath 🧫🔬

Last day to visit DSHB at booth 1210! Don't miss out!

#CellBio2025 #uiowa @ascbiology.bsky.social @embo.org

@dshb-antibodies.bsky.social is returning to ASCB #CellBio2025 stop by and check out what's new at booth 1210

🔎 Spotted in Naunyn-Schmiedeberg's Archives of Pharmacology!
Researchers from International University of Health and Welfare, Narita Campus used DSHB's anti-LAMP-1 [1D4B], anti-NCAM [AG1], and anti-synaptic-vesicles [SV2] mAbs in a study on mapping the Neuropeptide Y Y₁ receptor in the mouse brain.

🔎 Spotted in #FASEBJournal!
Researchers from @pullman.wsu.edu and @ohsunews.bsky.social used DSHB's anti-β-tubulin [E7] mAb in a study on the effects of Intralipid enhancing fetal sheep adipose growth.

📄 tinyurl.com/3zt6762j

🧫 dshb.biology.uiowa.edu/E7_2

With the HCR™ HiFi Encoder, @dhouston.bsky.social (@uiowabiology.bsky.social) ran two @dshb-antibodies.bsky.social mouse IgG1 antibodies directly from supernatant and achieved robust double-labeling using HCR™ Gold IF.

🔬 Ready to try HCR™ Gold IF? Get a free Starter Kit here: tinyurl.com/37dfdenf

🔎 Spotted in @natbiotech.nature.com!
Researchers from @hubrechtinstitute.bsky.social, among others, used DSHB's anti-integrin-β1 [AIIB2] mAb in a study on how an antibody derivative (scTS2/16) enhances human epithelial organoid growth.

📄 tinyurl.com/yu36mt6m

🧫 dshb.biology.uiowa.edu/AIIB2

low-mag (5x) lateral view of a Xenopus tadpole; magenta is anti-skeletal muscle marker [12/101] staining the somites; green is anti-keratan sulfate [MZ15] staining notochord, both mouse IgG1s labeled with 546 and 647 encoders, respectively. Semi-cleared in 80% TDE.

Thanks to the folks at @HCRimaging for their nifty HCR HiFi encoders: "quantitative signal amplification of
1˚ antibodies for same-isotype multiplexing"
🔑@DSHB_antibodies can be tagged as supernatant, allowing mulplx wo/purification --> here are 2 trusted IgG1s used together

Highlight: Genome Duplication in a New Zealand Snail Holds Clues for the Persistence of (A)sexual Reproduction Why does sexual reproduction persist in so many species? This is a fundamental question in biology that, remarkably, remains a mystery. Sexual reproduction

Nice highlight of new genome paper. ❤️ emphasis on leadership of ECR Potamomics team members @kylemcelroy.bsky.social @chelseahiggins.bsky.social @jsharbro.bsky.social Genome Duplication in a New Zealand Snail Holds Clues for the Persistence of (A)sexual Reproduction: academic.oup.com/gbe/article/...

I am so proud & happy to see our Potamo genome assembly finally out there in the world! The process ended up being way more challenging than expected, but for some really cool reasons. ❤️🐌🧬
TLDR: Surprise! A VERY recent WGD.
academic.oup.com/gbe/article/...
Photo: C. Böck, ILIM, Mondsee, Austria)

How do four-eyed fish see above & underwater? 🌤️🌊 Our new preprint reveals how Anableps rewired its retina for dual vision- evolution at work 👁️👁️ Kudos to @perezlouise.bsky.social @josanesousa.bsky.social @keylapruett.bsky.social + team!
🔗 tinyurl.com/3a8r9xy5

🔎 Spotted in #Virulence by @tandfresearch.bsky.social!
Researchers from #WSU Floyd College of Medicine used DSHB's anti-α-tubulin [AA4.3] mAb in a study on how the GCN-2 pathway triggers stress responses in C. elegans during infection.

📄 tinyurl.com/3anhfuhm

🧫 dshb.biology.uiowa.edu/AA4-3

🐸 Xenopus laevis 💚 The frog that shaped modern developmental biology! ✨ Xenopus embryos have revealed how the body axis forms, cells choose their fate, and tissues self-organize during early development 📹 Video by Bioquest Sudio and Xenbase #ModelMonday #DevBio

🔎 Spotted in The Journal of Translational Medicine!
Researchers from Sun Yat-sen University, among others, used DSHB's anti-Gingipain/P. gingivalis [61BG1.3] mAb in a study on how P. gingivalis drives cervical cancer metastasis.

📄 tinyurl.com/yzjhb6sx

🧫 dshb.biology.uiowa.edu/61BG1-3

🔎 Spotted in Molecular Genetics and Genomics!
Researchers from @ur-ctsi.bsky.social and @ucsandiego.bsky.social used DSHB's anti-HP1 [C1A9] mAb in a study on how CRIF1 suppresses tumors by opposing Ras signaling in Drosophila.

📄 tinyurl.com/4uscst5c

🧫 dshb.biology.uiowa.edu/C1A9

UMAP of single nucleus RNAseq dataset of Polypterus fin regeneration

Hey Regen folks 👋 Excited to share that our single-nucleus RNA-seq dataset for Polypterus fin regeneration is now live at the Broad Institute portal! Dive in here: tinyurl.com/5bck22jx
Big thanks to the team for making this happen—looking forward to seeing what you all discover!

🔎 Spotted in @natcomms.nature.com!
Researchers from @uwmadison.bsky.social, among others, used DSHB's anti-MHC [MF 20] mAb in a study on how deleting ICAM-1 improves the immune resistance of stem-cell grafts.

📄 tinyurl.com/2s3pzheu

🧫 dshb.biology.uiowa.edu/MF-20

🔎 Spotted in Autophagy by @tandfresearch.bsky.social!
Researchers from #NationalTaiwanUniversity used DSHB's anti-α-Spec [3A9], anti-dlg [4F3], and anti-spitz mAbs in a study on Rab27’s role in regulating autophagy and lifespan in Drosophila.

Novel monoclonal antibodies against the C-terminal HEAT domain of Huntingtin biorxiv.org/content/10.110…

Nice collaboration between @DSHB_antibodies , @LabScribbles and @thesgconline to make and characterize anti-HTT mAbs

🔎 Spotted in Comparative Biochemistry & Physiology Part A!
Researchers from @umassamherst.bsky.social and @utaustin.bsky.social used DSHB's anti-NKA-α-subunit [a5] mAb in a study on how red drum larvae adapt acid excretion to survive ocean acidification.