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Simon Michaeli

@michaelilab

Plant and cell biologist. Interested in πŸ”¬ organelles, membrane trafficking, autophagy, πŸŒΏπŸ‚ senescence, πŸ‘ ripening, learning, teaching and much more πŸͺ https://www.michaelilab.com/

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20.11.2024
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Latest posts by Simon Michaeli @michaelilab

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An unconventional Rubisco small subunit underpins the CO2-concentrating organelle in land plants In many algae, photosynthesis is boosted by biophysical CO2-concentrating mechanisms, which pack the CO2-fixing enzyme Rubisco into liquid-like organelles called pyrenoids. Engineering C3 crops with a...

Our work on #hornwort #pyrenoids is finally out in @science.org! πŸŽ‰ We uncovered how hornworts pack their Rubisco into pyrenoids and successfully recreated them in Arabidopsis. A key step toward engineering more efficient photosynthesis in crops @btiscience.bsky.social www.science.org/doi/10.1126/...

05.03.2026 20:03 πŸ‘ 124 πŸ” 63 πŸ’¬ 6 πŸ“Œ 7
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Considering my book "The Tree of Life" for introductory evolution class? I have a few complimentary copies to evaluate. Please DM me. And please repost.

Review in Science described it as "..a fantastic informal introduction to a course on evolution.. recommended it to my first-year students".

06.03.2026 09:52 πŸ‘ 15 πŸ” 6 πŸ’¬ 0 πŸ“Œ 0
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Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress Cryo–electron tomography reveals how mitochondria reorganize their translation and folding systems under proteostatic stress.

Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress

www.science.org/doi/10.1126/...

08.03.2026 15:04 πŸ‘ 10 πŸ” 3 πŸ’¬ 1 πŸ“Œ 0
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Nanodomain-localized formin gates symbiotic microbial entry in legume and solanaceous plants Colonization of plant roots by symbionts requires substantial morphodynamic reorganization. Examples are actin-scaffolded microcompartments called infection pockets formed during root nodule symbiosis...

Happy to share our latest work in collaboration with the lab of @pengbo10.bsky.social. Here, we describe that a formin protein mediates the polarity switch from root hair to infection thread growth during symbiotic interactions.
www.science.org/doi/10.1126/...

08.03.2026 15:49 πŸ‘ 57 πŸ” 27 πŸ’¬ 4 πŸ“Œ 1

Congrats Alibek, Yasin et al

24.02.2026 06:38 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

Something tells me I should go to the membrane contact sites session...

24.02.2026 05:58 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Sun πŸ”† is rising over the bay of Eilat/Aqaba and the 2nd day of ILANIT 2026 is coming up.

24.02.2026 04:44 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 1
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We had a fab lab trip last week 🌻

21.02.2026 13:15 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Long in the making, but happy to present the Chlamydomonas chlororibosome!
Cryo-ETπŸ”¬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv πŸ“–: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n 🧡

10.02.2026 08:35 πŸ‘ 154 πŸ” 54 πŸ’¬ 4 πŸ“Œ 6
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#transgenerational transmission of a core #microbiome that protects against downy mildew from soil to #phyllosphere

Fantastic and important study by @jellespooren.bsky.social @rlberendsen.bsky.social
@cornepieterse.bsky.social
academic.oup.com/ismej/advanc...
#plantscience

14.02.2026 17:32 πŸ‘ 48 πŸ” 23 πŸ’¬ 2 πŸ“Œ 1
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New review from P Genschik’s team in
@jxbotany.bsky.social 🧬 A fresh look at the huge diversity and functions of plant F-box proteins.
πŸ‘‰ Open questions & new perspectives for 🌱 biology.
▢️https://academic.oup.com/jxb/advance-article-abstract/doi/10.1093/jxb/erag074/8475508?redirectedFrom=fulltext

14.02.2026 18:16 πŸ‘ 12 πŸ” 12 πŸ’¬ 0 πŸ“Œ 1

"The most uncomfortable part for me is that I no longer think that scientific research will resolve the crises that we face."

"I keep doing research not because I think it will save us, but because it would be a form of surrender to stop seeking understanding."

14.02.2026 00:18 πŸ‘ 13 πŸ” 6 πŸ’¬ 1 πŸ“Œ 0

Beautiful #preprint on a novel regulator of plant #autophagy from the @cnaumann123.bsky.social lab. How do plants regulate #autophagosome size??? ER-PM contact sites and Can1 seem to be key! www.biorxiv.org/content/10.6...

14.02.2026 10:23 πŸ‘ 11 πŸ” 5 πŸ’¬ 0 πŸ“Œ 0
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Our own ‏Dorothy Matiki‏ presented her research (on autophagy in πŸ… ripening) impressively and earned her M.Sc. title! πŸŽ‰πŸΎ

Soon Dorothy will meet the @weizmanninstitute.bsky.social Doctoral studies committee to (hopefully) approve her joining the lab of Prof. ‏Michal Sharon‏.
All the best Dorothy!

