Our study reveals that a fascinating complex composed of Mrx6, Mam33, and Pim1 acts as a gatekeeper, controlling #mitochondrial DNA copy number by regulating Lon-protease–dependent substrate turnover in #yeast.
Big thanks to all collaborators and co-authors involved!
Happy reading, everyone!
Happy to share that the last first-author paper from my doctoral research was featured on the cover of @narjournal.bsky.social academic.oup.com/nar/issue/54/2
It was exciting to paint the cover artwork, reflecting how our research combined wet-lab experiments with inputs from structural predictions.
Our new paper is out 🎉 in @narjournal.bsky.social
A fascinating complex composed of Mrx6, Mam33, and Pim1 controls #mtDNA copy number by regulating Lon-dependent substrate turnover in #yeast.
Congrats to @simonrecyclable.bsky.social, all co-authors and collaborators!
academic.oup.com/nar/article/...
Our study on the molecular function of translational activators for mitochondrial protein synthesis is published:
rdcu.be/eSWxT
A great collaboration with @stirlingchurchman.bsky.social and @sshaolab.bsky.social with structural work from our @pelleeas.bsky.social
Funded by @kawresearch.bsky.social
I found these immune cells just finished mitosis but their midbody is still yet to be resolved. How cool? If you zoom in to the midbody, you can see its classical shape just like in the textbooks. I love these little details.
Online now - the Review "Interconnectivity of mitochondrial protein biogenesis and quality control" from @mitolab.bsky.social et al.
#ProteinImport #Proteaes #Proteolysis #Proteostasis
Read it here: authors.elsevier.com/sd/article/S...
🏅We congratulate 🌟Nils Bertram🌟 @lmumuenchen.bsky.social, on winning a FEBS Letters #PosterPrize at the #EMBOFEBSproteostasis meeting, held in Espoo, Finland! 🎉👏👏👏
📸 Photo: Nils with our Managing Editor, Dr. Duncan Wright
I'm thrilled to share that our story is now out in Science Advances! 🎉 We use quantitative imaging to map the mito central dogma, define translation hubs in the mitochondrial matrix, and show that they're replaced by Mitochondrial Stress Bodies (MSB) when mtRNA processing is perturbed 1/4
Spatial analysis of mitochondrial gene expression reveals dynamic translation hubs and remodeling in stress | Science Advances www.science.org/doi/10.1126/...
Thank you, Arun! 😀
🚀 Excited to share our latest work on Mrx6 and mtDNA copy number regulation, now available on @biorxivpreprint.bsky.social ! We uncover how Mrx6 and Mam33 interact with the Lon protease Pim1 to control substrate degradation. 🔬 Check it out here: www.biorxiv.org/content/10.1... #mitochondria #mtDNA
Special thanks to Giada and Serena from the LMU MS facility, as well as Lea and Hannes @herrmannlab.bsky.social for their pulldown experiment with catalytically inactive Pim1. Also grateful for the Dean’s support in making this research public! Thanks to @osman-lab.bsky.social for the experience!
It was a particular pleasure to supervise and include undergraduate students Charlotte, Sebastian and Romina in our exciting journey through the project.
Excited to share our new preprint on Mrx6, who binds the Lon protease Pim1 N-terminal domain through a bipartite motif to confer selective substrate specificity and regulate mtDNA copy number! Looking forward to all your feedback and follow-ups! A huge thank you to everyone who contributed!
Mrx6 binds the Lon protease Pim1 N-terminal domain to confer selective substrate specificity and regulate mtDNA copy number https://www.biorxiv.org/content/10.1101/2025.02.14.638270v1
Akshaya Seshadri and Anjana Badrinarayanan @anjbadri.bsky.social @ncbsbangalore.bsky.social @tifrscience.bsky.social show that exonuclease action of replicative #polymerase gamma drives damage-induced #mitochondria #DNA clearance. New mechanism of mtDNA clearance.
www.embopress.org/doi/full/10....
Mitochondria (top) and microtubules (bottom) in a cell videoed through a microscope. #Science #Biology #CellBiology #microscopy
The cover image portrays a human composed of proteins who is examining their health status (represented by the ECG monitoring interface) with the assistance of artificial intelligence (represented by the chip and binary codes).
Something you don't see every day!
Digitizing the human proteome in >53,000 individuals with 15-year follow-up and, with machine learning, unraveling nearly 500 disease-causing proteins
www.cell.com/cell/fulltex...
open-access
Ridgeline chart showing the distribution of global daily air temperature differences from the pre-industrial reference period (1850-1900), for every year between 1940 and 2024. Each individual year resembles a hill, shaded in a darker shade of red and further to the right for warmer years. The trend is clearly towards warmer years, with 2024 standing out as first year above 1.5C.
NEW: 2024 has just been confirmed as the warmest year on record, and the first to breach the 1.5C threshold.
We used a ridgeline (Joy Division inspired) chart to visualise daily temperature anomalies since 1940.
2024 clearly stands out with 100% of its days above 1.3C and 75% above 1.5C.
Looking for some holiday reading material? Our latest paper identifying a new player in mitochondrial fission is now out @jcellbiol.bsky.social! A wonderful early present to see this now online!
rupress.org/jcb/article/...
One big advantage of laser induction is you can cause pearling whenever you want, in just the mitochondrial tube that you want, so it is very precise and controllable. Combined with STED imaging it has been possible to get some beautiful images of cristae and nucleoids during pearling
The transient nature of pearling makes it hard to detect - in normal cells you might need to sit and watch one spot for a long time and then, if you blink, you'll miss it
He found that pearling was ubiquitous, occurring in every cell type that he has looked at, including: U2OS, Hek293, COS7, RPE1, Jurkat, primary human fibroblasts primary CD8 T-cells, iPSC derived neurons, and budding yeast (shown here).
Mitochondria consist of networks of cylindrical tubes, right? Not necessarily! - in our new preprint, @gavsturm.bsky.social investigates how mitochondria transiently adopt a beads-on-a-string morphology
www.biorxiv.org/content/10.1...
Stoked to share TWO preprints on mitochondrial pearling! 🎉
We uncover how spontaneous #mitochondria pearling drives #mtDNA nucleoid distribution (doi.org/10.1101/2024...), and
@gavsturm.bsky.social
et al. dissect the biophysics behind it (doi.org/10.1101/2024...). Details below! 🔬🧵🧪
Pearling Drives Mitochondrial DNA Nucleoid Distribution https://www.biorxiv.org/content/10.1101/2024.12.21.629917v1
EXTRA! EXTRA! Read all about it! Latest SGD newsletter! www.yeastgenome.org/blog/sgd-new...
- AlphaFold
- YeastMine
- Community wiki
- SGD’s new GENETICS publication
- New yeast microPublications
- Alliance of Genome Resources 7.4
- Give a Gift/Support SGD
- Upcoming conferences
- Happy Holidays!
Delighted to share our new preprint on the cell biology and genome of Stentor pyriformis, a giant ciliate that maintains Chlorella endosymbionts in surface-associated microtubule baskets.
www.biorxiv.org/content/10.1...
Membrane blebs forming from the plasma membrane of a cell videoed through a microscope. If the bleb gets big enough, mitochondria, enter and explore. The cell is at the bottom where many mitochondria (bright signal) surround the nucleus (lack of signal). #Science #Biology #CellBiology #microscopy
