Chris Mulligan

And now published as an Observation! Many congrats to @weeboont.bsky.social for discovering that AsmA superfamily proteins, YhdP/TamB/YdbH, localize to the cell poles in E. coli, curiously AFTER division has occurred! 1/3 #MicroSky
journals.asm.org/doi/10.1128/...

Fabian was quicker :) But yes, we have an open postdoc position in bacterial bioenergetics and membrane biology, using on B. subtilis as the model. Come join us! @cbcb-newcastle.bsky.social is a great place to work, and we are a friendly, international lab. Visa+IHS fees covered. #microsky

Structural elucidation of the hexameric MmpS4-MmpL4 complex from Mycobacterium tuberculosis https://www.biorxiv.org/content/10.64898/2026.01.07.698164v1

Thomas Lab @ York Gavin Thomas lab in York

Deadline 7th Jan. See researches pages of
@GavinHThomas
(thomaslabyork.weebly.com), Emily Flack (york.ac.uk/biology/peop...) & Martin Fascione (fascionelab.com)

My lab in the NIH Intramural Program (Bethesda, MD) will be recruiting postdoctoral fellows over the next year with flexible start dates. We work on nanoscale cellular imaging of the plasma membrane and related organelles. Please reach out if you’re interested. www.training.nih.gov/jobs/pdp-053...

Research Associate in Bacterial Transport Mechanisms at University of Kent Discover Research Associate in Bacterial Transport Mechanisms jobs and more in higher education on jobs.ac.uk. Apply for further details on the top job board.

And now advertised here too if you prefer:
www.jobs.ac.uk/job/DPX771/r...

🚨My lab is hiring a postdoc!🚨
If you’re interested in working out the mechanism and physiological impact of bacterial lipid transport processes then please apply!

Job advert is here: tinyurl.com/4swddfda

Get in touch by email (c.mulligan@kent.ac.uk) for informal enquiries

Please repost!
Thanks!

Postdoctoral Scholar Research The Reddy Lab at the University of South Florida (USF) is seeking a Postdoctoral Researcher to join our multidisciplinary team studying the structure, mechanism, evolution, and engineering of membrane...

My lab is hiring a postdoc! We have many exciting projects on membrane transporters at the interface of protein structure-function, bioinformatics, directed evolution, and protein engineering. Applications considered on a rolling basis, so submit ASAP. Please share!

jobs.usf.edu/hcmUI/Candid...

The Gonzalez Lab at Columbia University is seeking a scientific leader!

Join us in a Research Scientist position, driving single-molecule, structural, and biochemical studies of translation, translational control, and other RNA-based processes.

Apply: apply.interfolio.com/176949

Structural basis of undecaprenyl phosphate glycosylation leading to polymyxin resistance in Gram-negative bacteria - Nature Communications The study reveals the structure of ArnC, a membrane glycosyltransferase that modifies undecaprenyl phosphate with aminoarabinose (L-Ara4N), leading to polymyxin resistance. The structure of ArnC in tw...

I'm happy to share that our work describing the #cryoEM #structure of the glycosyltransferase ArnC from Salmonella enterica has been published in Nature Communications. #membrane #protein #PetrouLab

The article is open access and available at:
www.nature.com/articles/s41...

Description Please note that job descriptions are not exhaustive, and you may be asked to take on additional duties that align with the key responsibilities ment...

We're advertising for a bacteriology technician and a post-doc to work on AI-guided antibiotic development as part of a large multidisciplinary Fleming Initiative project:
www.imperial.ac.uk/jobs/search-...

www.imperial.ac.uk/jobs/search-...

Polymyxins slow down lateral diffusion of proteins and lipopolysaccharide in the E. coli outer membrane - Communications Biology Multi-scale molecular dynamics simulations reveal that polymyxins form polymyxin-protein aggregates upon associating with the E. coli outer membrane thereby reducing lateral mobility of outer membrane...

Our paper on polymyxin interacting with the E. coli outer membrane is out! Simulations by the very talented @dheerajprakaash.bsky.social www.nature.com/articles/s42...

To scale outer membrane protein island simulated at coarse-grained resolution with portions refined at all atom resolution.

If you or someone you know is looking for a post-doctoral position working on mycobacterial envelope biology, please get in touch. I am recruiting here at the University of Western Ontario in London, Ontario, Canada.

Bidirectional communication between nucleotide and substrate binding sites in a type IV multidrug ABC transporter - Nature Communications The communication hinge, found in type IV ABC transporters, forms a bidirectional communication between ATP- and substrate-binding sites. This is an unexplored allosteric pathway critical for function...

Super nice to wake up to the news that our paper on bidirectional communication in an ABC transporter has been selected as a Nature Communications Editors’ Highlight in “Structural biology, biochemistry and biophysics"! 🥳 🥳 🥳
www.nature.com/articles/s41...

@microverse.bsky.social

Structural characterization of the YbbAP-TesA ABC transporter identifies it as a lipid hydrolase complex that extracts hydrophobic compounds from the bacterial inner membrane In E.coli, three of the four Type VII ABC transporter systems have been structurally characterized. This study solves cryo-EM structures of the fourth Type VII ABC system, YbbAP-TesA, and suggests tha...

Type VII ABC transporters underpin many core activities in the bacterial cell envelope - including efflux, cell division and lipoprotein trafficking.

