WRGordonlab-UMN

July 1…. Notch your average day. 11 year anniversary of the lab. 11th PhD student graduating this week. Promoted to full professor. 4 year DGS term is OVER! Feeling lucky despite all of the chaos. #gordonlabpuns

Fat cadherin cleavage releases a transcriptionally active nuclear fragment to regulate target gene expression The conserved atypical cadherin fat (ft) controls cellular processes such as growth, planar cell polarity, and mitochondrial function, in organisms ranging from fruit flies to mammals. Working at the ...

Fat cadherin cleavage releases a transcriptionally active nuclear fragment to regulate target gene expression.

New preprint from Helen McNeill and colleagues
doi.org/10.1101/2025...

Ok bringing up an old debate- I am writing my R35 second renewal, and it’s hard to squeeze it all in 6 pages. Both margins justified saves me 6 lines but my husband claims it’s hard to read. So: left- or both- margins justified for grants????? Please help!

Cas9 gets an upgrade! Check out our new paper in The CRISPR Journal: bit.ly/hiNLS-Cas9 Eric Noel (who is on the job market!) created Cas9 constructs w/ nuclear localization signals (NLS) in the backbone, boosting genome editing activity in T cells, as seen by editor @srishtisahu.bsky.social #CRISPR

Elastic properties of leukemic cells linked to maturation stage and integrin activation Acute myeloid leukemia (AML) remains challenging to cure. In addition to mutations that alter cell functioning, biophysical properties are modulated by external cues. In particular, membrane proteins ...

Elastic properties of leukemic cells linked to maturation stage and integrin activation
pmc.ncbi.nlm.nih.gov/articles/PMC...

Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading Cells sense mechanical cues and convert them into biochemical responses to regulate biological processes such as embryonic development, aging, cellula…

Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading
www.sciencedirect.com/science/arti...

2. Great presentations start with the key message How to present science · Episode

Just listened to the second episode of Marloes ten Kate's "How to present science" podcast.

Very interesting insights, how to deliver the key message in the beginning to increase engagement as well as into the ABT model. (Although the Tesla references did not age well) (1/2)
open.spotify.com/epi...

Exploring the intersection of mechanobiology and artificial intelligence - npj Biological Physics and Mechanics npj Biological Physics and Mechanics - Exploring the intersection of mechanobiology and artificial intelligence

Nice review about the opportunities of implementing AI/ML in mechanobiology by Roger Oria, Kashish Jain and Valerie Weaver (didn't find them on bluesky yet):

www.nature.com/articles/s44...

U of Michigan @umich.edu set aside funds from central to support researchers with unexpected disruption in federal funding for up to 6 months.

Are any other institutions doing this? Seems like a good move.

#Medsky #NIH #federalfunding #researchfunding 🧪

record.umich.edu/articles/uni...

A computational pipeline for spatial mechano-transcriptomics - Nature Methods The authors present a computational framework that leverages mechanical force inference and spatial transcriptomics to enable analyses of the interplay between the transcriptomic and mechanical state.

🚨 Paper Alert 🚨
Absolutely delighted to share our last paper on "spatial mechano-transcriptomics" @naturemethods👇!!
www.nature.com/articles/s41...

Shaping epithelial tissues by stem cell mechanics in development and cancer - Nature Reviews Molecular Cell Biology Adult stem cells self-renew and differentiate to build, maintain and repair tissues. This Review discusses recent insights into the contribution of mechanical forces produced by epithelial stem cell a...

Our first 2025 paper!
We discuss in @natrevmcb.bsky.social how epithelial stem cells mechanically sculpt tissues, and how these forces are altered in cancer.
Great work from our former and current tumor mechanobiology experts Vince Fiore & @jorgealmagro.bsky.social 👏
www.nature.com/articles/s41...

