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Tung Le

@tunglejic

Dad of two, Professor, Lister Research Fellow and Wellcome Investigator at the John Innes Centre. Interested in bacterial chromosome organization & segregation, plasmids, and phages. www.tunglelab.org

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07.11.2023
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Latest posts by Tung Le @tunglejic

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CauloCon 2026 A free, 3-day virtual meeting on all things Caulobacter and Alphaproteobacteria

Less than 24 hours until CauloCon starts! Last minute registration is still open if you want to attend.

caulocon.com

02.03.2026 19:27 πŸ‘ 5 πŸ” 3 πŸ’¬ 0 πŸ“Œ 2

Oh no, he is great, I had such a great first lab experience in his group!!! He will be missed!!!

25.02.2026 18:42 πŸ‘ 3 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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PhD Scholarship at Monash University - MACSYS Do cutting-edge research at the intersection of cryo‑EM, genome-wide profiling, and AI-driven modelling. Work with world‑class cryo-EM/cryo-ET imaging and AI tools to discover how the dangerous bacter...

Come do a PhD with me and @trevor-lithgow.bsky.social at @monashuniversity.bsky.social ! We’re at the leading edge of pathogen biology using cutting edge Cryo-EM and AI-driven genetic screens to uncover the mysteries of outer membrane biology. Reach out to me for a chat!

macsys.org/phd-scholars...

25.02.2026 06:48 πŸ‘ 19 πŸ” 18 πŸ’¬ 0 πŸ“Œ 1

Exciting line up of talks for CauloCon 2026 - includes keynotes from @brunlabcaulo.bsky.social @lamasonlab.bsky.social and @thanbichlerlab.bsky.social! Hear work from Isaac Payne and Trung Nguyen, 2 students in my lab, on Weds and Thurs. 🀩

23.02.2026 17:52 πŸ‘ 10 πŸ” 7 πŸ’¬ 0 πŸ“Œ 0

and money/philanthropic funding!

21.02.2026 11:21 πŸ‘ 1 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0

Great picture!!!

19.02.2026 16:32 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Cell-free genomics reveals fundamental regulatory principles of the Mycobacterium tuberculosis transcription cycle Tiers of gene regulation govern cellular life. The intrinsic activities of RNA polymerase (RNAP) constitute a primary tier, while direct modulation by…

Congrats Ruby, Bob, Liz, and team! www.sciencedirect.com/science/arti...

19.02.2026 16:21 πŸ‘ 8 πŸ” 3 πŸ’¬ 1 πŸ“Œ 2

so cool and impressive!!!

19.02.2026 16:31 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

striking graph!!!

16.02.2026 14:34 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Revitalizing actinobacteria research: an urgent response to the antimicrobial resistance crisis - Natural Products and Bioprospecting Abstract The crisis of antimicrobial resistance (AMR) is escalating while the antibiotic pipeline remains stagnant. Our bibliometric analysis of eight decades of literature reveals a critical imbalanc...

Research into filamentous actinomycetes, the antibiotic producers, is in steep decline. Loads of natural products chemistry research worldwide but not much aimed at understanding their biology and ecology. The latter is key to unlocking their specialised metabolism

link.springer.com/article/10.1...

16.02.2026 13:32 πŸ‘ 36 πŸ” 21 πŸ’¬ 2 πŸ“Œ 3

Many congratulations, Kelly!!!

10.02.2026 10:28 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
The bacterial RNA polymerase-associated CarD protein couples promoter activity to DNA supercoiling - Nature Communications The transcription factor CarD facilitates the activation of transcription in many bacteria and in Rhodobacter sphaeroides, CarD compensates for suboptimal promoter DNA sequences. Here, the authors sho...

In our most recent work David Forrest has discovered a widespread mechanisms linking transcription initiation to DNA supercoiling...

www.nature.com/articles/s41...

