Nature Protocols

Preparation of targeted lipid nanoparticles for precision nucleic acid delivery Nature Protocols, Published online: 09 March 2026; doi:10.1038/s41596-025-01330-wThis protocol outlines the steps to prepare functionalized lipid nanoparticles covalently conjugated with whole antibodies or antibody fragments for targeted nucleic acid delivery to different tissues or cell types.

New Article! Preparation of targeted lipid nanoparticles for precision nucleic acid delivery

Plasma-coupled electrochemical ammonia synthesis from air and water under ambient conditions Nature Protocols, Published online: 06 March 2026; doi:10.1038/s41596-026-01332-2This Protocol describes an efficient method for producing green ammonia from air and water using a plasma-coupled electrochemical system, offering a sustainable alternative to the Haber–Bosch process and providing valuable guidance for future research.

New Article! Plasma-coupled electrochemical ammonia synthesis from air and water under ambient conditions

A scalable, low-cost, sample hashing workflow for multiomic single-cell analysis using the Seq-Well S3 platform Nature Protocols, Published online: 06 March 2026; doi:10.1038/s41596-025-01308-8This Protocol presents a detailed guide for generating and analyzing single-cell multiomic data from low-input samples with the Seq-Well S3 platform.

New Article! A scalable, low-cost, sample hashing workflow for multiomic single-cell analysis using the Seq-Well S3 platform

The Most Complex Skeletons on Earth? Radiolaria Under SEM YouTube video by Microscopy Specialist

www.youtube.com/watch?v=GhFd...

There is even some methodological information at the end. Just beautiful.

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Our Opening Doors Internship Programme is open for applications! #BePartOfProgress and join one of our teams to gain hands-on experience working on impactful projects and benefit from the expertise of our talented colleagues.
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#Publishing #WorkInPublishing

New Article! A new era for coral functional genomics

New Article! Efficient genome editing using CRISPR–Cas9 in reef-building corals

Oscillatory brain state-dependent stimulation with transcranial magnetic stimulation combined with electroencephalography Nature Protocols, Published online: 27 February 2026; doi:10.1038/s41596-025-01309-7An approach that couples the application of transcranial magnetic stimulation with brain oscillations to improve the effectiveness of long-term plasticity induction.

New Article! Oscillatory brain state-dependent stimulation with transcranial magnetic stimulation combined with electroencephalography

Did you read our paper applying SUM-seq in macrophages, T-cells and iPSCs (www.nature.com/articles/s41...) and think: I want to try this cost-effective single cell RNA & ATAC-seq method myself? 🧬

Find the complete protocol including tips, troubleshooting and timing now in Nature Protocols

Nature News Intern, Springer Nature Opening Doors Programme Nature News Intern, Springer Nature Opening Doors Programme London, UK - Hybrid Working Model Application deadline: 9th March 2026 Are you looking for an internship that provides hands-on experience i...

There is a paid internship for aspiring science journalists at Nature's London office. Up to three days a week working from home. All the details are here (deadline 9 March):

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Voices of experience - Nature Human Behaviour Collective knowledge is a human strength. To benefit from the wisdom of experience, we launch a Series of Comments in a dedicated ‘How to’ style. These ‘How to’ Comments provide brief advice and practical recommendations to researchers across the wide spectrum of disciplines covered by the journal.

A new series of 'How to' Comment is now launched! It provides guidance & practical recommendations for topics of broad interest to our readership. Read our editorial about it:

Single-cell ultra-high-throughput multiplexed chromatin accessibility and gene expression sequencing (SUM-seq) Nature Protocols, Published online: 26 February 2026; doi:10.1038/s41596-025-01310-0A protocol for simultaneous profiling of chromatin accessibility and gene expression in single nuclei that permits sample multiplexing via sample-specific in situ barcoding and high-throughput droplet overloading.

New Article! Single-cell ultra-high-throughput multiplexed chromatin accessibility and gene expression sequencing (SUM-seq)

3D hierarchically aligned nanofiber scaffolds promote cell migration for tissue regeneration Nature Protocols, Published online: 26 February 2026; doi:10.1038/s41596-026-01339-9We present a protocol for the alignment of nanofibers within bioscaffolds to facilitate the recruitment and migration of cells to large wounds, thus improving functional and morphological accuracy in tissue regeneration.

New Article! 3D hierarchically aligned nanofiber scaffolds promote cell migration for tissue regeneration

Volumetric DNA microscopy for mapping spatial transcriptomes in three dimensions Nature Protocols, Published online: 24 February 2026; doi:10.1038/s41596-025-01329-3This protocol for encoding spatial information via DNA sequences enables template-free reconstruction of three-dimensional gene expression patterns in intact specimens, thereby overcoming the limitations of two-dimensional spatial imaging.

New Article! Volumetric DNA microscopy for mapping spatial transcriptomes in three dimensions

Aqueous sonochemical synthesis of covalent organic frameworks Nature Protocols, Published online: 23 February 2026; doi:10.1038/s41596-025-01323-9Covalent organic frameworks (COFs) could be used for diverse applications. The dominant solvothermal method for COF synthesis requires harsh conditions for long periods of time. This Protocol describes an aqueous sonochemical approach for imine-based COFs.

New Article! Aqueous sonochemical synthesis of covalent organic frameworks

High-throughput reaction screening using acoustic ejection mass spectrometry Nature Protocols, Published online: 23 February 2026; doi:10.1038/s41596-025-01320-yA bottleneck in high-throughput chemical synthesis is analyzing reaction products. This protocol describes a direct mass spectrometry approach where the loss of a characteristic neutral fragment from a starting material defines product analysis.

