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International Society for Protein Termini (ISPT)

@ispt-proteinterm

Non-profit society of scientists devoted to understanding the molecular mechanisms and biological role of protein termini in plants, animals, fungi and bacteria. Conference every second year. Website: https://proteintermini.org/

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Latest posts by International Society for Protein Termini (ISPT) @ispt-proteinterm

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Co-translational control of protein stability and quality in plants Abstract. Proteostasis relies on the coordinated control of protein synthesis, folding, modification and degradation, and an increasingly clear picture is

My review “Co-translational control of protein stability and quality in plants” is now online at @jxbotany.bsky.social, in which I describe how co-translational processing and ribosome-associated quality control together establish protein stability and fate early in synthesis. tinyurl.com/5dhhz9h6

24.02.2026 09:45 👍 41 🔁 24 💬 0 📌 0
Protein Termini 2026 – International Society for Protein Termini (ISPT)

8 days left to register | 28 invited speakers | Keynotes from F. Ulrich Hartl, Roland Beckmann and Michael Rapé | #chaperones #degradation #ubiquitin #cryoEM #acetylation #lipidation #ribosomes #proteins
proteintermini.org/meeting/

23.02.2026 09:16 👍 4 🔁 2 💬 1 📌 0
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Actin N-terminal maturation can be blocked by a butynamide stereoprobe which acts as an ACTMAP inhibitor. Preprint by Xiong et al. Benjamin Cravatt and Bruno Melillo. @ispt-proteinterm.bsky.social
www.biorxiv.org/content/10.6...

23.02.2026 08:32 👍 9 🔁 5 💬 0 📌 0
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Join us in Palermo!
FEBS Workshop Protein Termini 2026: the power of protein termini across bacteria, plants, and animals—from ribosome biology to proteostasis and applications. Deadline: 3 March 2026
proteintermini.org/meeting
#ProteinTermini #Proteostasis #FEBS #EMBO @iubmb.bsky.social

16.02.2026 15:10 👍 12 🔁 8 💬 1 📌 0

Join us at the @crick.ac.uk for the 2026 meeting of the UK proteostasis community!
We especially encourage students and postdocs to attend and share their work. All talks (except the keynotes) will be selected from abstracts.

13.02.2026 13:55 👍 19 🔁 15 💬 0 📌 0

Surprisingly: "Conditional stability of HY5 through the ATE N-degron pathway regulates environmental responses in Arabidopsis thaliana".
The shining bounds of N-degron pathway influence expands! @charlene-kunaka.bsky.social www.biorxiv.org/content/10.6...

12.02.2026 08:58 👍 15 🔁 14 💬 1 📌 0
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Protein event of the year - sign up today! See you at beautiful Palazzo dei Normanni in Palermo for the FEBS 2026 Protein Termini Workshop.
#ProteinTermini #Proteostasis #ProteinModifications #StructuralBiology #PalazzoDeiNormanni #Palermo2026
proteintermini.org/meeting/

09.02.2026 20:49 👍 8 🔁 8 💬 1 📌 1
FEBS Workshop 'Protein termini 2026: From mechanisms to biological impact'

Remember to register for this year's Protein Termini FEBS Advanced course. Keynote by Roland Beckmann @beckmannlab.bsky.social "It takes more than two to tango: structural basis and coordination of co-translational N-terminal nascent chain modification" www.conferencecentral.org/webpage/view...