06.02.2026 15:36 πŸ‘ 24 πŸ” 14 πŸ’¬ 1 πŸ“Œ 0

Hey alpha aficionados - it's almost time for CauloCon 2026! This free, virtual meeting will feature talks from @brunlabcaulo.bsky.social @thanbichlerlab.bsky.social and @lamasonlab.bsky.social with opps for trainee talks. Register using the link below. πŸ‘‡

02.02.2026 18:58 πŸ‘ 16 πŸ” 12 πŸ’¬ 2 πŸ“Œ 0
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CauloCon 2026 A free, 3-day virtual meeting on all things Caulobacter and Alphaproteobacteria

CauloCon 2026 is coming! πŸ”¬βœ¨ March 3–5 (fully virtual + free). Keynotes by Yves Brun, Becky Lamason & Martin Thanbichler. Great chance for grad students & postdocs to present their work (work-in-progress welcome!). Submit an abstract by Sun Feb 8. Register + submit: www.caulocon.com

02.02.2026 18:54 πŸ‘ 13 πŸ” 11 πŸ’¬ 1 πŸ“Œ 2
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I am finally a proud owner of a Kamado β€œGreen Egg”!!! An excellent parting gift from @leahmcphillips.bsky.social before leaving the lab!!!

23.01.2026 17:02 πŸ‘ 16 πŸ” 0 πŸ’¬ 3 πŸ“Œ 0
A methylome-derived m6-dAMP trigger assembles a PUA-Cal-HAD immune filament that depletes dNTPs to abort phage infection Bacteria must distinguish phage attack from normal homeostatic processes, yet the danger signals that trigger many defence systems remain unknown. Here, we show that a PUA-Calcineurin-CE-HAD module from Escherichia coli ECOR28 confers broad anti-phage protection by binding Dam-methylated deoxyadenosine monophosphate (m6-dAMP) generated during phage-induced chromosome degradation. Ligand binding converts a preassembled PUA-Calcineurin-CE hexamer loaded with six HAD phosphatases into a polymerising filament. The filament acts as a high-flux dNTP sink through a two-enzyme cascade: HAD first dephosphorylates dATP to dADP, and Calcineurin-CE then converts dADP to dAMP. dNTP collapse halts phage replication and enforces abortive infection. Multiple mobile-element DNA mimic proteins block filament assembly, revealing a direct phage counter-defence. More broadly, our findings extend a conserved, cross-kingdom paradigm of immune filament assembly to nucleotide-depletion antiviral defence and suggest modified-nucleotide sensing by related PUA-Calcineurin-CE modules as a widespread, underappreciated bacterial strategy. ### Competing Interest Statement The authors have declared no competing interest. NIHR Southampton Biomedical Research Centre, https://ror.org/01qqpzg67, Postdoctoral Bridging Fellowship F.L.N. is supported by a Wessex Health Partners (WHP) and National Institute for Health and Care Research Wessex Experimental Medicine Network (NIHR WEMN), Seed fund National Institutes of Health, GM145888, U24 GM129539) Maloris Foundation Memorial Sloan Kettering Cancer Center, P30-CA008748 Simons Foundation, SF349247 New York State Assembly

Preprint out: We characterise PUA-Cal-HAD, a widespread bacterial antiphage defence family. An infection cue switches a preassembled complex into an immune filament that drains dNTPs via a coupled two-enzyme cascade, and phage DNA mimics can block filament assembly (anti-polymerisation).

17.01.2026 14:52 πŸ‘ 34 πŸ” 19 πŸ’¬ 1 πŸ“Œ 0

πŸ€£πŸ‘

08.01.2026 08:31 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

In two-week time, it will be 10 years of our group. I have not regretted once joining Mol Micro Dept and more widely the John Innes Centre.

13.12.2025 12:19 πŸ‘ 29 πŸ” 2 πŸ’¬ 0 πŸ“Œ 0
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Just got word that we are a go for the Bacterial Cell Biology and Development GRS and GRC at Southern New Hampshire University!

