Luis Bonet-Ponce

Thanks!

Quick proteomics question: we want to use an exogenous biotin blocking scavenger for a TurboID experiment. We tried Biolock but is very inconsistent in our hands. Does anyone have another suggestion?

Cells come in many shapes and sizes, with diverse physiological functions. But how do #organelles and their interaction networks remodel during #differentiation of stem cells into different cell types? Here’s what we discovered about neuronal differentiation: 1/13

Thrilled to share that the first part of my PhD work from @cohenlaboratory.bsky.social lab is now on bioRxiv! www.biorxiv.org/content/10.6... Check out the threads below:

Our new paper's out: FidlTrack—structure-aware single-particle tracking benchmarks/boost SPT fidelity

With it we resolve with sub-organelle res. e.g. BACE1 amyloidogenic APP cleavage #Alzheimers, ER exit events, map nanobody binding in realtime in ER/organelles 🔬🧠#SingleMolecule

rdcu.be/e3Ris

New paper in @jcb.org! We found a new regulator of p62 phase separation, autophagy, and antioxidant signaling. It is an E3 ligase adaptor called SHKBP1, but the effect is via direct p62 binding, not ubiquitination. Congrats to Lin Luan & team! @weillinstitute.bsky.social

An intracellular meteor shower. EB3 comets tracking growing microtubule plus-ends in a cultured cell.

Check out our newest findings on how #lysosomes are generated from #endolysosomes. Great work from @samit2104.bsky.social ! @biozentrum.unibas.ch , @unibas.ch #cellbio #cell #organelles
Ca2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites www.biorxiv.org/content/10.6...

🙏🙏 to entire team esp co-lead Tim Bartels. So proud of @seppedeschepper.bsky.social brilliant first postdoc who now leads his own at VIB. 🙏🙏🙏to @chanzuckerberg.bsky.social for supporting the project from early pilot phase. Contact us if u want to explore next steps in PD gut/brain neuroimmunology!

🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!

ER exit sites mediated by the COPII adaptor sec24D selectively recruit lipid raft-preferring proteins for rapid ER export Nature Communications - Through synchronized cargo traffic experiments, the authors explore the role of raft partitioning in ER efflux. Raft-preferring cargos show specific preferences for ER exit...

Excited to share a new story that transformed how we think about membrane domains in the ER!

rdcu.be/eR7Cn

ER exit sites mediated by the COPII adaptor sec24D selectively recruit lipid raft-preferencing proteins for rapid ER export

Wondering what’s so new and exciting? Skytorial below!

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DNA nanodevices detect an acidic nanolayer on the lysosomal surface - Nature Cell Biology Tan and colleagues develop DNA nanodevices to detect the pH of the lysosomal outer surface, observing an acidic layer generated by TMEM175 that regulates lysosome positioning in response to changes in...

Beyond thrilled to share my PhD work out today in @natcellbio.nature.com , uncovering a nanoscale acidic environment around lysosomes that controls their mobility through proton signaling.🥳🥳🥳

Great thanks to my advisors and excellent peers for helping me！

www.nature.com/articles/s41...

LLOMe has long been used to study lysosomal damage, yet how it works has remained a mystery.
Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes – revealing a new paradigm for lysosomal failure.

🔗 doi.org/10.64898/202...

#CryoET

Continuum architecture dynamics of vesicle tethering in exocytosis Data from complementary imaging techniques were integrated into a model that resolves the dynamic exocyst ensemble and membrane architecture during exocytosis, an essential cellular pathway. Sec18 med...

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🥳 our #exocyst paper out in @cp-cell.bsky.social, led by Marta, Sebas & @sasmeek.bsky.social in collab with @jonasries.bsky.social, @cmanzo.bsky.social & Castaño-Díez labs #Quantitative_Cell_Biology

Continuum architecture dynamics of vesicle tethering in exocytosis
www.cell.com/cell/fulltex...