New Article! High-throughput reaction screening using acoustic ejection mass spectrometry

Harnessing intermediate-scale bioreactors for next-generation macrophage production and application Nature Protocols, Published online: 18 February 2026; doi:10.1038/s41596-025-01313-xThis Protocol Extension details the standardized production of iPS cell-derived macrophages in an intermediate-scale benchtop bioreactor, providing a valuable tool for academic labs focused on human immune cells.

New Article! Harnessing intermediate-scale bioreactors for next-generation macrophage production and application

Super happy to share our protocol for ENGRAM is out in Nature Protocols! This was fun to write with @chenomics.bsky.social @troymcdiarmid.bsky.social @jshendure.bsky.social. In it we describe how to record CRE identity, activity, and order in mammalian cells.

New paper just out, based on new ISO method for animal-free food protein digestibility/DIAAS method. Lead-authors R. Portman and L. Egger, great long-term effort. I am so happy to be part this process #infogest
@vistamilk.bsky.social #giantleaps @csic.es @researchireland.ie @teagasc.bsky.social

Check out our new protocol paper on molecular recording of transcriptional events in genomic DNA!

Super fun pulling this together with @jennynathans.bsky.social @chenomics.bsky.social and @jshendure.bsky.social

Preparing and characterizing environmentally aged microplastics Nature Protocols, Published online: 17 February 2026; doi:10.1038/s41596-025-01319-5Microplastics (MPs) are persistent and widely distributed pollutants. Environmental factors cause physical and chemical aging that change their properties. This protocol is a systematic workflow for controlled MP aging and its characterization.

New Article! Preparing and characterizing environmentally aged microplastics

Drosophila melanogaster as a model host for studying Pseudomonas aeruginosa feeding infection Nature Protocols, Published online: 16 February 2026; doi:10.1038/s41596-025-01311-zThis Protocol Extension describes the infection of adult Drosophila with Pseudomonas aeruginosa by feeding, as opposed to a previous protocol that used needle-pricking or injector-pumping to impose a wound or a direct systemic infection, respectively.

New Article! Drosophila melanogaster as a model host for studying Pseudomonas aeruginosa feeding infection

High-throughput identification of endogenous biomolecular condensates and phase-separating proteins Nature Protocols, Published online: 12 February 2026; doi:10.1038/s41596-025-01327-5This protocol identifies endogenous biomolecular condensates by combining sucrose density gradient centrifugation with quantitative mass spectrometry, enabling proteome-wide, stimulus-responsive profiling of phase-separating proteins.

New Article! High-throughput identification of endogenous biomolecular condensates and phase-separating proteins

New Article! Multichannel genomic recording of biological information with ENGRAM

Simplified ischemia-free liver transplantation with continuous normothermic machine perfusion Nature Protocols, Published online: 06 February 2026; doi:10.1038/s41596-025-01321-xThis Protocol describes a simplified ischemia-free liver transplant procedure, where the portal vein is directly cannulated into the donor portal vein, eliminating the need for an interposition vein.

New Article! Simplified ischemia-free liver transplantation with continuous normothermic machine perfusion

New Article! Designing and testing CRISPRi-based synthetic gene circuits in plants

Conjugation of hydrophobic drugs to motile pRNA 4WJ nanoparticles for spontaneous tumor targeting and undetectable toxicity Nature Protocols, Published online: 03 February 2026; doi:10.1038/s41596-025-01306-wThis protocol describes the synthesis, purification and assembly of stable branched 4-way junction (4WJ) RNA nanoparticles conjugated with multiple hydrophobic chemotherapeutic drug molecules to target specific tumors with minimal side effects.

New Article! Conjugation of hydrophobic drugs to motile pRNA 4WJ nanoparticles for spontaneous tumor targeting and undetectable toxicity

Secreted luciferases as a minimally invasive 3R-compliant tool for accurate monitoring of tumor burden Nature Protocols, Published online: 03 February 2026; doi:10.1038/s41596-025-01315-9This protocol details in vitro and in vivo approaches for labeling tumor cells with secreted luciferases to enable precise, longitudinal monitoring of tumor growth in mice, through the analysis of small blood samples.

New Article! Secreted luciferases as a minimally invasive 3R-compliant tool for accurate monitoring of tumor burden

INFOGEST Quant: standardized in vitro determination of digestibility and DIAAS of dietary proteins based on the INFOGEST static digestion model Nature Protocols, Published online: 03 February 2026; doi:10.1038/s41596-025-01307-9This Protocol Extension is an adapted version of the authors’ previous INFOGEST static digestion model (INFOGEST 2.0), with an added workflow for the quantification of total protein digestibility, individual amino acid digestibility and digestible indispensable amino acid score.

New Article! INFOGEST Quant: standardized in vitro determination of digestibility and DIAAS of dietary proteins based on the INFOGEST static digestion model

Magnetoencephalography in human neuroscience research: planning, piloting, implementation and quality assurance Nature Protocols, Published online: 02 February 2026; doi:10.1038/s41596-025-01295-wA protocol for the planning and implementation of magnetoencephalography experiments across nonclinical and clinical settings, including the methodological considerations required for designing multidisciplinary, population-based studies.

New Article! Magnetoencephalography in human neuroscience research: planning, piloting, implementation and quality assurance