GRS will be on June 5-6, 2027

GRC will be from June 6-11, 2027

Block off your calendars Prokaryotic Cell Biologists!

More details to come! Please repost!

12.12.2025 14:58 πŸ‘ 61 πŸ” 37 πŸ’¬ 3 πŸ“Œ 2

Save the date! This will be an exciting meeting (+ seminar) for all bacteria lovers!!

12.12.2025 15:14 πŸ‘ 8 πŸ” 2 πŸ’¬ 0 πŸ“Œ 0

A legend!!! I look up to him every day since 2007- my former PhD supervisor!!!!

11.12.2025 20:29 πŸ‘ 6 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0
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🧡 2/7 But what about their distribution across wider bacterial diversity? And how do these systems maintain plasmids in bacteria that do not divide by binary fission, such as Streptomyces, which have a complex filamentous life cycle?

11.12.2025 13:29 πŸ‘ 5 πŸ” 1 πŸ’¬ 1 πŸ“Œ 0
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Stable inheritance of the Streptomyces linear plasmid SCP1 by dual ParABS partition systems Low-copy-number plasmids often rely on dedicated maintenance mechanisms, such as partitioning systems, to ensure stable inheritance across generations. These partition systems actively segregate siste...

πŸ“„ Delighted to share that the preprint of my main PhD work is out now!

πŸ‘ A huge thank you to everyone involved, @tommclean.bsky.social, Govind Chandra, Ngat Tran (both not on BlueSky) and especially my PhD supervisor @tunglejic.bsky.social πŸ‘

🧡 below!

www.biorxiv.org/content/10.6...

11.12.2025 13:25 πŸ‘ 25 πŸ” 15 πŸ’¬ 1 πŸ“Œ 1

Many congratulations, Tamara!!!

11.12.2025 13:19 πŸ‘ 0 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0

I have now read this - impressive work!!!

11.12.2025 13:18 πŸ‘ 0 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0

Check out this work by the amazing @leahmcphillips.bsky.social from during her PhD!!

11.12.2025 08:10 πŸ‘ 11 πŸ” 8 πŸ’¬ 0 πŸ“Œ 0

Stable inheritance of the Streptomyces linear plasmid SCP1 by dual ParABS partition systems https://www.biorxiv.org/content/10.64898/2025.12.10.693275v1

11.12.2025 05:16 πŸ‘ 10 πŸ” 5 πŸ’¬ 0 πŸ“Œ 1

Our study of the evolution of the ParB NTP binding domain across the Tree of Life is now published! An awesome collaboration with @tunglejic.bsky.social led by @jovanakaljevic.bsky.social at the John Ines Center. Thanks to editors and reviewers. www.pnas.org/doi/10.1073/...

05.12.2025 09:05 πŸ‘ 18 πŸ” 4 πŸ’¬ 0 πŸ“Œ 1
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Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation | PNAS Nucleotide triphosphate (NTP)-dependent molecular switches regulate essential cellular processes by cycling between active and inactive states thro...

Now published. Thank you very much to our collaborative team, and very supportive editors and reviewers!!!

www.pnas.org/doi/10.1073/...

04.12.2025 19:22 πŸ‘ 44 πŸ” 12 πŸ’¬ 0 πŸ“Œ 0
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Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation | PNAS Nucleotide triphosphate (NTP)-dependent molecular switches regulate essential cellular processes by cycling between active and inactive states thro...

Our preprint is now published in PNAS! This came together thanks to a great collaboration with Antoine Hocher and a strong team effort from the Le Lab. Thank you to the reviewers and to everyone who helped improve it. I hope ParB aficionados will enjoy it.

www.pnas.org/doi/10.1073/...

04.12.2025 20:10 πŸ‘ 35 πŸ” 18 πŸ’¬ 2 πŸ“Œ 1