We are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉

🚨 Exciting news: Ciarán Butler-Hallisse's work on microtuble turnover is out! with help from @harrisonyork.bsky.social 💪 Look at those pretty neurons 👇😀

The Reck-Peterson lab at Weill Cornell Medicine is seeking a technician or staff scientist to work on Aspergillus projects. Broad background in fungal biology and techniques required. Bioinformatics skills preferred. Find out more at: reckpetersonlab.org.

This movie shows lysosomes (orange) and keratin (gray) in a cultured cell over 10 minutes.

Pre-pore and cis pores formed by full length perforin-2 during antigen escape from early phagosomes

Time for a new pre-print! How do dendritic cells control pore formation in their own endocytic compartments?

Our work shows that the unique transmembrane anchor of perforin-2 does not protect DC membranes, but instead it enables antigen escape during cross-presentation

doi.org/10.64898/202...

Damage done, tubules on - Studying membrane damaged-induced lysosomal tubulation  - FocalPlane Damage done, tubules on - Studying membrane damaged-induced lysosomal tubulation  - Default

Grateful to @focalplane.bsky.social for inviting us to write about our last paper on lysosomal tubulation during the stress response (rupress.org/jcb/article/...)!

focalplane.biologists.com/2025/12/11/d...

A proximity map of RAB GTPases delineates roles for RAB14 in EARP complex and UHRF1BP1 endosomal recruitments Communications Biology - Proximity labeling proteomics of RAB GTPases identifies an array of neighboring RAB proteins. Functional validation studies reveal links between RAB25 and DENND6A, or...

It is a nice way to end the week: our improved RAB GTPase BioID map now online! www.nature.com/articles/s42... We hope it will be useful to the community, and we're happy to share any reagents!

BigVolumeBrowser: a new 3D multi volume/mesh/point clould (SMLM) data viewer Hello everyone, I’d like to share with you another 3D viewer for FIJI, BigVolumeBrowser (full documentation link). It‘s a first initial public release, so there is still space for improvements. Le...

Finally, the first public release of #BigVolumeBrowser, so after teasers, you can try it yourself. For details, please check the announcement post (1/2)
forum.image.sc/t/bigvolumeb...

New insights into the activation of the most elusive and (so far) the least understood human inflammasome: NLRP6 is a sensor of endolysosomal damage

Excited to announce, the 93rd Biochemical Society Harden Conference will be on "Endosomal-lysosomal network dysfunction in neurodegeneration". Come and join us in early September on the beautiful south coast of England. Lots of speaker slots available from submitted abstracts!

An enzymatic cascade enables sensitive and specific proximity labeling proteomics in challenging biological systems - Nature Communications Proximity labeling allows proteomic mapping of subcellular structures. Here, Sroka et al. develop in situ APEX activation (iAPEX) to overcome limitations of labeling specificity and H2O2 toxicity, app...

Thrilled to share "in situ APEX activation" (iAPEX), a proximity labeling technology for subcellular #proteomics applied to primary #cilia. iAPEX uses an enzymatic cascade, in which a D-amino acid oxidase locally produces H2O2 to activate ascorbate peroxidase.
www.nature.com/articles/s41...

Check out our @ascbiology.bsky.social #CellBio2025 Minisymposium on ER Homeostasis. With THIS speaker lineup, this event will be as dynamic, transformative and productive as the ER itself!
So don’t 𝗦𝗧𝗥𝗘𝗦𝗦, 𝙩𝙧𝙖𝙣𝙨𝙡𝙤𝙘𝙖𝙩𝙚 yourselves over and join us to 𝗡𝗘𝗧𝗪𝗢𝗥𝗞 in world of the ER. (All PUNS intended.)

Mamdani: "New York will remain a city of immigrants, a city built by immigrants, powered by immigrants, and as of tonight, led by an immigrant."

I agree. I would say too that it is particularly difficult if you have little ones, as their well-being is also somewhat dependent on the grant/publication crapshoot.

Those are most of my days! You are doing great, just keep going!

🚨Our collaboration with @centriolelab.bsky.social & @gautamdey.bsky.social is out today in @cp-cell.bsky.social
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky

www.cell.com/cell/fulltex...