LifeCanvas Technologies

Data courtesy of Joe Luchsinger, Paige Hoffman, Caroline Mrejen, Jing Zhou, Kartik Pattabiraman, @dandoylephd.bsky.social, & @josemald.bsky.social 🔬 🐭 🧠

Studying neural circuits is all about connectivity. So why cut up your sample? This #FluorescenceFriday, we're highlighting some mesmerizing 3D neural circuit data scientists have generated with our sample processing techniques and #SmartSPIM imaging on whole mouse brains.

For @drmbede.bsky.social, whole-brain mapping opened up a world of discoveries. Read our convo to learn more about Michelle’s research, how to set up a new #neuroscience lab, and the importance of scientific community during challenging times: bit.ly/4rIgq0P

Everything you ever wanted to know about spinocerebellar development - all in one place!

So much remains to be discovered, from specification, to axon guidance, to circuit formation.

Such fun assembling what we know and framing future questions.

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These results from over 231 regions of interest provide an important reference for testing Alzheimer's drugs in preclinical trials. (3/3)

@davidashbrook.bsky.social
@dukemedschool.bsky.social
@uthsc.bsky.social
@edinburgh-uni.bsky.social

The team, led by Drs. Yuqi Tian & Kathryn Hornburg, found:
- Significant swelling in the neocortex, hippocampus, and amygdala
- Corresponding shrinkage in the thalamus, brainstem, and white matter
- Higher variation in females than males
- Notable changes in fear and memory
(2/3)

We know #Alzheimers disease drastically alters brain structure. But are there structural benchmarks we can use to test potential drugs? A new study combines MRI & #SmartSPIM microscopy to map brain-wide structural effects of mutations associated with Alzheimer's: bit.ly/46AAYzK 🧵 (1/3)

This #FluorescenceFriday is brought to you by a newly validated alpaca 🦙 Biotium nanobody for GFAP, which is pre-conjugated to a photostable CF dye & easy to multiplex. Sign up for our newsletter to get early updates & protocols for new applications: bit.ly/3MIZecf @biotium.bsky.social

How do you set up a new #neuroscience lab? 🤔 We caught up with @drmbede.bsky.social about being a new PI @ufmbi.bsky.social & why she chose to bring LifeCanvas along on her next big scientific journey. Check out this video for a peek at our convo, and stay tuned for the whole interview on our blog!

This week we're spotlighting Dr. Amberlene De La Rocha, who completed her recent dissertation in Dr. X. William Yang's lab at UCLA. Amberlene’s work introduces two new genetic tools for studying #Alzheimers in mice. Learn more: escholarship.org/uc/item/8sn2...

#HappyFluorescenceFriday #microscopycommunity - join the conversation with the T&E working group on core operations, community building, and strategies for sustainable growth.

Learn More: buff.ly/zMOwyg8

How long does it take to get #FluorescenceFriday data from an organoid on your microscope? With #DALISPIM, it only took us 53 seconds per channel (MAP2 in cyan, and Pax6 in yellow) – and it can be even faster! Learn more: bit.ly/49pqjKd

Next month, we'll be sharing 3D data from our pipeline at @mpfneuro.bsky.social's Sunposium, a conference focusing on basic neural circuit research. Come chat with us to learn how whole-brain circuit mapping can further your investigations 🌞 Learn more and register by 2/23: mpfi.org/events/sunpo...

In order to visualize MGE progenitor cell migration, the authors used LifeCanvas whole-brain mapping. This #SmartSPIM video shows extensive spread of GFP+ cells derived from MGE progenitors 60 days post-transplantation. Read the paper: bit.ly/4bNm0tB (4/4)

They also found that transplanting medial ganglionic eminence (MGE) progenitor cells into C1qa-deficient mice mitigated behavioral defects but did not abolish seizures, showing that interneuron dysfunction is not solely responsible for seizure pathology. (3/4)

The team found that C1qa KO leads to behavioral abnormalities, while C1qa germline deletion is associated with cortical synaptic dysfunction and reduction in SST-expressing interneurons. (2/4)

This #FluorescenceFriday comes from Scott Baraban's lab at UCSF: a new paper by Joseane Righes-Marafiga et al. sheds light on how dysregulation in the C1qa gene, which has been linked to neurological disorders like #epilepsy, affects inhibitory neural circuits. (1/4) 🧵

Also thank you to @andystoneimaging.bsky.social for shouting out #SmartSPIM in your talk on Brandeis' Light Microscopy Core Facility!