07.02.2026 15:49 👍 3 🔁 2 💬 0 📌 0
Functional divergence of the Arg/N-degron pathway between the crop Brassica rapa and the model plant Arabidopsis thaliana The ubiquitin-dependent Arg/N-degron pathway relates the stability of a substrate protein to the nature of its N-terminal amino acid residue or its biochemical modifications, with some N-terminal residues being recognized by specific E3 ubiquitin ligases, resulting in the ubiquitylation and degradation of the substrate protein. Work in the model plant Arabidopsis thaliana has shown that the Arg/N-degron pathway is a key regulator of plant responses to hypoxia, which can be either physiological or a stress in the context of waterlogging or submergence. The role of the Arg/N-degron pathway in hypoxia response is mediated via the oxygen-dependent degradation of group VII ETHYLENE RESPONSE FACTOR (ERFVII) transcription factors, which act as the master regulators of the hypoxia response program in plants. Analysis of Arabidopsis mutants for different enzymatic components of the Arg/N-degron pathway has also revealed its roles in the regulation of responses to other abiotic stresses (e.g. salt stress), as well as to pathogens. Although much has been learned from studies in Arabidopsis about the functions of the Arg/N-degron pathway, very little is known about this pathway in crops, including in Brassica crops such as oilseed rape, cabbage or turnip. To determine functional similarities and divergence of the Arg/N-degron pathway between Arabidopsis and Brassica crops, we isolated and characterized the first Arg/N-degron pathway mutants in Brassica rapa (turnip, pak choi), a diploid Brassica crop closely related to oilseed rape. We focused on two enzymatic components, namely the arginine-transferases ( ATE s) and the E3 ubiquitin ligase PROTEOLYSIS6 ( PRT6 ). Our results show both similarities and divergence of function for these Arg/N-degron pathway components in B. rapa compared to Arabidopsis. Specifically, ATE mutants in B. rapa arrest their development at the seedling stage, which contrasts with the mild phenotypic defects of the equivalent Arabidopsis mutants. Double mutant lines for two of the three PRT6 genes in B. rapa indicated a constitutive activation of hypoxia response genes at the transcriptional level, as shown in the single prt6 mutant in Arabidopsis. However, contrary to Arabidopsis, the B. rapa double mutants were more sensitive to waterlogging and hypoxia, and did not show differential response to salt stress or to biotic stress compared to the wild type. The functional divergence identified likely reflects variability in each species in the substrate repertoire and/or in the regulation of pathways or targets downstream of Arg/N-degron pathway substrates. Such differences could be driven by direct selective pressures at N-termini (e.g. gain or loss of a destabilizing N-terminal residue), or by species-specific proteases that may generate destabilizing neo-N-termini after cleavage. These similarities and differences highlight the difficulties in translating research findings from Arabidopsis to crops, even within the same plant family (Brassicaceae) and highlight the need to study pathways in crops. ### Competing Interest Statement The authors have declared no competing interest. Science Foundation Ireland, https://ror.org/0271asj38, 13/IA/1870, 20/FFP-P/8433 Irish Research Council, https://ror.org/051xex213, GOIPG/2017/2

Protein degradation in Turnip - an adapted Arg/N-degron pathway. Preprint from Brian Mooney et al. Emmanuelle Graciet lab. www.biorxiv.org/content/10.6...

30.01.2026 14:09 👍 6 🔁 5 💬 0 📌 0
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Ribosome-NAC collaboration: A regulatory platform for cotranslational chaperones, enzymes, and targeting factors Protein biogenesis requires the ribosome to collaborate with a diverse set of cotranslational factors that shape the fate of nascent chains. These int…

Ribosome-NAC collaboration: A regulatory platform for cotranslational chaperones, enzymes, and targeting factors. New review out in @cp-molcell.bsky.social from Elke Deuerling's lab. www.sciencedirect.com/science/arti...

28.01.2026 11:18 👍 6 🔁 3 💬 0 📌 0
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Complexity at the ribosomal polypeptide tunnel exit. The major N-terminal acetyltransferase NatA may form different complexes on the ribosome to facilitate protein maturation. By Marius Klein & Klemens Wild in Irmgard Sinning's lab BZH & Nina McTiernan in my lab. www.nature.com/articles/s41...

26.01.2026 11:24 👍 16 🔁 8 💬 0 📌 1
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This is how co-translational N-terminal myristoylation occurs on the ribosome. NMT1 acts together with NAC, but after the release of MetAP. Cryo-EM study by Timo Denk et al. @beckmannlab.bsky.social @giglionelab.bsky.social www.nature.com/articles/s41...