A new paper in PLOS Biology reveals YbbAP-TesA as a novel Type VII system in E. coli.

dx.plos.org/10.1371/jour...

Structural basis of lipopolysaccharide assembly by the outer membrane translocon holo-complex - Nature Communications The asymmetric distribution of lipopolysaccharide (LPS) on the surface of the bacterial outer membrane is essential and crucial for antibiotic resistance. Here, authors characterize the LPS translocon...

Very happy to share our work on lipopolysaccharide assembly by the Lpt complex, published today in @natcomms.nature.com
Fantastic collaboration with @raffaeleieva.bsky.social l and t @pstansfeld.bsky.social
Congrats to all authors, especially Haoxiang, Axel & Violette
🔗 doi.org/10.1038/s414...

Now properly published at @natcomms.nature.com with few additional experiments incl. demonstration of Daptomycin's ability to depolarise non-growing cells.
See the original preprint-thread for a summary of our findings.
www.nature.com/articles/s41...
#microsky

CCD2MD: A Suite of Packages for Preparing Co-Folded Outputs for Molecular Dynamics Simulations Protein–lipid interactions play a crucial role in the stability and function of membrane proteins. While experimental approaches to characterize these interactions in a native-like membrane environmen...

Kat Blow has developed CCD2MD, a suite of packages for preparing co-folded outputs for MD simulations.

Created for protein–lipid interactions in integral membrane proteins, it also works for peripheral and lipid-anchored proteins too.

pubs.acs.org/doi/full/10....

github.com/keb721/ccd2md

Bidirectional communication between nucleotide and substrate binding sites in a type IV multidrug ABC transporter Nature Communications - The communication hinge, found in type IV ABC transporters, forms a bidirectional communication between ATP- and substrate-binding sites. This is an unexplored allosteric...

🧪How does an ABC transporter 'know' nucleotides & substrates bound to far apart binding sites? Using 1H15N, 19F NMR, HDX-MS, PET-FCS & activity assays with Neuweiler, Marcoux, Orelle & Jault labs, we found a communication hinge! 😍🥳

@microverse.bsky.social @lifeprofile.bsky.social

rdcu.be/ePm4C

Structural basis of isethionate transport by a TRAP transporter from a sulfate-reducing bacterium Newton-Vesty et al. used a megabody fiducial to determine the cryo-EM structure of an isethionate TRAP transporter, revealing the substrate and Na+-binding sites. Transport is dependent on Na+ and the...

Check out our new paper:
🔗 www.cell.com/structure/fu...

We explored how sulfate-reducing bacteria import isethionate, a sulfur-containing molecule found in the environment and produced by microbes in the human gut. We captured a structure of the IseQM TRAP transporter in a substrate-bound state.

If you’re interested in metals, microbes and maths 🤘 - please check out this PhD on the impact of metals on microbial communities with @thecopperdoctor.bsky.social @blindmath.bsky.social Denis Patterson and myself, you’d be based in Durham & be part of a vibrant interdisciplinary research group

Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.

For more details and to apply please see

www.imperial.ac.uk/jobs/search-...

Biochemical Society's Membrane Protein Conference
7–9 April 2026
Birmingham, UK | Leonardo Royal Hotel

- Abstracts welcome for talks & posters
- Bursaries available!
- Register & submit here:
www.eventsforce.net/biochemsoc/f...

#MembraneProteins #CallForAbstracts @biochemsoc.bsky.social

Check it out! A proper team effort from past and present group members and a great collaboration with @sergemostowylab.bsky.social

Mechanism of phosphoinositide regulation of lysosomal pH via inhibition of CLC-7 https://www.biorxiv.org/content/10.1101/2025.10.01.679551v1

Defined roles for the Staphylococcus aureus POT transporter DtpT in di/tripeptide uptake and glutathione utilisation inside human macrophages Author summary The environments where bacterial pathogens thrive are often rich in proteins and their degradation products, including oligopeptides, which can be taken up by the bacterium and used as ...

Our work deciphering the substrate preferences of a di-/tripeptide transporter from S. aureus is now available as a proof at @plos.org Pathogens! Big thanks to the reviewers and to all the brilliant co-authors, particularly Callum who helped get those last few crucial experiments across the line

A Protein Antibiotic Inhibits the BAM Complex to Kill Without Cell Entry Many antibiotics are ineffective against Gram-negative pathogens such as Pseudomonas aeruginosa because they cannot penetrate the bacterial outer membrane. Here, we show that protein antibiotics calle...

The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.

Small molecule inhibitors of the NorA multidrug efflux pump potentiate antibiotic activity by binding the outward-open conformation Antibiotic resistance is among the greatest threats of the modern era. Multidrug efflux pumps expel antibiotics from bacterial cells and present a particular challenge by conferring resistance to a br...

New preprint from the lab, in collaboration with Ed Tate, Nate Traaseth and many others!

www.biorxiv.org/content/10.1...

We are hiring a Postdoc in membrane protein cryo-EM at the University of Georgia. Looking for candidates with expertise in membrane protein purification and biochemistry. Fully funded for up to 5 years. Join us in Athens, GA. Apply: camilo.perez@uga.edu

This work was driven primarily by a fantastic former MRes student, Lily Jaques, with excellent support from two PhD students Joe Davies and Jack Sheldon-Towler, and some really lovely protein modelling from Vanessa Leone. None of whom are on this platform (I think)!