@michael-syglass.bsky.social @brandy-syglass.bsky.social

We had a great time sharing 3D data from our pipeline at the Massachusetts Life Sciences Center's Convening of the Cores! Thanks to Sony and syGlass for making our data pop on the Spatial Reality Display. MA researchers, check out LifeCanvas tools at cores near you: bit.ly/3O8SgO0

This video shows a 3D rendering of vCATCH-mediated afatinib-yne binding in a 1-week-old mouse, created from #SmartSPIM data. Microscopy data like these will be crucial for developing the next generation of targeted treatments for systemic diseases. Read the full paper: bit.ly/4c5kTFV (3/3)

vCATCH enables robust, homogenous click labeling throughout entire mice. The team combined vCATCH with SmartSPIM #lightsheet imaging to visualize whole-body binding of covalent cancer drugs throughout intact juvenile mice, as well as the torsos of adult mice. (2/3)

What if we could see 3D #biodistribution of cancer drugs at the cellular level... throughout an entire model animal? 🧐 A new paper from Li Ye's lab @scripps.edu, Zhengyuan Pang & Verina Leung et al. introduce a technique for doing just that. (1/3) 🧵

This #FluorescenceFriday we're spotlighting #organoids! Unlike 2D cultures, organoids provide spatial context for biological processes & cellular interactions. DALISPIM lets you study these systems in both high res & high throughput, which is essential for improving their translational relevance 🧫🔬

An overhead view of a dissected rat muscle resting on grid paper.

The same view of the muscle as Image 1, but the muscle is translucent and grid lines are visible through it.

A fluorescence micrograph of the rat tibialis anterior muscle, stained to differentiate the long nerve fibers from the dot-like neuromuscular junctions.

A higher magnification of Image 3.

The innervation of the tibialis anterior muscle in a rat has never been so clear! Processed with SmartBatch+ by the UIC/Structural Circuits Core, for @greisinglab.bsky.social in the UMN School of Kinesiology. Red = nerves, yellow = neuromuscular junctions. #3DThursday @lifecanvastech.bsky.social

We’re excited to announce our collaboration with the Laboratory of Systems Pharmacology (LSP) at @harvardmed.bsky.social, led by Professor Peter Sorger, to investigate solid human tumors in 3D. Read the full press release for more info: bit.ly/4qoHPDy @harvardlab.bsky.social #CancerResearch

Why is this rainbow mouse brain important for #neuroscience ? 🌈 You can see neural activity (green) alongside other features like cortical excitatory neurons (red) and nuclei (blue) throughout the entire brain. Multiplexed 3D data lets us glimpse neural processes in context. #FluorescenceFriday

Fluorescent image of a mouse brain, pseudo colored with cyan, magenta and gray

I cleared this brain last year with the @lifecanvastech.bsky.social SmartBatch and imaged with their SmartSPIM and I am pretty proud of it. As a computational guy, I was low key shocked that it came out as well as it did on the first try bc I forgot how to pipette

Want to see 3D #lightsheet data like never before? Visit our table at the Massachusetts Life Sciences Center's Convening of the Cores! Experience light sheet data on Sony's Spatial Reality Display, a next-generation glasses-free 3D display. Register for free by the end of this week: bit.ly/4sxfsFr

The ideal #MicroscopyMonday combination: high-res data, low-effort protocol. Get a close-up look of cortical neurons in this segment of mouse brain hemisphere, imaged with #DALISPIM at 9X magnification in a 12-well plate.