14.02.2026 10:14 πŸ‘ 4 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Recruitment of bifunctional regulator thermospermine to methylated ribosomes directs xylem fate Polyamines are often associated with ribosomes and are thought to stabilize their integrity. In Arabidopsis, the polyamine thermospermine (tSpm) affects xylem cell fate. tSpm induces translation of SU...

In @science.org this week, thermospermine affects ribosome methylation, which in turn regulates translation of xylem development proteins www.science.org/doi/10.1126/...

#plantscience

13.02.2026 09:43 πŸ‘ 30 πŸ” 20 πŸ’¬ 1 πŸ“Œ 1
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Apusomonad rhodopsins: A new family of ultraviolet to blue light–absorbing rhodopsin channels | PNAS Apusomonads are sediment-dwelling bacterivorous protists that are sister to all Opisthokonta. They have been found to show a negative phototactic r...

Apusomonad rhodopsins: A new family of ultraviolet to blue light–absorbing rhodopsin channels www.pnas.org/doi/full/10....

11.02.2026 09:33 πŸ‘ 11 πŸ” 6 πŸ’¬ 1 πŸ“Œ 0
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#SaturdayVibes

17.01.2026 13:55 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
A CK2-FBXW11 kinase-E3 ubiquitin ligase cascade is a metabolic sensor regulating Tryptophan 2,3-dioxygenase stability Small molecules toggling the ubiquitin-proteasome system (UPS) are powerful regulators of protein degradation. Yet, mechanistic knowledge of how endogenous ligands gate UPS decisions remains rudimentary. Here, we define control of UPS access to Tryptophan-2,3-dioxygenase (TDO2), which converts the essential amino acid tryptophan (Trp) to N-formylkynurenine. When Trp concentrations are limiting, TDO2 is degraded to avert tryptophanemia. Using CRISPRi screening and biochemistry, we identify a CK2-FBXW11 kinase-E3 ligase cascade that generates and recognizes tandem TDO2 phosphodegrons when not protected by Trp. Trp binding to an exosite safeguards TDO2 from phosphorylation-dependent ubiquitylation. Effects of Trp analogs on CK2-FBXW11-dependent ubiquitylation indicated that the indole, amino, and carboxylate groups are necessary for substrate shielding. Cryo-EM reveals how these moieties order a region proximal to the phosphodegrons; without Trp, this segment is flexible, enabling phosphorylation-coupled ubiquitylation. Overall, our data uncovered an endogenous small molecule allosterically stabilizing its own metabolizing enzyme through protection from a phosphorylation-ubiquitylation cascade. ### Competing Interest Statement B.A.S. is a member of the scientific advisory boards of Proxygen and Lyterian. The other authors declare no competing interests. Max Planck Society, https://ror.org/01hhn8329 European Union, ERC AdvG, UPSmeetMet, 101098161 to BAS Boehringer Ingelheim Fonds, https://ror.org/00dkye506

New year, new preprint! 🎊

We are excited to share our recent work on #E3 ligase regulation in #metabolism!

www.biorxiv.org/content/10.6...

#ubiquitin #targetedproteindegradation #chemicalbiology

1/6

13.01.2026 13:49 πŸ‘ 43 πŸ” 17 πŸ’¬ 1 πŸ“Œ 5
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a black background with the word idiocracy written in white letters . ALT: a black background with the word idiocracy written in white letters .

Dear @dfg.de So, I will spend months writing a grant and you will let people judge it with AI tools that are trained on predatory journals like Frontiers, MDPI or alike that are practically not peer-reviewed??? Is this how you will select cutting edge science??? #idiocracy

08.01.2026 15:30 πŸ‘ 27 πŸ” 12 πŸ’¬ 4 πŸ“Œ 0
Fig. 1 (shortened, full legend in paper): Complexity of gynoecium development shown with confocal microscopy imaging. (A) Meristematic stages of floral development in which the inflorescence meristem (IM) is observed; from stage 3, the sepal primordia (asterisks) are observed; in stage 4, the developing sepals (asterisks) partially cover the floral meristem (FM); and in stage 5, the FM is completely covered by the sepals. (B) Stage 6 in which the gynoecium is established with the appearance of the primordium of the gynoecium. (C) In stage 7, the middle and lateral domains can be differentiated (marked by dotted lines). (D–H) Longitudinal view of the gynoecium from stage 8 to 12. At stages 8 and 9, the gynoecium grows as a hollow tube and the valves and young replum can be seen. (F and G) Longitudinal view of stage 10 and 11 gynoecia showing the replum and valves. (H) Mature stage 12 gynoecium showing the stigma, style, and ovary, the latter formed by the valves and replum.