26.01.2026 11:00 👍 17 🔁 6 💬 0 📌 0
CUL4A-DDB1-DCAF10 is an N-recognin for N-terminally acetylated Src kinases - Nature Communications Cells depend on early protein modifications for proper function. Here, the authors show that when Src-family signaling kinases lack their typical myristoylation, an alternative acetylated start is det...

Intricate regulation of Src kinases via N-terminal modifications and specific degradation pathways. A novel degradation pathway involving the CUL4A-DDB1-DCAF10 E3 ligase found by Kremer et al. Tanja Bange lab @andrea-musacchio.bsky.social @ispt-proteinterm.bsky.social www.nature.com/articles/s41...

05.01.2026 13:34 👍 8 🔁 4 💬 0 📌 0
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Mechanism of cotranslational modification of histones H2A and H4 by MetAP1 and NatD NAC enables cotranslational N-terminal processing of histones H2A and H4 by recruiting MetAP1 and NatD at the ribosomal tunnel.

Last X-Mas, the ribosome gave you methionine,
but the very next day, MetAP took it away.
This year, to save histones from tears,
NatD gives you an acetyl group. ⭐️

Explore our latest paper with the Deuerling lab @uni-konstanz.de and Shu-ou Chan lab @caltech.edu!

www.science.org/doi/10.1126/...

20.12.2025 00:34 👍 46 🔁 17 💬 0 📌 0

Indeed, most Nt-acetylation events occur co-translationally in eukaryotes. Likely to assure an immediate and proper protein maturation, incl. folding, targeting & protection from Ubiquitin E3s. Also post-translational Nt-acetylation: actin (NAA80), transmembrane proteins (NAA60),++ & more to come🤓

11.12.2025 10:34 👍 1 🔁 0 💬 1 📌 0

Functional divergence of the Arg/N-degron pathway between the crop Brassica rapa and the model plant Arabidopsis thaliana https://www.biorxiv.org/content/10.64898/2025.12.04.692320v1

09.12.2025 06:02 👍 9 🔁 5 💬 0 📌 0
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HYPK promotes N-terminal protein acetylation through rapid ribosome exchange of NatA Huntingtin-interacting protein K (HYPK) promotes the activity of N-terminal acetyltransferase A (NatA), which cotranslationally acetylates 40% of the eukaryotic proteome. Kinetic and in-cell measurements revealed that HYPK acts as a ribosome exchange factor for NatA, enabling its access to and acetylation of the translatome.

Online Now: HYPK promotes N-terminal protein acetylation through rapid ribosome exchange of NatA Online now:

10.12.2025 16:19 👍 9 🔁 4 💬 0 📌 2
FEBS Workshop 'Protein termini 2026: From mechanisms to biological impact'

Just registered for FEBS advanced workshop Protein termini 2026 in Palermo #proteintermini2026 (?) Looking forward to a great meeting. @ispt-proteinterm.bsky.social
www.conferencecentral.org/webpage/view...

03.12.2025 14:05 👍 2 🔁 4 💬 0 📌 0
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We’ve made some new tools to manipulate N-recognins. Check them out in our preprint.
www.biorxiv.org/content/10.1...

29.11.2025 03:24 👍 18 🔁 13 💬 0 📌 0
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Want to assess N-terminal acetyltransferases without radioactive labelling or mass spec? Look here! @ispt-proteinterm.bsky.social @cellsignal.com

27.10.2025 20:25 👍 4 🔁 3 💬 1 📌 0
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See you at Palazzo dei Normanni in Palermo for the 2026 Protein Termini Conference | Co- and post-translational modifications | Proteolytic processing and degradation pathways | Microbes, plants, animals #ProteinTermini2026 #Proteostasis #ProteinModifications #PalazzoDeiNormanni #Palermo2026

26.10.2025 18:59 👍 7 🔁 7 💬 2 📌 1
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Daniel Gibbs Lab Welcome to the lab website of Professor Daniel Gibbs @ University of Birmingham

We are advertising a PhD project investigating a cellular mechanism that ensures protein quality control during mRNA translation in plants. Please get in touch on here or via email if you are interested and I can provide further details!

