Tatiana Moiseeva

Thank you!!

Thank you!

Thank you!

Thank you:)

Thank you!

Thank you:)

Thank you!!

First NIH grant for the lab!! Very nice way to start the week :)

Congratulations!!

Students need to remember that Inigo Montoya method for emails and greetings:
"Hello, my name is Inigo Montoya. You killed my father. Prepare to die.”

Polite Greeting
Name
Relevant Personal Link
Manage Expectations

Keep it BRIEF.

New preprint🎉
Happy to share our collaborative manuscript, where we provide in vivo and genome-wide evidence linking MCM2-7 helicase activation, DNA extrusion to initial origin melting, thereby adding a new mechanistic layer to how eukaryotic DNA replication is initiated.

👉 doi.org/10.64898/202...

A fluorescent microscopy image of two green nuclei. One of them has five micro nuclei that look like toes

My cell has (is?) a foot!

How DNA secondary structures drive replication fork instability DNA secondary structures, such as hairpins, cruciforms, triplexes, G-quadruplexes and iMotifs, are common, dynamic features that replication forks rou…

Read our latest work - a comprehensive review on how DNA secondary structures affect replication.

Very proud of Adi, Maria and Billie for their monumental effort. They've covered a large body of literature while proposing updated models.

Open Access in DNA Repair:
doi.org/10.1016/j.dn...

a model for TIMELESS positions at the active fork: one complex sits at the leading edge of the fork, while the other one is on the lagging strand ssDNA/RPA, between the Okazaki fragments

Ever wondered how TIMELESS/TIPIN can sit at the leading edge of the replication fork regulating its speed, but also bind polymerases and ssDNA/RPA in replication stress? We have an idea how it might work! Check out our new preprint, any feedback is highly appreciated!
www.biorxiv.org/content/10.6...

Is it just me, or does it look like liberty is going to die in 2026, based on this coin?

Inhibition of lagging strand replication by G-rich telomeric DNA and the shelterin subunit POT1 https://www.biorxiv.org/content/10.1101/2025.11.13.688213v1

Check out our new biosensor technology to study DDR kinase signaling: ProKAS.

We combine:
-proteomics
-engineered peptide sensors
-a new concept of amino acid barcodes

ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data.

Just published today: rdcu.be/ePNo0

From MRI to Ozempic: breakthroughs that show why fundamental research must be protected In these financially straitened times, funders must recognize that great discoveries often arise from work that was looking for something completely different.

In praise of fundamental research
Our editorial this week argues that I n these financially straitened times, funders must recognize that great discoveries often arise from work that was looking for something completely different
🧪
@nature.com
www.nature.com/articles/d41...

Human RPA is an essential telomerase processivity factor for maintaining telomeres Telomerase counteracts telomere shortening by repeatedly adding DNA repeats to chromosome ends. We identified the replication protein A (RPA) heterotrimer as a telomerase processivity factor critical ...

Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...

The eukaryotic replisome intrinsically generates asymmetric daughter chromatin fibers DNA replication is molecularly asymmetric, due to distinct mechanisms for lagging and leading strand DNA synthesis. Whether chromatin assembly on newly replicated strands is also asymmetric remains un...

Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up!
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)

For now our university thinks it may be applied to all H1B holders at border crossing, they advise either to enter the US today before midnight, or stay put and not try

Oh, certainly not look kind. Just to iterate the fake "gold standard science" or something. They would still target specific universities, of course, but maybe generally all of them. I know, it is a weak argument, that's all i got

All I can do is hope at this time, there's not much else. Maybe giving universities a break could be a small performative gesture for them, it is such a tiny fraction of H1Bs🙈

My new senior scientist's H1B was approved yesterday, but now they can't come in. I am still hoping universities will be exempt🙈

Last evening President Trump issued a Proclamation titled Restriction on Entry of Certain Nonimmigrant Workers, which aims to block anyone seeking to enter the U.S. on an H-1B visa, unless a $100,000 fee has been paid by their employer. The best advice we can give at this time comes from outside counsel, Fragomen, Del Rey, Bernsen & Loewy, LLP. At this time, we recommend the following: (1) If you are currently in the United States in H-1B status, do not travel out of the country (even to Canada to Mexico) until further notice; (2) If you are currently outside of the United States with an H-1B visa, do everything you can to return before midnight ET tonight (Saturday), if possible; and (3) If you are outside of the United States and cannot get back by midnight ET tonight (Saturday), do not attempt to return until we know more about how the Order will be implemented.

When your incoming lab member's H1B was approved yesterday, but now they can't travel to the US...

a photo of me chatting with very nice other conference attendies, a glass in my hand, but my facial expression is very skeptical for some reason

Thanks CSHL for the photos, my facial expressions on them are priceless. I think this one is a keeper for replying to a lot of stuff on the internets...

absolutely, adding now!

A landscape showing green trees and some leaves changing colors, harbor water is seen down between the trees

Excited to be back at the CSHL meeting on DNA replication this week! Campus views are amazing as always, looking forward to all the science!

A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells Nature Communications - Determining how replication forks move across the human genome is critical for the effective use of agents that target replication stress. Here, the authors present...

Checkout our latest research in @natcomms.nature.com
rdcu.be/eBqBI A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells. A collaboration with Mike Boemo’s team

Mechanism of Rad51 filament formation by Rad52 and Rad55-Rad57 in homologous recombination Nature Communications - The mediator protein Rad52 promotes Rad51 binding onto RPA-coated DNA to initiate homologous recombination. Here, the authors show that Rad52 sorts Rad51 into monomers and...

How are Rad51 filaments formed during Homologous Recombination?

Jaigeeth Deveryshetty and Ayush Mistry describe the mechanism through the lens of Rad52, the yeast homolog of human BRCA2.

urldefense.com/v3/__https:/...