Warre Dhondt

Now published @cp-cellreports.bsky.social! Antibody-mediated TGF-β1 activation for the treatment of diseases caused by deleterious T cell activity: www.cell.com/cell-reports...
Fantastic collaboration & massive effort from Sophie Lucas Lab @ de Duve Institute. #CryoEM with @savvideslab.bsky.social.

In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells In eukaryotic cells, LINC complexes physically bridge the two nuclear membranes to cytoskeletal filaments, transmitting mechanical forces to the nucleus. Due to their dynamic and membrane-embedded nat...

Fantastic work by the group of @matteoall.bsky.social : a SUN5:Nesprin3 complex at the head:tail junction of sperm cells. 🤯 #teamtomo

www.biorxiv.org/content/10.1...

This work is the result of a fantastic collaboration with@matteoall.bsky.social!
@warred.bsky.social, @a-dsantos.bsky.social , @alisterburt.bsky.social , @tom-hale.bsky.social, @mrclmb.bsky.social,

We are excited to announce the first in-cell structure of a LINC complex within a native nuclear envelope at subnanometer resolution!! #In-cell cryo-ET + #atomistic MD simulations! @tomdendooven.bsky.social et al!!
www.biorxiv.org/content/10.1...

This structure is absolutely _nuts_. Lipids are captured marching through the tunnel

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries The mechanistic understanding of the complex between ALK kinase and its cytokine ligand ALKAL2 has been complicated by reports of diverse stoichiometries (2:1 vs 2:2). This study reanalyzes cryo-EM da...

Delighted to share our reanalysis of cryo-EM data in EMPIAR-10930, now at:

journals.plos.org/plosbiology/... ;

revealing the ALK-ALKAL2 assembly with 2:1 stoichiometry and resolving the conundrum introduced by the originally reported 2:2 complex. ALK/LTK-cytokine signalling getting clearer!

In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:

The @structurabio.bsky.social team have been preparing *Amazing* workshops and tutorials on all things related to cryo-EM image analysis, demonstrating deep talent in communication in addition to software design.
If you're interested in learning about cryo-EM do yourself a favour and have a look!

I get it, thanks!

In the sense that the variations/structures do not have proper (Boltzmann) weights?

I sometimes forget how flexible proteins are. It becomes really apparent when you use NMR states for an animation. That poor cofactor is getting pushed around a lot 😅

#sciart #blender3d #biocatalysis

Structure of mTORC complex by cryoEM showing electron density maps

An amazing cryoEM structure of the membrane-assembled lysosomal mTORC complex from the Hurley lab. @jimhurley.bsky.social
www.biorxiv.org/content/10.1...

If pombe wants to be taken seriously as an organism it needs to ferment a better beverage