ITGB1/FERMT1 mechanoactivation enhances CD44 characteristic stemness in oral squamous cell carcinoma via ubiquitin-dependent CK1α degradation - Oncogene Oncogene - ITGB1/FERMT1 mechanoactivation enhances CD44 characteristic stemness in oral squamous cell carcinoma via ubiquitin-dependent CK1α degradation

ITGB1/FERMT1 mechanoactivation enhances CD44 characteristic stemness in oral squamous cell carcinoma via ubiquitin-dependent CK1α degradation
www.nature.com/articles/s41...

Force transmission is a master regulator of mechanical cell competition - Nature Materials Differences in force transmission capabilities between competing cells create large stress fluctuation at their interface, resulting in upward forces and cell elimination, which might have implication...

@bladoux.bsky.social lab on mechanical cell competition

@naturematerials.bsky.social
@luanger.bsky.social, @andreasschoenit.bsky.social, Siavash Monfared!

www.nature.com/articles/s41...

Really cool work from friend and colleague Katie Galloway @mitcheme.bsky.social!

The hits just keep coming for this cohort. First f*cked by COVID and now these delays and obstructions. We need to pursue creative funding strategies and keep the pipeline flowing.

Protease-Containing Nanobodies for Detecting and Manipulating Intracellular Antigens Using Antiviral Drugs https://www.biorxiv.org/content/10.1101/2025.03.09.642226v1

Measuring and manipulating mechanical forces during development - Nature Cell Biology This Review discusses the recent advances in experimental approaches to interrogate the mechanical forces that mediate tissue deformations during development, highlighting the insights afforded at bot...

Happy to share our review on methodology for measuring and manipulating mechanical forces with specific focus on developmental biology! Congrats to authors @clemvilleneuve.bsky.social @mccreery.bsky.social and hats off to the community for developing awesome tools www.nature.com/articles/s41...

Feeling the force from within – new tools and insights into nuclear mechanotransduction Summary: This Review discusses new insights and technological advances in understanding how the nucleus contributes to the ability of the cell to translate mechanical stimuli into transcriptional resp...

Interested in nuclear mechanobiology, and how advanced genomic tools can shed light on how the cell nucleus might sense mechanical forces? Read our latest review by excellent Julien Morival and Anna Hazelwood, now published @jcellsci.bsky.social:
journals.biologists.com/jcs/article-...

Can you add me please?

Fast Antibiotic resistance-Based gene editing of mammalian cells with CRISPR-Cas9 (FAB-CRISPR) Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency causing low knock-in rates. We ...

Long in the making but our protocol for gene editing is online! FAB CRISPR is super easy and allows the generation of N and C terminal fusions with TurboID, SNAP, Halo and mStayGold. A single cloninf step to generate HDR templates! arxiv.org/abs/2502.12675 1/n

Villari G, et al. Luminescent sensing of conformational integrin activation in living cells. Cell Rep. 2025 Feb 17;44(2):115319. [Epub ahead of print] PMID: 39964812

Mechanical forces pattern endocardial Notch activation via mTORC2-PKC pathway. #DevBio
https://buff.ly/41e7Wnb

#CellMigration for #FluorescenceFriday 🧪🔬

If a metastatic #cancer cell is offered 2 paths it decides very fast, bc it does matter where it goes...

OTOH, if a non-cancer #cell is offered 2 options it takes some time to explore and decide.

More in the paper: www.nature.com/articles/s41...

My first post on this forum! 😄

I am happy to share that our #openaccess review on the #molecular mechanisms of #actin filament polymerization and dissasembly is now online @cp-trendscellbio.bsky.social

www.cell.com/trends/cell-...

Big thanks to my amazing colleagues in Dortmund

Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from gene expression and real-time fluorescence to modulation..

Well at least I know what an intein feels like :)

Would love to be added- we work on protein- dna bioconjugation via huh endonucleases

📯📯 Chemical Biology starter pack 📯📯
140 PIs & groups posting primary research in chemical biology.

Please reskeet (?) & reply to this post if you want to be added!
go.bsky.app/KLrTWoj

Let's make ChemBioSky even greater again!

#realtimechem #FluorescenceFriday #chemsky #chemtwitter #AgentOrange

Traction force microscopy is important tool for #mechanobiology!

Check our thread for some of the historic papers moving the technique forward!