I'm pretty skeptical about the finding of 2 of those papers for other reasons.
But wouldn't you expect the uncoating to be arfgap dependent rather than tethering?
I guess both need to happen simultaneously in rapid succession,
but why would VPS41 affect uncoating?
Corrine Smith and Steve royle's lab showed very nicely that the beta3 ear is insufficient to aid clathrin assembly despite the functional clathrin box in the hinge.
Figure 2C. Replacing B3 ear with B2 ear on the B3 hinge allowed clathrin assembly (notably not as good as AP2)
PMID: 34487371
Doesn't it just makes more sense that VPS41 is part of HOPS complex and facilitates teething/fusion of AP3 carriers by it's interaction with Delta hinge?
Structural basis of caveolin-driven membrane bending https://www.biorxiv.org/content/10.64898/2026.02.05.703862v1
That being said AP3, AP4 and AP5 all still lack structures on lipid bilayers and COPI hasn't been resolved in solution. So describing the conformational changes of the family remain incomplete but it's exciting to see almost all the pieces of this puzzle.
Now finally at least one structure of each of the 6 human AP complexes has been published!
We are finally getting close to holistically understanding the differences/intricacies in both recruitment and vesicle formation of this family.
The squid has already taken over C-wing!
Centrosome?
I have been wondering why this wasn't already solved for at least 4 years.
Glad to see it finally.
AP1 complex paper incoming?
This!
I have also seen papers show tenuous PAE plots and then state that it must be a "weak interaction" as if it's a metric of binding affinity.
Just in rough shape this particle reminds me of ฮณTuRC. Whats the localisation of these particles?
The mechanistic basis of cargo selection during Golgi maturation. From Rebecca Taylor, @katciazynska.bsky.social, @jggkaufman.bsky.social & @grigorytagiltsev.bsky.social at @mpibiochem.bsky.social with David Owen and Sean Munro's group @cellbiol-mrclmb.bsky.social | www.science.org/doi/10.1126/...
Back up and running now.
All to be improved on subsequently I am sure.
Same, it's pulled out some very exciting and unexpected interactors for me. However I find the coverage is somewhat patchy and there are some obvious misses
Yes. It was running fine a few days ago but went down mid search. My guess is too much user traffic.
Congratulations Stephan! Long time in the making. Beautiful work and great to finally have a function for RMC1.
Super interesting paper on the evolutionary origins of an strange complex of uncertain function.
And congrats on the preprint ofcourse!
Is that AP2?
Looking forwards to reading about it!
We have a preprint for you
EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer
By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...
Wow thanks for sharing. Slightly surreal seeing a structure from my PhD in a review.
I am thoroughly looking forwards to reading this!
Wow. I'm surprised the stage can generate so much force.
How did this happen?
Congratulations Zunlong!
I initially thought it was another COVID structure!