Lab website: sites.google.com/site/danielg...

14.10.2025 14:07 👍 38 🔁 41 💬 0 📌 0
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NAA60 facilitates LRRC8A- and LRRC8D-mediated platinum drug uptake - Communications Biology Genome-wide CRISPR/Cas9 screening identifies the N-terminal acetyltransferase NAA60 to facilitate cis- and carboplatin drug sensitivity, most likely by neutralizing Nt acetylation of the LRRC8A/D VRAC subunits regulating substrate permeability.

Uptake of anti-cancer drugs depends on N-terminally acetylated anion channels | @ispt-proteinterm.bsky.social www.nature.com/articles/s42...

07.10.2025 11:03 👍 5 🔁 2 💬 0 📌 0
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A Cell-Potent Bisubstrate Inhibitor to Probe NatD Acetyltransferase Activity N-terminal acetyltransferase D (NatD) is a highly selective enzyme that acetylates the α-N-terminal amine of histones H4 and H2A, which share the SGRGK motif. Elevated NatD expression has been observe...

A Cell-Potent Bisubstrate Inhibitor to Probe NatD Acetyltransferase Activity | The inhibitor effectively reduced cellular Nα-acetylation on histone H4, leading to reduced migration in lung cancer cells | @acs.org ACS Chemical Biology @ispt-proteinterm.bsky.social pubs.acs.org/doi/10.1021/...

05.10.2025 12:06 👍 8 🔁 3 💬 0 📌 0
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Protein N-terminal acetylation regulates the growth of rice plants | #NAA30 #NatC #rice #acetylation www.sciencedirect.com/science/arti...

25.09.2025 09:45 👍 3 🔁 3 💬 0 📌 0
Methods in Enzymology | Volume 719: Protein Termini - Part B | ScienceDirect.com by Elsevier Read the latest chapters of Methods in Enzymology at ScienceDirect.com, Elsevier’s leading platform of peer-reviewed scholarly literature

Two new volumes of Methods in Enzymology with 29 chapters/papers on Protein termini methods and reviews are now out! Thx to all authors!🙌 @ispt-proteinterm.bsky.social #N-degron #prenylation #myristoylation #formylation #arginylation #oxidation #acetylation www.sciencedirect.com/bookseries/m...

25.09.2025 09:17 👍 6 🔁 3 💬 1 📌 0
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Developing leaves form a spatiotemporal oxygen gradient that is sensed through the oxygen sensing machinery and directs morphogenesis | New preprint from @daanweits.bsky.social and @syno2xis.bsky.social labs #N-degron #Cysteine #hypoxia @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...

23.09.2025 07:38 👍 6 🔁 3 💬 1 📌 0
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Anatomy and habitat shape the oxygen sensing machinery of angiosperms Hypoxia sensing via the Cys/Arg branch of the N-degron pathway (Cys-NDP) is central for flooding responses in plants, yet how evolutionary and ecological factors have shaped the core oxygen sensing me...

Anatomy and habitat shape the oxygen sensing machinery of flowering plants | new preprint from Licausi lab @syno2xis.bsky.social #hypoxia #N-degron @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...

18.09.2025 12:40 👍 8 🔁 5 💬 1 📌 0
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The generation and consequences of N-terminal proteoform diversity N-terminal proteoforms are protein variants with altered N termini that arise from RNA-driven processes, such as alternative promoter usage, splicing,…

New review on the generation and consequences of N-terminal proteoform diversity from Evan Morrison and Olivia Rissland | #N-degron #RNA @cp-cellreports.bsky.social www.sciencedirect.com/science/arti...

17.09.2025 08:09 👍 3 🔁 3 💬 0 📌 0
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Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of motor neurons and the cytoplasmic aggregation of misfolded proteins in the spinal cord...

Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform | Yong Tae Kwon lab | #N-degron www.biorxiv.org/content/10.1...

08.09.2025 11:07 👍 8 🔁 4 💬 0 📌 0