Josh Tycko

The patient, KJ, reaching out after infusion of the CRISPR therapy, with a big smile! Photo credit Children's Hospital of Philadelphia

In a medical breakthrough, a team including IGI’s
@urnov.bsky.social & @giannikopoulosp.bsky.social created an on-demand #CRISPR therapy for an infant with a deadly gene mutation — developed, approved, and delivered to the patient in just 6 months.

Read more: ow.ly/G0Bg50VTonC

#RareDisease 🧬

Congratulations! Awesome paper. Promoter reprogramming is very cool to see

I am elated to share that our manuscript describing Variant-EFFECTS, a high-throughput technology we developed to precisely and quantitatively measure the effects of CRISPR-mediated edits on gene expression, is now published at @cellpress.bsky.social: authors.elsevier.com/c/1kxgiL7PXu...

Human-chimpanzee tetraploid system defines mechanisms of species-specific neural gene regulation A major challenge in human evolutionary biology is to pinpoint genetic differences that underlie human-specific traits, such as increased neuron number and differences in cognitive behaviors. We used ...

What contributes to human specific neural specification? Check out these two wonderful preprints from a wonderful scientist Ava carter! 1. www.biorxiv.org/content/10.1...; 2. www.biorxiv.org/content/10.1...

Stay tuned! third preprint is on its way!

Moving Beyond 300 Base Pairs | Twist Bioscience In a recent episode of the podcast series Lab Talk by The Scientist, guest speaker Josh Tycko’s excitement was infectious. Tycko, a postdoctoral researcher at Harvard Medical School, spoke about how f...

How Multiplexed Gene Fragments are Expanding What’s Possible in Molecular Biology!

@twistbioscience.com
@joshtycko.com

Moving Beyond 300 Base Pairs:

Our sun and planets form a tiny nucleus surrounded by the Oort cloud, a thick icy shell that has never been directly observed but is thought to provide a continuous source of comets

Other stellar systems can crash through the Oort cloud, and it could happen again in as little as 1-10 million years

Impressive study from Alex Stark lab identifying 3 new types of silencer elements in Drosophila

New TF binds a particular isolated motif in non-accessible sites to recruit G9a. Deletion of these elements can lead to upregulation of nearby gene

www.sciencedirect.com/science/arti...

Single-molecule states link transcription factor binding to gene expression - Nature A study uses single-molecule footprinting to measure protein occupancy at regulatory elements on individual molecules in human cells and describes how different properties of transcription factor bind...

www.nature.com/articles/s41...

Single-molecule states link transcription factor binding to gene expression

Deep insights into multiple facets of TF binding, accessibility & expression with biophysical models of single molecule data by Ben Doughty, Michaela Hinks, Bintu & Greenleaf labs

Deep review of an accelerating area in genome engineering - large insertions & deletions. The authors here really dug up what's known and nicely synthesized it.

It ends with a fun question: "Do we need most of our DNA at all, or could we delete 90% of it without any loss of function?"

Thanks Matias! Dig the “.bio” wonder if I can cop your style

Curious if and how paper sharing and summary threads differ on this site

Development of compact transcriptional effectors using high-throughput measurements in diverse contexts - Nature Biotechnology Improved effectors for CRISPRi/CRISPRa are developed following high-throughput screening of transcriptional domains.

New here and excited to share our new paper on transcriptional effectors! Interested to discuss context-specificity of gene regulation? Or how to get CRISPRi/a working in new contexts? 👋
www.nature.com/articles/s41...