Spirochrome

Widefield microscope image of HeLa cells stained with SPY488-FastAct

Widefield microscope image of U2OS cells stained with SPY488-FastAct (green), PKmito Orange (white), SPY650-DNA (cyan)

Widefield microscope image of U2OS cells stained with SPY488-Fastact (green) and SPY650-DNA (red)

🟢FastAct™ goes green! 🟢🔬

We launch SPY488-FastAct™ a green channel live cell actin probe

SPY488-FastAct allows imaging F-actin in live and fixed cells

Compatible with SPY555 and SPY650 for easy 3 color imaging.

spirochrome.com/product/spy4...

available at our distributors in a couple of days. 🧪

Widefield microscope image of HeLa cells stained with SPY488-FastAct

Widefield microscope image of U2OS cells stained with SPY488-FastAct (green), PKmito Orange (white), SPY650-DNA (cyan)

Widefield microscope image of U2OS cells stained with SPY488-Fastact (green) and SPY650-DNA (red)

🟢FastAct™ goes green! 🟢🔬

We launch SPY488-FastAct™ a green channel live cell actin probe

SPY488-FastAct allows imaging F-actin in live and fixed cells

Compatible with SPY555 and SPY650 for easy 3 color imaging.

spirochrome.com/product/spy4...

available at our distributors in a couple of days. 🧪

1/New preprint just dropped! 🔥🔥 We investigate how the genome is destroyed in apoptotic cells in a way that prevents DNA fragments from spreading beyond the dying cell 🧬⚰

Done here at @imbavienna.bsky.social & in the super @rcollepardo.bsky.social & Rosen labs:

www.biorxiv.org/content/10.6...

Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday

Easy to use: just dilute the probe in cell culture medium and image 30 min later!

🔗 spirochrome.com/product/spy6...

🙏 Big thanks to @lazaresaladin.bsky.social for the images.
🧪

Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday

Easy to use: just dilute the probe in cell culture medium and image 30 min later!

🔗 spirochrome.com/product/spy6...

🙏 Big thanks to @lazaresaladin.bsky.social for the images.
🧪

We love how amazing our SPY650-DNA probe looks on your microscopy images.
Congratulations to everyone involved!

An amazing preprint by @gerlichlab.bsky.social on the fate of DNA during apoptosis. Definitely to add on your reading list!

Our SPY650-DNA probe was heavily used to visualize DNA throughout the manuscript.

Congratulations to the team!

SPY555-Tubulin and SPY650-FastActX wound up looking good together for 11hrs. Thanks for the helpful info!

Another fiery 💥 probes combination by @j-today.bsky.social, this time with SPY555-Tubulin and SPY650-FastAct_X.

🤩

ICYMI SPY555-FastAct_X and SiR-tubulin in action here! 🤯 16h imaging of neurons 💥
Thank you @j-today.bsky.social for posting this gem!

Amazing movie! We love to see our probes SPY555-FastAct_X and Sir-tubulin perform so well in your hands! 🤩

Thank you for sharing.

Hello epithelial mechanics fans!! I’m Juanma @juanmagararc.bsky.social 👋 I work on cell mechanics (see celldynamicslab.com) and use Flipper-TR FLIM to probe membrane biophysics in cells.

Join me on this tour about Flipper: what it measures, strengths, advice, cool case studies, and cute drawings!

Thank you Kim for your amazing work! We love your designs and working with you on the HAK-actin™ project was fast, efficient and a real pleasure!

📐 Visual communication for @spirochrome.com

Design for the launch of HAK-actin™, a new fluorescent probe for Expansion Microscopy. Project included a clear usage schematic, flyer, and folded leaflet, with a minimalist, efficient style aligned with Spirochrome’s visual identity.

You are correct! mtDNA staining is typically performed with an intercalating DNA dye with nanomolar affinity for dsDNA. SPY-DNA probes, primarily designed for live cell imaging, are DNA groove binders and have a lower affinity to avoid toxicity. Probably not strong enough for robust mtDNA staining.

The images were acquired on a @leicamicrosystems.bsky.social Stellaris system by
@bioarm.bsky.social from @centriolelab.bsky.social.
Huge thank you for trying this out!

The images shows an array of 6 Expansion microscopy images of cell nuclei (three columns and 2 rows). each image was taken with a different SPY-DNA probe to stain the nuclear DNA and is pseudocolored to the fluorescence wavelength of the SPY-DNA probe. Trom top left to bottom right: SPY505-DNA (green); SPY555-DNA (magenta); SPY595-DNA (yellow); SPY620-DNA (orange); SPY650-DNA (red); SPY700-DNA (darker red). a white scale bar of 50 micrometers is given for each image to get an impression of the scale of each nuclei.

Are you staining DNA in your ExM samples?

We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.

You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.

spirochrome.com/products/?_c...

Peroxi_SPY probes were designed for peroxisome live cell imaging. Turns out they work with fixed cells as well!
Peroxi_SPY can thus be combined with immunofluorescence labeling.

A new article provides a detailed protocol: app.jove.com/t/69405/pero...

Products link: spirochrome.com/products/?_c...

Peroxi_SPY probes were designed for peroxisome live cell imaging. Turns out they work with fixed cells as well!
Peroxi_SPY can thus be combined with immunofluorescence labeling.

A new article provides a detailed protocol: app.jove.com/t/69405/pero...

Products link: spirochrome.com/products/?_c...

📄New article!

Fixed cell Peroxisome imaging is now possible with Peroxi_SPY probes
In a new JoVE article Triana Amen, @mschuhmacher.bsky.social and Einat Zalckvar robustly show that Peroxi_SPY stained cells can be fixed and stained by IF!

Detailed protocol in the article link (first reply below⬇️)

A new article by @zhixingchen2.bsky.social shows that the far red mitochondrial probe PKmito Deep Red is a robust mitochondrial membrane potential probe when imaged by FLIM.

www.nature.com/articles/s41...

PKmito Deep Red is exclusively available at Spirochrome:

spirochrome.com/product/pkmi...

New paper from @zhixingchen2.bsky.social's lab!

It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime!

www.nature.com/articles/s41...

The unique mechanosensitive probe Flipper-TR is at the core of an amazing article by @juanmagararc.bsky.social and @rouxlab.bsky.social team of membrane tension experts.

Congratulations!

There is a great 🧵 explaining the key findings how and why cells build membrane tension gradients ⬇️

New paper from the @rouxlab.bsky.social on Nature Communications! We study how membrane tension is spatially organized in cells. Using the mechanosensitive probe Flipper-TR to visualize tension across the plasma membrane of adherent cells and to dissect the conditions needed for a gradient to happen

A new article from Nicolas Plachta's lab with incredible imaging of live human embryos using our probes SPY555-DNA and SPY650-FastAct

www.science.org/doi/full/10....

The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects

The images in the main text are also definitely worth a look!

A new article from Nicolas Plachta's lab with incredible imaging of live human embryos using our probes SPY555-DNA and SPY650-FastAct

www.science.org/doi/full/10....

The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects

Cool, the HAK-actin preprint was showcased by @prelights.bsky.social
A good reason to read it if you haven't yet 😜

Quite a busy day on #Bluesky !
Been a while since we have been #preLight -ed !

Discover HAK-Actin if you haven't already ;)

Thank you @prelights.bsky.social for highlighting HAK-actin!

HAK-actin is a great tool for ExM actin staining.

spirochrome.com/product/hak-...