Amer Alam

#ABCA #ABCA7 #ABCtransporter #Alzheimers

#ABCD1 #VLCFA #cryoem

#ABCtrabsporter #AnnualReviews #membranetransport

Our Human ABC transporter review with the boss, Kaspar Locher

www.annualreviews.org/docserver/fu...

Structures of the human peroxisomal fatty acid transporter ABCD1 in a lipid environment - Communications Biology Le et al. present cryo-EM structures of the peroxisomal very long chain fatty acid (VLCFA) transporter ABCD1 in phospholipid nanodiscs in a nucleotide-bound conformation open to the peroximsomal lumen...

Earlier work from our lab on human ABCD1 structures

www.nature.com/articles/s42...

Cryo‐EM structures of human ABCA7 provide insights into its phospholipid translocation mechanisms | The EMBO Journal image image ABC subfamily A transporter ABCA7 mediates phospholipid extrusion from membranes. This study provides insights into its molecular mechanism based on cryo‐EM structures in lipid and deterg...

Repopulating the feed with our group's earlier work. Structures of human ABCA7 in a lipid environment:

www.embopress.org/doi/full/10....

Use of Bicelle-Generated Lipid Bilayer Vesicle Nanoparticles for In Vitro Measurement of Lipid Intermembrane Transport Herein, we describe a straightforward, easy method for generating stable lipid bilayer vesicle nanoparticles and show their usefulness for efficient in vitro tracking of lipid intermembrane transfer a...

Happy to have contributed to this excellent Methods in Molecular Biology chapter by Rhoderick Brown's lab, now in print.

link.springer.com/protocol/10....

Structural insights into binding-site access and ligand recognition by human ABCB1 multi drug exporter
Amer Alam and colleagues
www.embopress.org/doi/full/10....

Structural insights into binding-site access and ligand recognition by human ABCB1 | The EMBO Journal imageimageABCB1-dependent multidrug resistance is a major cause of reduced efficacy of drug-based therapy. The cryo-EM-derived structures of ABCB1 presented here reveal a previously uncharacterized au...

www.embopress.org/doi/full/10....

Our latest manuscript on human #ABCB1 / #MDR1 / #p-glycoprotein / #pgp / #p-gp structures, No stabilizing antibodies or mutations. 4 conformations including a novel apo state in saposin lipid nanoparticles out in The EMBO Journal @embojournal.bsky.social