Fig. 1 (shortened, full legend in paper): Complexity of gynoecium development shown with confocal microscopy imaging. (A) Meristematic stages of floral development in which the inflorescence meristem (IM) is observed; from stage 3, the sepal primordia (asterisks) are observed; in stage 4, the developing sepals (asterisks) partially cover the floral meristem (FM); and in stage 5, the FM is completely covered by the sepals. (B) Stage 6 in which the gynoecium is established with the appearance of the primordium of the gynoecium. (C) In stage 7, the middle and lateral domains can be differentiated (marked by dotted lines). (D–H) Longitudinal view of the gynoecium from stage 8 to 12. At stages 8 and 9, the gynoecium grows as a hollow tube and the valves and young replum can be seen. (F and G) Longitudinal view of stage 10 and 11 gynoecia showing the replum and valves. (H) Mature stage 12 gynoecium showing the stigma, style, and ovary, the latter formed by the valves and replum.

🌸 Flowering Newsletter Review 🌸

LΓ³pez-GΓ³mez et al. review the role of protein–protein interactions in flower and fruit development and discuss the latest techniques to study them.

πŸ”— doi.org/10.1093/jxb/...

#PlantScience πŸ§ͺ @defolter-lab.bsky.social

09.12.2025 13:23 πŸ‘ 8 πŸ” 7 πŸ’¬ 0 πŸ“Œ 0
Defence-mediated phloem restriction of a plant virus facilitates insect transmission - Nature Communications How plant viruses are restricted to the phloem are long-standing questions in plant virology. The paper shows PENETRATION3 activity and callose deposition confine plant viruses to the phloem, which fa...

Why are so many plant viruses phloem-restricted? New work from Xueping Zhou’s lab reveals how this tissue tropism is determined in a geminivirus β€” and why phloem confinement can turn out to be an evolutionary advantage! www.nature.com/articles/s41... πŸŒ±πŸ¦ πŸš·πŸ…ΏοΈπŸ›«πŸŒ±

26.12.2025 11:39 πŸ‘ 28 πŸ” 12 πŸ’¬ 0 πŸ“Œ 0

Our work now on @jxbotany.bsky.social. Make sure you add some Cas13a fluorescent virus detection to your Christmas lights ✨️

24.12.2025 17:58 πŸ‘ 10 πŸ” 5 πŸ’¬ 0 πŸ“Œ 0
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Dynamic ASK1 proximity networks uncover SCF-dependent and noncanonical roles in ABA and drought adaptation Plants rely on rapid proteome remodeling to withstand fluctuating environmental conditions, yet how the ubiquitin system dynamically coordinates these multilayered responses remains unclear. Here we d...

Excited to share our latest work to close out 2025: Dynamic ASK1 proximity networks uncover SCF-dependent and noncanonical roles in ABA and drought adaptation🌱 With @justinwwalley.bsky.social , we map how ASK1/SKP1 dynamically rewires protein networks during stress www.biorxiv.org/content/10.6...

25.12.2025 20:49 πŸ‘ 4 πŸ” 4 πŸ’¬ 1 πŸ“Œ 0
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Researchers developed a #CRISPR-based method that's user-friendly, extraction-free, 15 min protocol for on-site #virus #detection using a portable fluorescence viewer and a mobile #phone camera directly from RNA of several crop species, including #tomato, #cucumber, and #rapeseed. πŸ‘‰ bit.ly/4p6Jo8t

24.12.2025 17:47 πŸ‘ 4 πŸ” 2 πŸ’¬ 1 πŸ“Œ 2

A 1.25 A!!! cryo EM structure of rubisco.

24.12.2025 11:15 πŸ‘ 23 πŸ” 9 πŸ’¬ 0 πŸ“Œ 0
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Christmas πŸŽ„ and Hanukkah πŸ•Ž celebration in Hala's (Dr. Khamesa-Israelov) place with all group members. What a treat πŸ₯‚πŸ»πŸ°

18.12.2025 13:41 πŸ‘ 4 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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Raffinose induces autophagy to promote the growth of Arabidopsis Thaliana - BMC Plant Biology Background Plant growth depends on the integration of environmental signals and nutrient availability. Under stress conditions, growth is often attenuated to prioritise defense, creating a trade-off b...

Excited to share our work about autophagy and raffinose. So proud of Sahar Magen for doing a great job and grateful for all the people who helped bring this work to light.
link.springer.com/article/10.1...

18.12.2025 16:32 πŸ‘ 5 πŸ” 4 πŸ’¬ 0 πŸ“Œ 0
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Christmas πŸŽ„ and Hanukkah πŸ•Ž celebration in Hala's (Dr. Khamesa-Israelov) place with all group members. What a treat πŸ₯‚πŸ»πŸ°

18.12.2025 13:41 πŸ‘ 4 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0

Organelles do NOT have a single uniform pH.
And if you think they must, because β€œprotons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧡 1/n

17.12.2025 00:46 πŸ‘ 518 πŸ” 221 πŸ’¬ 28 πŸ“Œ 31
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The Clavel Group is recruiting a new postdoc for two possible projects dealing with plant-virus interactions and selective autophagy! More (wordy) details below β¬‡οΈπŸŒ±πŸ¦ 

16.12.2025 17:15 πŸ‘ 33 πŸ” 38 πŸ’¬ 0 πŸ“Œ 1